Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1990 Jun;28(6):1261–1264. doi: 10.1128/jcm.28.6.1261-1264.1990

Serological response to Chlamydia pneumoniae infection.

L A Campbell 1, C C Kuo 1, S P Wang 1, J T Grayston 1
PMCID: PMC267915  PMID: 2380354

Abstract

The human serological response was analyzed by using sera from patients who were serologically positive but isolation negative for Chlamydia pneumoniae and from patients with proven C. pneumoniae infection based on serology and isolation. To assess whether seroreactivity to C. pneumoniae proteins had potential diagnostic value, the cross-reactivities of these sera to other Chlamydia species and of sera from patients infected with C. trachomatis and C. psittaci to C. pneumoniae proteins were determined. In all serum samples from patients with proven C. pneumoniae infections, reactivities were seen with 98-, 68-, 60-, 39.5-, and 30-kilodalton proteins. Similar patterns were seen in sera from patients who were serologically positive and isolation negative. The onset of seropositivity for C. pneumoniae was accompanied by reactivities against presumably shared chlamydial antigens and a C. pneumoniae-specific 98-kilodalton protein.

Full text

PDF
1261

Images in this article

1263Image
on p.1263

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baehr W., Zhang Y. X., Joseph T., Su H., Nano F. E., Everett K. D., Caldwell H. D. Mapping antigenic domains expressed by Chlamydia trachomatis major outer membrane protein genes. Proc Natl Acad Sci U S A. 1988 Jun;85(11):4000–4004. doi: 10.1073/pnas.85.11.4000. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brunham R. C., Peeling R., Maclean I., McDowell J., Persson K., Osser S. Postabortal Chlamydia trachomatis salpingitis: correlating risk with antigen-specific serological responses and with neutralization. J Infect Dis. 1987 Apr;155(4):749–755. doi: 10.1093/infdis/155.4.749. [DOI] [PubMed] [Google Scholar]
  3. Campbell L. A., Kuo C. C., Grayston J. T. Structural and antigenic analysis of Chlamydia pneumoniae. Infect Immun. 1990 Jan;58(1):93–97. doi: 10.1128/iai.58.1.93-97.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Campbell L. A., Kuo C. C., Thissen R. W., Grayston J. T. Isolation of a gene encoding a Chlamydia sp. strain TWAR protein that is recognized during infection of humans. Infect Immun. 1989 Jan;57(1):71–75. doi: 10.1128/iai.57.1.71-75.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Clarke I. N., Ward M. E., Lambden P. R. Molecular cloning and sequence analysis of a developmentally regulated cysteine-rich outer membrane protein from Chlamydia trachomatis. Gene. 1988 Nov 30;71(2):307–314. doi: 10.1016/0378-1119(88)90047-9. [DOI] [PubMed] [Google Scholar]
  6. Conlan J. W., Clarke I. N., Ward M. E. Epitope mapping with solid-phase peptides: identification of type-, subspecies-, species- and genus-reactive antibody binding domains on the major outer membrane protein of Chlamydia trachomatis. Mol Microbiol. 1988 Sep;2(5):673–679. doi: 10.1111/j.1365-2958.1988.tb00076.x. [DOI] [PubMed] [Google Scholar]
  7. GORDON F. B., QUAN A. L. OCCURENCE OF GLYCOGEN IN INCLUSIONS OF THE PSITTACOSIS-LYMPHOGRANULOMA VENEREUM-TRACHOMA AGENTS. J Infect Dis. 1965 Apr;115:186–196. doi: 10.1093/infdis/115.2.186. [DOI] [PubMed] [Google Scholar]
  8. Grayston J. T., Kuo C. C., Wang S. P., Altman J. A new Chlamydia psittaci strain, TWAR, isolated in acute respiratory tract infections. N Engl J Med. 1986 Jul 17;315(3):161–168. doi: 10.1056/NEJM198607173150305. [DOI] [PubMed] [Google Scholar]
  9. Grayston J. T., Mordhorst C., Bruu A. L., Vene S., Wang S. P. Countrywide epidemics of Chlamydia pneumoniae, strain TWAR, in Scandinavia, 1981-1983. J Infect Dis. 1989 Jun;159(6):1111–1114. doi: 10.1093/infdis/159.6.1111. [DOI] [PubMed] [Google Scholar]
  10. Grayston J. T., Wang S. P., Kuo C. C., Campbell L. A. Current knowledge on Chlamydia pneumoniae, strain TWAR, an important cause of pneumonia and other acute respiratory diseases. Eur J Clin Microbiol Infect Dis. 1989 Mar;8(3):191–202. doi: 10.1007/BF01965260. [DOI] [PubMed] [Google Scholar]
  11. Howard L., Orenstein N. S., King N. W. Purification on renografin density gradients of Chlamydia trachomatis grown in the yolk sac of eggs. Appl Microbiol. 1974 Jan;27(1):102–106. doi: 10.1128/am.27.1.102-106.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kleemola M., Saikku P., Visakorpi R., Wang S. P., Grayston J. T. Epidemics of pneumonia caused by TWAR, a new Chlamydia organism, in military trainees in Finland. J Infect Dis. 1988 Feb;157(2):230–236. doi: 10.1093/infdis/157.2.230. [DOI] [PubMed] [Google Scholar]
  13. Kuo C. C., Chen H. H., Wang S. P., Grayston J. T. Identification of a new group of Chlamydia psittaci strains called TWAR. J Clin Microbiol. 1986 Dec;24(6):1034–1037. doi: 10.1128/jcm.24.6.1034-1037.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  15. Ladany S., Black C. M., Farshy C. E., Ossewaarde J. M., Barnes R. C. Enzyme immunoassay to determine exposure to Chlamydia pneumoniae (strain TWAR). J Clin Microbiol. 1989 Dec;27(12):2778–2783. doi: 10.1128/jcm.27.12.2778-2783.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  17. Maclean I. W., Peeling R. W., Brunham R. C. Characterization of Chlamydia trachomatis antigens with monoclonal and polyclonal antibodies. Can J Microbiol. 1988 Feb;34(2):141–147. doi: 10.1139/m88-028. [DOI] [PubMed] [Google Scholar]
  18. Newhall W. J., Batteiger B., Jones R. B. Analysis of the human serological response to proteins of Chlamydia trachomatis. Infect Immun. 1982 Dec;38(3):1181–1189. doi: 10.1128/iai.38.3.1181-1189.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Saikku P., Wang S. P., Kleemola M., Brander E., Rusanen E., Grayston J. T. An epidemic of mild pneumonia due to an unusual strain of Chlamydia psittaci. J Infect Dis. 1985 May;151(5):832–839. doi: 10.1093/infdis/151.5.832. [DOI] [PubMed] [Google Scholar]
  20. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wenman W. M., Lovett M. A. Expression in E. coli of Chlamydia trachomatis antigen recognized during human infection. Nature. 1982 Mar 4;296(5852):68–70. doi: 10.1038/296068a0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES