Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1990 Jun;28(6):1265–1270. doi: 10.1128/jcm.28.6.1265-1270.1990

Restriction endonuclease analysis of Eikenella corrodens.

C K Chen 1, G J Sunday 1, J J Zambon 1, M E Wilson 1
PMCID: PMC267916  PMID: 2166079

Abstract

Eikenella corrodens is a gram-negative facultative bacillus commonly found in the oral cavity. Although the role of E. corrodens in periodonititis is not clear, its isolation from extraoral infections attests to its pathogenic potential. Previous studies suggested that this species is phenotypically diverse. In the present study, we used restriction endonuclease analysis (REA) to assess the genetic diversity of this species and to explore the applicability of REA in studying the transmission of E. corrodens. Two groups of E. corrodens isolates were used in this study. Group 1 included 47 epidemiologically independent isolates recovered from dental plaques in periodontally healthy subjects and periodontitis patients and from extraoral infections in different geographic areas. Group 2 E. corrodens included 40 isolates recovered from two periodontitis patients and two periodontally healthy subjects. The results indicated that E. corrodens is genetically heterogeneous, as determined by REA. The majority of the group 1 E. corrodens isolates exhibited strain-specific restriction patterns. Forty restriction patterns were distinguishable among the 47 isolates. Analyses of group 2 isolates revealed that three of four subjects harbored more than one clonal type of E. corrodens. In one instance, a periodontitis patient was found to be colonized by six different clones. Furthermore, two different clonal types of E. corrodens were recovered from a single periodontal pocket in this patient. The results indicated that REA may be a useful tool in the epidemiologic investigation of E. corrodens infections.

Full text

PDF
1265

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradbury W. C., Pearson A. D., Marko M. A., Congi R. V., Penner J. L. Investigation of a Campylobacter jejuni outbreak by serotyping and chromosomal restriction endonuclease analysis. J Clin Microbiol. 1984 Mar;19(3):342–346. doi: 10.1128/jcm.19.3.342-346.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brooks G. F., O'Donoghue J. M., Rissing J. P., Soapes K., Smith J. W. Eikenella corrodens, a recently recognized pathogen: infections in medical-surgical patients and in association with methylphenidate abuse. Medicine (Baltimore) 1974 Sep;53(5):325–342. [PubMed] [Google Scholar]
  3. Chen C. K., Dunford R. G., Reynolds H. S., Zambon J. J. Eikenella corrodens in the human oral cavity. J Periodontol. 1989 Nov;60(11):611–616. doi: 10.1902/jop.1989.60.11.611. [DOI] [PubMed] [Google Scholar]
  4. Chen C., Wilson M. E. Variable sensitivity of oral isolates of Eikenella corrodens to serum bactericidal activity: role of antibody. Adv Dent Res. 1988 Nov;2(2):346–353. doi: 10.1177/08959374880020022501. [DOI] [PubMed] [Google Scholar]
  5. Coykendall A. L., Kaczmarek F. S. DNA homologies among Eikenella corrodens strains. J Periodontal Res. 1980 Nov;15(6):615–620. doi: 10.1111/j.1600-0765.1980.tb00320.x. [DOI] [PubMed] [Google Scholar]
  6. Drouet E., De Montclos H., Boude M., Denoyel G. A. Eikenella corrodens and intrauterine contraceptive device. Lancet. 1987 Nov 7;2(8567):1089–1089. doi: 10.1016/s0140-6736(87)91517-0. [DOI] [PubMed] [Google Scholar]
  7. Finlay B. B., Falkow S. Common themes in microbial pathogenicity. Microbiol Rev. 1989 Jun;53(2):210–230. doi: 10.1128/mr.53.2.210-230.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Goldstein E. J., Tarenzi L. A., Agyare E. O., Berger J. R. Prevalence of Eikenella corrodens in dental plaque. J Clin Microbiol. 1983 Apr;17(4):636–639. doi: 10.1128/jcm.17.4.636-639.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Göthe E., Mutters R., Mannheim W. Deoxyribonucleic acid relatedness and phenotypic variation among human isolates of Eikenella corrodens. Zentralbl Bakteriol. 1989 May;271(1):61–69. doi: 10.1016/s0934-8840(89)80054-4. [DOI] [PubMed] [Google Scholar]
  10. Hartl D. L., Dykhuizen D. E. The population genetics of Escherichia coli. Annu Rev Genet. 1984;18:31–68. doi: 10.1146/annurev.ge.18.120184.000335. [DOI] [PubMed] [Google Scholar]
  11. Kulkarni G. V., Chan K. H., Sandham H. J. An investigation into the use of restriction endonuclease analysis for the study of transmission of mutans streptococci. J Dent Res. 1989 Jul;68(7):1155–1161. doi: 10.1177/00220345890680070401. [DOI] [PubMed] [Google Scholar]
  12. Mandell R. L., Ebersole J. L., Socransky S. S. Clinical immunologic and microbiologic features of active disease sites in juvenile periodontitis. J Clin Periodontol. 1987 Oct;14(9):534–540. doi: 10.1111/j.1600-051x.1987.tb00996.x. [DOI] [PubMed] [Google Scholar]
  13. Moore W. E., Holdeman L. V., Cato E. P., Smibert R. M., Burmeister J. A., Palcanis K. G., Ranney R. R. Comparative bacteriology of juvenile periodontitis. Infect Immun. 1985 May;48(2):507–519. doi: 10.1128/iai.48.2.507-519.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Murphy T. F., Dudas K. C., Mylotte J. M., Apicella M. A. A subtyping system for nontypable Haemophilus influenzae based on outer-membrane proteins. J Infect Dis. 1983 May;147(5):838–846. doi: 10.1093/infdis/147.5.838. [DOI] [PubMed] [Google Scholar]
  15. Orskov F., Orskov I. From the national institutes of health. Summary of a workshop on the clone concept in the epidemiology, taxonomy, and evolution of the enterobacteriaceae and other bacteria. J Infect Dis. 1983 Aug;148(2):346–357. doi: 10.1093/infdis/148.2.346. [DOI] [PubMed] [Google Scholar]
  16. Perez Trallero E., Garcia Arenzana J. M., Cilla Eguiluz G., de Toro Rios P. Extraoral origin of Eikenella corrodens infection. Lancet. 1988 Feb 6;1(8580):298–299. doi: 10.1016/s0140-6736(88)90378-9. [DOI] [PubMed] [Google Scholar]
  17. Savitt E. D., Socransky S. S. Distribution of certain subgingival microbial species in selected periodontal conditions. J Periodontal Res. 1984 Mar;19(2):111–123. doi: 10.1111/j.1600-0765.1984.tb00800.x. [DOI] [PubMed] [Google Scholar]
  18. Selander R. K., Caugant D. A., Ochman H., Musser J. M., Gilmour M. N., Whittam T. S. Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics. Appl Environ Microbiol. 1986 May;51(5):873–884. doi: 10.1128/aem.51.5.873-884.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stoloff A. L., Gillies M. L. Infections with Eikenella corrodens in a general hospital: a report of 33 cases. Rev Infect Dis. 1986 Jan-Feb;8(1):50–53. doi: 10.1093/clinids/8.1.50. [DOI] [PubMed] [Google Scholar]
  20. Suwanagool S., Rothkopf M. M., Smith S. M., LeBlanc D., Eng R. Pathogenicity of Eikenella corrodens in humans. Arch Intern Med. 1983 Dec;143(12):2265–2268. [PubMed] [Google Scholar]
  21. Tanner A. C., Haffer C., Bratthall G. T., Visconti R. A., Socransky S. S. A study of the bacteria associated with advancing periodontitis in man. J Clin Periodontol. 1979 Oct;6(5):278–307. doi: 10.1111/j.1600-051x.1979.tb01931.x. [DOI] [PubMed] [Google Scholar]
  22. Zambon J. J., Sunday G. J., Smutko J. S. Molecular genetic analysis of Actinobacillus actinomycetemcomitans epidemiology. J Periodontol. 1990 Feb;61(2):75–80. doi: 10.1902/jop.1990.61.2.75. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES