Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1990 Jun;28(6):1422–1424. doi: 10.1128/jcm.28.6.1422-1424.1990

Evaluation of a new bicolored latex agglutination test for immunological diagnosis of hepatic amoebiasis.

R Robert 1, C Mahaza 1, C Bernard 1, C Buffard 1, J M Senet 1
PMCID: PMC267943  PMID: 2199503

Abstract

A new bicolored latex agglutination amoeba test (BLA) for detection of antibodies against Entamoeba histolytica was evaluated for its practicability and diagnostic sensitivity and specificity. BLA is rapid (5 min) and simple to perform. It requires only 20 microliters of a 1/3-diluted serum, 17 microliters of reagent, and a glass slide. Reading of the test is easy because a positive result shows a green spot with a red surrounding edge. This bicolored pattern is easily distinguishable from the negative test result, which shows a homogeneous dark-brown spot. By using serum samples from 348 individuals, BLA was compared with immunofluorescence assay, indirect hemagglutination, and counterimmunoelectrophoresis. Sensitivity, specificity, efficiency, and positive and negative predictive values of the four methods were almost identical. The results of this study indicate that BLA could be very useful both as a screening method for the diagnosis of invasive amoebiasis and for epidemiological purposes.

Full text

PDF
1422

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Agarwal S. S., Sharma P., Das P., Ahmad J., Dutta G. P. Micro-enzyme linked immunosorbent assay for serodiagnosis of amoebiasis. Indian J Med Res. 1981 Aug;74:219–225. [PubMed] [Google Scholar]
  2. Ambroise-Thomas P., Desgeorges P. T., Monget D. Diagnostic immuno-enzymologique (ELISA) des maladies parasitaires par une microméthode modifiée. 2. Résultats pour la toxoplasmose, l'amibiase, la trichinose, l'hydatidose et l'aspergillose. Bull World Health Organ. 1978;56(5):797–804. [PMC free article] [PubMed] [Google Scholar]
  3. Boonpucknavig S., Nairn R. C. Serological diagnosis of amoebiasis by immunofluorescence. J Clin Pathol. 1967 Nov;20(6):875–878. doi: 10.1136/jcp.20.6.875. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bos H. J., Eijk A. A., Steerenberg P. A. Letter: Application of ELISA--enzyme linked immunosorbent assay--in the sero-diagnosis of amoebiasis. Trans R Soc Trop Med Hyg. 1975;69(4):440–440. doi: 10.1016/0035-9203(75)90202-3. [DOI] [PubMed] [Google Scholar]
  5. Diamond L. S. Techniques of axenic cultivation of Entamoeba histolytica Schaudinn, 1903 and E. histolytica-like amebae. J Parasitol. 1968 Oct;54(5):1047–1056. [PubMed] [Google Scholar]
  6. Gandhi B. M., Irshad M., Chawla T. C., Tandon B. N. Enzyme linked protein-A: an ELISA for detection of amoebic antibody. Trans R Soc Trop Med Hyg. 1987;81(2):183–185. doi: 10.1016/0035-9203(87)90207-0. [DOI] [PubMed] [Google Scholar]
  7. Gentilini M., Pinon J. M., Niel G. Immunoélectrodiffusion sur membrane d'acétate de cellulose. Applications en parasitologie á propos des résultats préliminaires sur 600 essais. Nouv Presse Med. 1972 May 27;1(22):1499–1501. [PubMed] [Google Scholar]
  8. Hadfield S. G., Lane A., McIllmurray M. B. A novel coloured latex test for the detection and identification of more than one antigen. J Immunol Methods. 1987 Mar 12;97(2):153–158. doi: 10.1016/0022-1759(87)90454-6. [DOI] [PubMed] [Google Scholar]
  9. Hartmann D. P., Ghadirian E., Meerovitch E. Enzyme-linked immunosorbent assay (ELISA) and indirect hemagglutination (IHA) test in the serodiagnosis of experimental hepatic amebiasis. J Parasitol. 1980 Apr;66(2):344–345. [PubMed] [Google Scholar]
  10. Jeanes A. L. Indirect fluorescent antibody test in diagnosis of hepatic amoebiasis. Br Med J. 1966 Jun 11;1(5501):1464–1464. doi: 10.1136/bmj.1.5501.1464. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kozinn P. J., Taschdjian C. L., Goldberg P. K., Protzmann W. P., MacKenzie D. W., Remington J. S., Anderson S., Seelig M. S. Efficiency of serologic tests in the diagnosis of systemic candidiasis. Am J Clin Pathol. 1978 Dec;70(6):893–898. doi: 10.1093/ajcp/70.6.893. [DOI] [PubMed] [Google Scholar]
  12. Krupp I. M. Comparison of counterimmunoelectrophoresis with other serologic tests in the diagnosis of amebiasis. Am J Trop Med Hyg. 1974 Jan;23(1):27–30. doi: 10.4269/ajtmh.1974.23.27. [DOI] [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. Morris M. N., Powell S. J., Elsdon-Dew R. Latex agglutination test for invasive amoebiasis. Lancet. 1970 Jun 27;1(7661):1362–1363. doi: 10.1016/s0140-6736(70)91270-5. [DOI] [PubMed] [Google Scholar]
  15. Osisanya J. O., Warhurst D. C. Specific anti-amoebic immunoglobulins and the cellulose acetate precipitin test in Entamoeba histolytica infection. Trans R Soc Trop Med Hyg. 1980;74(5):605–608. doi: 10.1016/0035-9203(80)90149-2. [DOI] [PubMed] [Google Scholar]
  16. Pahuchonma W., Janitschke K. Serodiagnosis of parasitic infections by ELISA with different antigens. Southeast Asian J Trop Med Public Health. 1987 Jun;18(2):193–196. [PubMed] [Google Scholar]
  17. Pinon J. M., Poirriez J., Remy G., Lepan H. Immunological studies in amebiasis: isotypic characterization of specific antibodies by enzyme-linked immunofiltration assay. Am J Trop Med Hyg. 1987 Sep;37(2):290–295. doi: 10.4269/ajtmh.1987.37.290. [DOI] [PubMed] [Google Scholar]
  18. Prakash O., Tandon B. N., Bhalla I., Ay A. K., Vinayak V. K. Indirect hemagglutination and ameba-immobilization tests and their evaluation in intestinal and extraintestinal amebiasis. Am J Trop Med Hyg. 1969 Sep;18(5):670–675. doi: 10.4269/ajtmh.1969.18.670. [DOI] [PubMed] [Google Scholar]
  19. Sathar M. A., Simjee A. E., Nel J. D., Bredenkamp B. L., Gathiram V., Jackson T. F. Evaluation of an enzyme-linked immunosorbent assay in the serodiagnosis of amoebic liver abscess. S Afr Med J. 1988 Dec 17;74(12):625–628. [PubMed] [Google Scholar]
  20. Sharma M., Saxena A., Ghosh S., Samantaray J. C., Talwar G. P. A simple & rapid Dot-ELISA dipstick technique for detection of antibodies to Entamoeba histolytica in amoebic liver abscess. Indian J Med Res. 1988 Nov;88:409–415. [PubMed] [Google Scholar]
  21. Tandon A. Use of enzyme linked immunosorbent assay in intestinal and extra-intestinal amoebiasis (amoebic liver abscess). Trans R Soc Trop Med Hyg. 1981;75(4):574–575. doi: 10.1016/0035-9203(81)90204-2. [DOI] [PubMed] [Google Scholar]
  22. Voller A., Bidwell D. E., Bartlett A., Edwards R. A comparison of isotopic and enzyme-immunoassays for tropical parasitic diseases. Trans R Soc Trop Med Hyg. 1977;71(5):431–437. doi: 10.1016/0035-9203(77)90046-3. [DOI] [PubMed] [Google Scholar]
  23. Yang J., Kennedy M. T. Evaluation of enzyme-linked immunosorbent assay for the serodiagnosis of amebiasis. J Clin Microbiol. 1979 Dec;10(6):778–785. doi: 10.1128/jcm.10.6.778-785.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES