Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1990 Jun;28(6):1244–1248. doi: 10.1128/jcm.28.6.1244-1248.1990

Antigenic characterization of small, round-structured viruses by immune electron microscopy.

S Okada 1, S Sekine 1, T Ando 1, Y Hayashi 1, M Murao 1, K Yabuuchi 1, T Miki 1, M Ohashi 1
PMCID: PMC267912  PMID: 2380353

Abstract

Small, round-structured viruses (SRSVs) detected from nonbacterial gastroenteritis outbreaks in Tokyo and Saitama Prefecture, Japan, during the period from 1977 to 1988 were tentatively classified into nine antigenic patterns from SRSV-1 (S-1) to SRSV-9 (S-9) by cross-immune electron microscopy (IEM). S-1 and S-2 appeared pattern specific, while S-3 to S-9, distinguishable from each other in their reactivity, appeared somewhat antigenically related. Their antigenic relatedness to the Norwal, Hawaii, and Otofuke agents was also examined by IEM by using antisera to these agents. S-3 appeared most closely related to the Norwalk agent. S-4 and S-5 were related to the Norwalk agent and, presumably, were distantly related to the Hawaii and Otofuke agents. S-6 and S-7 were related to the Hawaii and Otofuke agents. S-8 and S-9 were related to the Otofuke agent and, presumably, were distantly related to the Hawaii agent. The prevalence of each antigenic pattern in 38 outbreaks was examined: S-8 was implicated in 24% of the outbreaks S-5 in 16%, S-4 in 13%, S-9 in 13%, S-6 in 11%, and others in 5%.

Full text

PDF
1244

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Appleton H. Small round viruses: classification and role in food-borne infections. Ciba Found Symp. 1987;128:108–125. doi: 10.1002/9780470513460.ch7. [DOI] [PubMed] [Google Scholar]
  2. Caul E. O., Appleton H. The electron microscopical and physical characteristics of small round human fecal viruses: an interim scheme for classification. J Med Virol. 1982;9(4):257–265. doi: 10.1002/jmv.1890090403. [DOI] [PubMed] [Google Scholar]
  3. Christensen M. L. Human viral gastroenteritis. Clin Microbiol Rev. 1989 Jan;2(1):51–89. doi: 10.1128/cmr.2.1.51. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cubitt W. D., Blacklow N. R., Herrmann J. E., Nowak N. A., Nakata S., Chiba S. Antigenic relationships between human caliciviruses and Norwalk virus. J Infect Dis. 1987 Nov;156(5):806–814. doi: 10.1093/infdis/156.5.806. [DOI] [PubMed] [Google Scholar]
  5. Dolin R., Reichman R. C., Roessner K. D., Tralka T. S., Schooley R. T., Gary W., Morens D. Detection by immune electron microscopy of the Snow Mountain agent of acute viral gastroenteritis. J Infect Dis. 1982 Aug;146(2):184–189. doi: 10.1093/infdis/146.2.184. [DOI] [PubMed] [Google Scholar]
  6. Dolin R., Roessner K. D., Treanor J. J., Reichman R. C., Phillips M., Madore H. P. Radioimmunoassay for detection of the Snow Mountain Agent of viral gastroenteritis. J Med Virol. 1986 May;19(1):11–18. doi: 10.1002/jmv.1890190103. [DOI] [PubMed] [Google Scholar]
  7. Greenberg H. B., Wyatt R. G., Valdesuso J., Kalica A. R., London W. T., Chanock R. M., Kapikian A. Z. Solid-phase microtiter radioimmunoassay for detection of the Norwalk strain of acute nonbacterial, epidemic gastroenteritis virus and its antibodies. J Med Virol. 1978;2(2):97–108. doi: 10.1002/jmv.1890020204. [DOI] [PubMed] [Google Scholar]
  8. Grohmann G. S., Greenberg H. B., Welch B. M., Murphy A. M. Oyster-associated gastroenteritis in Australia: the detection of Norwalk virus and its antibody by immune electron microscopy and radioimmunoassay. J Med Virol. 1980;6(1):11–19. doi: 10.1002/jmv.1890060103. [DOI] [PubMed] [Google Scholar]
  9. Hayashi Y., Ando T., Utagawa E., Sekine S., Okada S., Yabuuchi K., Miki T., Ohashi M. Western blot (immunoblot) assay of small, round-structured virus associated with an acute gastroenteritis outbreak in Tokyo. J Clin Microbiol. 1989 Aug;27(8):1728–1733. doi: 10.1128/jcm.27.8.1728-1733.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kapikian A. Z., Wyatt R. G., Dolin R., Thornhill T. S., Kalica A. R., Chanock R. M. Visualization by immune electron microscopy of a 27-nm particle associated with acute infectious nonbacterial gastroenteritis. J Virol. 1972 Nov;10(5):1075–1081. doi: 10.1128/jvi.10.5.1075-1081.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kaplan J. E., Gary G. W., Baron R. C., Singh N., Schonberger L. B., Feldman R., Greenberg H. B. Epidemiology of Norwalk gastroenteritis and the role of Norwalk virus in outbreaks of acute nonbacterial gastroenteritis. Ann Intern Med. 1982 Jun;96(6 Pt 1):756–761. doi: 10.7326/0003-4819-96-6-756. [DOI] [PubMed] [Google Scholar]
  12. Lewis D. C., Lightfoot N. F., Pether J. V. Solid-phase immune electron microscopy with human immunoglobulin M for serotyping of Norwalk-like viruses. J Clin Microbiol. 1988 May;26(5):938–942. doi: 10.1128/jcm.26.5.938-942.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Murphy A. M., Grohmann G. S., Christopher P. J., Lopez W. A., Davey G. R., Millsom R. H. An Australia-wide outbreak of gastroenteritis from oysters caused by Norwalk virus. Med J Aust. 1979 Oct 6;2(7):329–333. doi: 10.5694/j.1326-5377.1979.tb104133.x. [DOI] [PubMed] [Google Scholar]
  14. Pether J. V., Caul E. O. An outbreak of food-borne gastroenteritis in two hospitals associated with a Norwalk-like virus. J Hyg (Lond) 1983 Oct;91(2):343–350. doi: 10.1017/s0022172400060368. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sekine S., Okada S., Hayashi Y., Ando T., Terayama T., Yabuuchi K., Miki T., Ohashi M. Prevalence of small round structured virus infections in acute gastroenteritis outbreaks in Tokyo. Microbiol Immunol. 1989;33(3):207–217. doi: 10.1111/j.1348-0421.1989.tb01514.x. [DOI] [PubMed] [Google Scholar]
  16. Taniguchi K., Urasawa S., Urasawa T. Further studies of 35--40 nm virus-like particles associated with outbreaks of acute gastroenteritis. J Med Microbiol. 1981 Feb;14(1):107–118. doi: 10.1099/00222615-14-1-107. [DOI] [PubMed] [Google Scholar]
  17. Taniguchi K., Urasawa S., Urasawa T. Virus-like particle, 35 to 40 nm, associated with an institutional outbreak of acute gastroenteritis in adults. J Clin Microbiol. 1979 Nov;10(5):730–736. doi: 10.1128/jcm.10.5.730-736.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Thornhill T. S., Wyatt R. G., Kalica A. R., Dolin R., Chanock R. M., Kapikian A. Z. Detection by immune electron microscopy of 26- to 27-nm viruslike particles associated with two family outbreaks of gastroenteritis. J Infect Dis. 1977 Jan;135(1):20–27. doi: 10.1093/infdis/135.1.20. [DOI] [PubMed] [Google Scholar]
  19. Wyatt R. G., Dolin R., Blacklow N. R., DuPont H. L., Buscho R. F., Thornhill T. S., Kapikian A. Z., Chanock R. M. Comparison of three agents of acute infectious nonbacterial gastroenteritis by cross-challenge in volunteers. J Infect Dis. 1974 Jun;129(6):709–714. doi: 10.1093/infdis/129.6.709. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES