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. 1990 Jun;28(6):1214–1218. doi: 10.1128/jcm.28.6.1214-1218.1990

Use of a novel phosphatase test for simplified identification of species of the tribe Proteeae.

R Pompei 1, G Cornaglia 1, A Ingianni 1, G Satta 1
PMCID: PMC267907  PMID: 2166077

Abstract

Phosphatase activity of 334 isolates of the tribe Proteeae carefully identified to species level has been evaluated by using both a number of conventional tests and three versions of the novel methyl green-phenolphthalein (MGP) method (G. Satta. R. Pompei, G. Grazi, and G. Cornaglia, J. Clin. Microbiol. 26:2637-2641, 1988). We found that the different species of Proteeae show different and easily distinguishable. behaviors by the MGP method, while all of them behave in a uniform way in the conventional tests. On studying the mechanism underlying these different behaviors, we found that the phosphatase activity of Morganella morganii and Providencia stuartii is higher than that of all other members of the family Enterobacteriaceae. Furthermore, we found that all P. stuartii strains display the property (shared by virtually none of the other species of Enterobacteriaceae) of excreting significant amounts of phosphatase. This extracellular activity is reliably detected by the MGP method but not by conventional tests. Finally, by exploiting the peculiar phosphatase activity of this tribe, as revealed by the MGP method, we devised a simple scheme for routine identification of the species of Proteeae and evaluated its reliability by comparing it with three commonly used commercial kits. The new scheme proved much simpler, but also more reliable, since identifications obtained by this method were in almost complete accord (99%) with those of the reference identification schemes, while in the commercial systems examined the percentage of errors ranged from 13 to 14 in the identification of Providencia species.

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Selected References

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  1. BAIRD-PARKER A. C. A classification of micrococci and staphylococci based on physiological and biochemical tests. J Gen Microbiol. 1963 Mar;30:409–427. doi: 10.1099/00221287-30-3-409. [DOI] [PubMed] [Google Scholar]
  2. BARBER M., KUPER S. W. A. Identification of Staphylococcus pyogenes by the phosphatase reaction. J Pathol Bacteriol. 1951 Jan;63(1):65–68. doi: 10.1002/path.1700630108. [DOI] [PubMed] [Google Scholar]
  3. Bobey D. G., Ederer G. M. Rapid detection of yeast enzymes by using 4-methylumbelliferyl substrates. J Clin Microbiol. 1981 Feb;13(2):393–394. doi: 10.1128/jcm.13.2.393-394.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cornaglia G., Dainelli B., Berlutti F., Thaller M. C. Commercial identification systems often fail to identify Providencia stuartii. J Clin Microbiol. 1988 Feb;26(2):323–327. doi: 10.1128/jcm.26.2.323-327.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cornaglia G., Pompei R., Dainelli B., Satta G. In vitro activity of ciprofloxacin against aerobic bacteria isolated in a southern European hospital. Antimicrob Agents Chemother. 1987 Oct;31(10):1651–1655. doi: 10.1128/aac.31.10.1651. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Facklam R. R. Physiological differentiation of viridans streptococci. J Clin Microbiol. 1977 Feb;5(2):184–201. doi: 10.1128/jcm.5.2.184-201.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Farmer J. J., 3rd, Davis B. R., Hickman-Brenner F. W., McWhorter A., Huntley-Carter G. P., Asbury M. A., Riddle C., Wathen-Grady H. G., Elias C., Fanning G. R. Biochemical identification of new species and biogroups of Enterobacteriaceae isolated from clinical specimens. J Clin Microbiol. 1985 Jan;21(1):46–76. doi: 10.1128/jcm.21.1.46-76.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hawkey P. M., Pedler S. J., Turner A. Comparative in vitro activity of semisynthetic penicillins against Proteeae. Antimicrob Agents Chemother. 1983 Apr;23(4):619–621. doi: 10.1128/aac.23.4.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hawkey P. M. Providencia stuartii: a review of a multiply antibiotic-resistant bacterium. J Antimicrob Chemother. 1984 Mar;13(3):209–226. doi: 10.1093/jac/13.3.209. [DOI] [PubMed] [Google Scholar]
  10. Holt R. The classification of staphylococci from colonized ventriculo-atrial shunts. J Clin Pathol. 1969 Jul;22(4):475–482. doi: 10.1136/jcp.22.4.475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. IVANOVICS G., FOLDES J. Problems concerning the phylogenesis of Bacillus anthracis. Acta Microbiol Acad Sci Hung. 1958;5(1):89–109. [PubMed] [Google Scholar]
  12. Kilian M. A taxonomic study of the genus Haemophilus, with the proposal of a new species. J Gen Microbiol. 1976 Mar;93(1):9–62. doi: 10.1099/00221287-93-1-9. [DOI] [PubMed] [Google Scholar]
  13. Penner J. L., Preston M. A., Hennessy J. N., Barton L. J., Goodbody M. M. Species differences in susceptibilities of Proteeae spp. to six cephalosporins and three aminoglycosides. Antimicrob Agents Chemother. 1982 Aug;22(2):218–221. doi: 10.1128/aac.22.2.218. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Porschen R. K., Spaulding E. H. Phosphatase activity of anaerobic organisms. Appl Microbiol. 1974 Apr;27(4):744–747. doi: 10.1128/am.27.4.744-747.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Satta G., Grazi G., Varaldo P. E., Fontana R. Detection of bacterial phosphatase activity by means of an original and simple test. J Clin Pathol. 1979 Apr;32(4):391–395. doi: 10.1136/jcp.32.4.391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Satta G., Pompei R., Grazi G., Cornaglia G. Phosphatase activity is a constant feature of all isolates of all major species of the family Enterobacteriaceae. J Clin Microbiol. 1988 Dec;26(12):2637–2641. doi: 10.1128/jcm.26.12.2637-2641.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Satta G., Pompei R., Ingianni A. The selective staining mechanism of phosphatase producing colonies in the diphosphatephenolphthalein-methyl green method for the detection of bacterial phosphatase activity. Microbiologica. 1984 Apr;7(2):159–170. [PubMed] [Google Scholar]
  18. Taylor R. K., Miller V. L., Furlong D. B., Mekalanos J. J. Use of phoA gene fusions to identify a pilus colonization factor coordinately regulated with cholera toxin. Proc Natl Acad Sci U S A. 1987 May;84(9):2833–2837. doi: 10.1073/pnas.84.9.2833. [DOI] [PMC free article] [PubMed] [Google Scholar]

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