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. 1990 Jun;28(6):1288–1291. doi: 10.1128/jcm.28.6.1288-1291.1990

Comparison of improved BACTEC and Lowenstein-Jensen media for culture of mycobacteria from clinical specimens.

P Anargyros 1, D S Astill 1, I S Lim 1
PMCID: PMC267921  PMID: 2116446

Abstract

A 4-month trial involving 2,563 routine clinical specimens was conducted to compare the improved BACTEC TB system (12B medium) with the conventional Lowenstein-Jensen (LJ) media for the isolation, identification, and susceptibility testing of mycobacteria. One hundred sixty-two mycobacterial isolates were recovered, 147 (91%) with BACTEC and 118 (73%) with LJ media. Of these, 62 were Mycobacterium tuberculosis complex strains, 59 (95%) of which were isolated with BACTEC and 54 (87%) of which were isolated with LJ media. Of the remaining 100 isolates, which were mycobacteria other than tuberculosis (MOTT), BACTEC and LJ media detected 88 and 64%, respectively. The contamination rate was significantly higher in BACTEC (5%) than in LJ media (3.3%). The mean isolation time for M. tuberculosis complex with BACTEC was 15.5 days, compared with 25.6 days with LJ. For MOTT, the mean isolation times were 5.8 and 21.4 days, respectively. Identification of 32 M. tuberculosis complex isolates and 38 isolates of MOTT by the BACTEC NAP (p-nitro-alpha-acetylamino-beta-hydroxypropiophenone) inhibition test gave 100% agreement with conventional biochemical identifications. The results of susceptibility testing of 18 M. tuberculosis complex isolates with BACTEC agreed completely with those obtained by the resistance ratio method.

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Selected References

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  1. CANETTI G., FROMAN S., GROSSET J., HAUDUROY P., LANGEROVA M., MAHLER H. T., MEISSNER G., MITCHISON D. A., SULA L. MYCOBACTERIA: LABORATORY METHODS FOR TESTING DRUG SENSITIVITY AND RESISTANCE. Bull World Health Organ. 1963;29:565–578. [PMC free article] [PubMed] [Google Scholar]
  2. Kirihara J. M., Hillier S. L., Coyle M. B. Improved detection times for Mycobacterium avium complex and Mycobacterium tuberculosis with the BACTEC radiometric system. J Clin Microbiol. 1985 Nov;22(5):841–845. doi: 10.1128/jcm.22.5.841-845.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Laszlo A., Eidus L. Test for differentiation of M. tuberculosis and M. bovis from other mycobacteria. Can J Microbiol. 1978 Jun;24(6):754–756. doi: 10.1139/m78-124. [DOI] [PubMed] [Google Scholar]
  4. Laszlo A., Siddiqi S. H. Evaluation of a rapid radiometric differentiation test for the Mycobacterium tuberculosis complex by selective inhibition with p-nitro-alpha-acetylamino-beta-hydroxypropiophenone. J Clin Microbiol. 1984 May;19(5):694–698. doi: 10.1128/jcm.19.5.694-698.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Middlebrook G., Reggiardo Z., Tigertt W. D. Automatable radiometric detection of growth of Mycobacterium tuberculosis in selective media. Am Rev Respir Dis. 1977 Jun;115(6):1066–1069. doi: 10.1164/arrd.1977.115.6.1066. [DOI] [PubMed] [Google Scholar]
  6. Mitchison D. A., Allen B. W., Carrol L., Dickinson J. M., Aber V. R. A selective oleic acid albumin agar medium for tubercle bacilli. J Med Microbiol. 1972 May;5(2):165–175. doi: 10.1099/00222615-5-2-165. [DOI] [PubMed] [Google Scholar]
  7. Morgan M. A., Horstmeier C. D., DeYoung D. R., Roberts G. D. Comparison of a radiometric method (BACTEC) and conventional culture media for recovery of mycobacteria from smear-negative specimens. J Clin Microbiol. 1983 Aug;18(2):384–388. doi: 10.1128/jcm.18.2.384-388.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Park C. H., Hixon D. L., Ferguson C. B., Hall S. L., Risheim C. C., Cook C. B. Rapid recovery of mycobacteria from clinical specimens using automated radiometric technic. Am J Clin Pathol. 1984 Mar;81(3):341–345. doi: 10.1093/ajcp/81.3.341. [DOI] [PubMed] [Google Scholar]
  9. Roberts G. D., Goodman N. L., Heifets L., Larsh H. W., Lindner T. H., McClatchy J. K., McGinnis M. R., Siddiqi S. H., Wright P. Evaluation of the BACTEC radiometric method for recovery of mycobacteria and drug susceptibility testing of Mycobacterium tuberculosis from acid-fast smear-positive specimens. J Clin Microbiol. 1983 Sep;18(3):689–696. doi: 10.1128/jcm.18.3.689-696.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Siddiqi S. H., Hwangbo C. C., Silcox V., Good R. C., Snider D. E., Jr, Middlebrook G. Rapid radiometric methods to detect and differentiate Mycobacterium tuberculosis/M. bovis from other mycobacterial species. Am Rev Respir Dis. 1984 Oct;130(4):634–640. doi: 10.1164/arrd.1984.130.4.634. [DOI] [PubMed] [Google Scholar]
  11. Siddiqi S. H., Libonati J. P., Middlebrook G. Evaluation of rapid radiometric method for drug susceptibility testing of Mycobacterium tuberculosis. J Clin Microbiol. 1981 May;13(5):908–912. doi: 10.1128/jcm.13.5.908-912.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Woods G. L., Washington J. A., 2nd Mycobacteria other than Mycobacterium tuberculosis: review of microbiologic and clinical aspects. Rev Infect Dis. 1987 Mar-Apr;9(2):275–294. doi: 10.1093/clinids/9.2.275. [DOI] [PubMed] [Google Scholar]

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