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. 1990 Jun;28(6):1375–1379. doi: 10.1128/jcm.28.6.1375-1379.1990

Large-scale screening of human sera with cytomegalovirus recombinant antigens.

M P Landini 1, M X Guan 1, G Jahn 1, W Lindenmaier 1, M Mach 1, A Ripalti 1, A Necker 1, T Lazzarotto 1, B Plachter 1
PMCID: PMC267935  PMID: 1696284

Abstract

One of the major problems regarding cytomegalovirus (CMV) serodiagnosis is the use of poorly defined viral antigens. Individual CMV proteins expressed via recombinant DNA procedures are a promising approach to solving this problem. In this work, 10 different fusion proteins containing antigenic epitopes of the major CMV structural proteins of 150, 71, 65, 38, and 28 kilodaltons and of the nonstructural protein of 52 kilodaltons were subjected to Western (immuno-) blotting to assay their reactivities with immunoglobulins G and M in 395 CMV-seropositive and 100 CMV-seronegative unselected human serum samples. As a whole, the results obtained indicate that CMV can be replaced by recombinant viral proteins in the serological evaluation of anti-CMV antibodies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Fleckenstein B., Müller I., Collins J. Cloning of the complete human cytomegalovirus genome in cosmids. Gene. 1982 Apr;18(1):39–46. doi: 10.1016/0378-1119(82)90054-3. [DOI] [PubMed] [Google Scholar]
  2. Hattori M., Sakaki Y. Dideoxy sequencing method using denatured plasmid templates. Anal Biochem. 1986 Feb 1;152(2):232–238. doi: 10.1016/0003-2697(86)90403-3. [DOI] [PubMed] [Google Scholar]
  3. Jahn G., Kouzarides T., Mach M., Scholl B. C., Plachter B., Traupe B., Preddie E., Satchwell S. C., Fleckenstein B., Barrell B. G. Map position and nucleotide sequence of the gene for the large structural phosphoprotein of human cytomegalovirus. J Virol. 1987 May;61(5):1358–1367. doi: 10.1128/jvi.61.5.1358-1367.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Jahn G., Scholl B. C., Traupe B., Fleckenstein B. The two major structural phosphoproteins (pp65 and pp150) of human cytomegalovirus and their antigenic properties. J Gen Virol. 1987 May;68(Pt 5):1327–1337. doi: 10.1099/0022-1317-68-5-1327. [DOI] [PubMed] [Google Scholar]
  5. Klages S., Rüger B., Jahn G. Multiplicity dependent expression of the predominant phosphoprotein pp65 of human cytomegalovirus. Virus Res. 1989 Feb;12(2):159–168. doi: 10.1016/0168-1702(89)90061-0. [DOI] [PubMed] [Google Scholar]
  6. Landini M. P., Lazzarotto T., Ripalti A., Guan M. X., La Placa M. Antibody response to recombinant lambda gt11 fusion proteins in cytomegalovirus infection. J Clin Microbiol. 1989 Oct;27(10):2324–2327. doi: 10.1128/jcm.27.10.2324-2327.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Landini M. P., Michelson S. Human cytomegalovirus proteins. Prog Med Virol. 1988;35:152–185. [PubMed] [Google Scholar]
  8. Landini M. P., Re M. C., Mirolo G., Baldassarri B., La Placa M. Human immune response to cytomegalovirus structural polypeptides studied by immunoblotting. J Med Virol. 1985 Dec;17(4):303–311. doi: 10.1002/jmv.1890170403. [DOI] [PubMed] [Google Scholar]
  9. Mach M., Utz U., Fleckenstein B. Mapping of the major glycoprotein gene of human cytomegalovirus. J Gen Virol. 1986 Jul;67(Pt 7):1461–1467. doi: 10.1099/0022-1317-67-7-1461. [DOI] [PubMed] [Google Scholar]
  10. Meyer H., Bankier A. T., Landini M. P., Brown C. M., Barrell B. G., Rüger B., Mach M. Identification and procaryotic expression of the gene coding for the highly immunogenic 28-kilodalton structural phosphoprotein (pp28) of human cytomegalovirus. J Virol. 1988 Jul;62(7):2243–2250. doi: 10.1128/jvi.62.7.2243-2250.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mocarski E. S., Pereira L., Michael N. Precise localization of genes on large animal virus genomes: use of lambda gt11 and monoclonal antibodies to map the gene for a cytomegalovirus protein family. Proc Natl Acad Sci U S A. 1985 Feb;82(4):1266–1270. doi: 10.1073/pnas.82.4.1266. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Re M. C., Landini M. P., Coppolecchia P., Furlini G., La Placa M. A 28000 molecular weight human cytomegalovirus structural polypeptide studied by means of a specific monoclonal antibody. J Gen Virol. 1985 Nov;66(Pt 11):2507–2511. doi: 10.1099/0022-1317-66-11-2507. [DOI] [PubMed] [Google Scholar]
  13. Ripalti A., Landini M. P., Mocarski E. S., La Placa M. Identification and preliminary use of recombinant lambda gt11 fusion proteins in human cytomegalovirus diagnosis. J Gen Virol. 1989 May;70(Pt 5):1247–1251. doi: 10.1099/0022-1317-70-5-1247. [DOI] [PubMed] [Google Scholar]
  14. Robson L., Gibson W. Primate cytomegalovirus assembly protein: genome location and nucleotide sequence. J Virol. 1989 Feb;63(2):669–676. doi: 10.1128/jvi.63.2.669-676.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rüger B., Klages S., Walla B., Albrecht J., Fleckenstein B., Tomlinson P., Barrell B. Primary structure and transcription of the genes coding for the two virion phosphoproteins pp65 and pp71 of human cytomegalovirus. J Virol. 1987 Feb;61(2):446–453. doi: 10.1128/jvi.61.2.446-453.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Scholl B. C., Von Hintzenstern J., Borisch B., Traupe B., Bröker M., Jahn G. Prokaryotic expression of immunogenic polypeptides of the large phosphoprotein (pp150) of human cytomegalovirus. J Gen Virol. 1988 Jun;69(Pt 6):1195–1204. doi: 10.1099/0022-1317-69-6-1195. [DOI] [PubMed] [Google Scholar]
  17. Stanley K. K., Luzio J. P. Construction of a new family of high efficiency bacterial expression vectors: identification of cDNA clones coding for human liver proteins. EMBO J. 1984 Jun;3(6):1429–1434. doi: 10.1002/j.1460-2075.1984.tb01988.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wingender E., Bercz G., Blöcker H., Frank R., Mayer H. Expression of human parathyroid hormone in Escherichia coli. J Biol Chem. 1989 Mar 15;264(8):4367–4373. [PubMed] [Google Scholar]

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