Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1990 Jun;28(6):1447–1448. doi: 10.1128/jcm.28.6.1447-1448.1990

Comparison of IDEIA III and cell culture for the detection of Chlamydia trachomatis in endocervical specimens.

W D Lebar 1, H Schubiner 1, C Jemal 1, B R Herschman 1
PMCID: PMC267952  PMID: 2199508

Abstract

A total of 803 endocervical samples were obtained from females with clinical or epidemiological histories suggesting chlamydia infection. These specimens were tested by IDEIA III and cell culture for the presence of Chlamydia trachomatis. After resolution of discrepant results by direct fluorescent-antibody staining of pelleted cell culture transport materials, IDEIA III demonstrated sensitivity, specificity, and positive and negative predictive values of 93.8, 99, 92.9, and 99.1%, respectively.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Caul E. O., Paul I. D. Monoclonal antibody based ELISA for detecting Chlamydia trachomatis. Lancet. 1985 Feb 2;1(8423):279–279. doi: 10.1016/s0140-6736(85)91056-6. [DOI] [PubMed] [Google Scholar]
  2. Grillner L., Beckman S., Hammar H. Comparison of two enzyme immunoassays and an immunofluorescence test for detection of Chlamydia trachomatis. Eur J Clin Microbiol. 1986 Oct;5(5):559–562. doi: 10.1007/BF02017705. [DOI] [PubMed] [Google Scholar]
  3. LeBar W., Herschman B., Jemal C., Pierzchala J. Comparison of DNA probe, monoclonal antibody enzyme immunoassay, and cell culture for the detection of Chlamydia trachomatis. J Clin Microbiol. 1989 May;27(5):826–828. doi: 10.1128/jcm.27.5.826-828.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Mahony J., Castriciano S., Sellors J., Stewart I., Cunningham I., Landis S., Seidelman W., Grant L., Devlin C., Chernesky M. Diagnosis of Chlamydia trachomatis genital infections by cell culture and two enzyme immunoassays detecting different chlamydial antigens. J Clin Microbiol. 1989 Sep;27(9):1934–1938. doi: 10.1128/jcm.27.9.1934-1938.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Mohanty K. C., O'Neill J. J., Hambling M. H. Comparison of enzyme immunoassays and cell culture for detecting Chlamydia trachomatis. Genitourin Med. 1986 Jun;62(3):175–176. doi: 10.1136/sti.62.3.175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Pugh S. F., Slack R. C., Caul E. O., Paul I. D., Appleton P. N., Gatley S. Enzyme amplified immunoassay: a novel technique applied to direct detection of Chlamydia trachomatis in clinical specimens. J Clin Pathol. 1985 Oct;38(10):1139–1141. doi: 10.1136/jcp.38.10.1139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Sulaiman M. Z., Foster J., Pugh S. F. Prevalence of Chlamydia trachomatis infection in homosexual men. Genitourin Med. 1987 Jun;63(3):179–181. doi: 10.1136/sti.63.3.179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Taylor-Robinson D., Tuffrey M. Comparison of detection procedures for Chlamydia trachomatis, including enzyme immunoassays, in a mouse model of genital infection. J Med Microbiol. 1987 Sep;24(2):169–173. doi: 10.1099/00222615-24-2-169. [DOI] [PubMed] [Google Scholar]
  9. Thomas B. J., Osborn M. F., Gilchrist C., Taylor-Robinson D. Improved sensitivity of an enzyme immunoassay IDEIA for detecting Chlamydia trachomatis. J Clin Pathol. 1989 Jul;42(7):759–762. doi: 10.1136/jcp.42.7.759. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Tjiam K. H., van Heijst B. Y., van Zuuren A., Wagenvoort J. H., van Joost T., Stolz E., Michel M. F. Evaluation of an enzyme immunoassay for the diagnosis of chlamydial infections in urogenital specimens. J Clin Microbiol. 1986 Apr;23(4):752–754. doi: 10.1128/jcm.23.4.752-754.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Weismeier E., Bruckner D. A., Malotte C. K., Manduke L. Enzyme-linked immunoabsorbent assays in the detection of Chlamydia trachomatis: how valid are they? Diagn Microbiol Infect Dis. 1988 Apr;9(4):219–223. doi: 10.1016/0732-8893(88)90112-5. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES