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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1990 Jun;28(6):1465–1468. doi: 10.1128/jcm.28.6.1465-1468.1990

Identification of enterotoxigenic Escherichia coli by colony hybridization with nonradioactive digoxigenin-labeled DNA probes.

L K Riley 1, C J Caffrey 1
PMCID: PMC267959  PMID: 2199513

Abstract

Enterotoxigenic Escherichia coli (ETEC) strains were readily identified in pure and mixed cultures with nonradioactive, digoxigenin-labeled DNA probes coding for heat-labile (LTI) and heat-stable (STaI, STaII, and STb) enterotoxins. Digoxigenin-labeled ETEC fragments were more sensitive than and exhibited less nonspecific background contamination than biotinylated ETEC probes.

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Selected References

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