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. 1990 Jun;28(6):1477–1478. doi: 10.1128/jcm.28.6.1477-1478.1990

Evaluation of MicroScan for identification of Enterococcus species.

D M Tritz 1, P C Iwen 1, G L Woods 1
PMCID: PMC267962  PMID: 2116449

Abstract

Emerging drug resistance of the enterococci necessitates differentiation from group D streptococci and accurate species identification. MicroScan (Baxter Healthcare Corp., West Sacramento, Calif.) has recently developed a microdilution system for identification and antibiotic susceptibility testing of gram-positive cocci. To evaluate the ability of this system to identify Enterococcus species, 100 isolate identified as enterococci by MicroScan were tested by conventional media and 60 isolates of streptococci were tested by MicroScan. Incubation times for conventional and MicroScan methods were 96 and 18 to 24 h, respectively. For 94 strains of enterococci (77 Enterococcus faecalis, 14 Enterococcus faecium, and 3 Enterococcus durans), identification by conventional media and MicroScan agreed. Of the remaining six isolates, four were identified as E. faecalis and two were identified as E. durans by MicroScan, whereas by conventional media the four E. faecalis isolates were identified as Enterococcus solitarius and the two E. durans isolates were identified as Enterococcus hirae. None of the 60 streptococci were identified as enterococci. MicroScan is a reliable method for identification of the commonly encountered enterococcal species E. faecalis and E. faecium; however, modifications of the system are necessary for identification of other Enterococcus species.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bosley G. S., Facklam R. R., Grossman D. Rapid identification of enterococci. J Clin Microbiol. 1983 Nov;18(5):1275–1277. doi: 10.1128/jcm.18.5.1275-1277.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brown L. H., Peterson E. M., de la Maza L. M. Rapid identification of enterococci. J Clin Microbiol. 1983 Feb;17(2):369–370. doi: 10.1128/jcm.17.2.369-370.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Facklam R. R., Collins M. D. Identification of Enterococcus species isolated from human infections by a conventional test scheme. J Clin Microbiol. 1989 Apr;27(4):731–734. doi: 10.1128/jcm.27.4.731-734.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hamoudi A. C., Marcon M. J., Cannon H. J. Evaluation of rapid identification of gram-positive cocci in positive blood cultures by use of the AutoMicrobic system Gram-Positive Identification Card. J Clin Microbiol. 1984 Aug;20(2):171–174. doi: 10.1128/jcm.20.2.171-174.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jorgensen J. H., Crawford S. A., Alexander G. A. Rapid identification of group D streptococci with the API 20S system. J Clin Microbiol. 1983 Jun;17(6):1096–1098. doi: 10.1128/jcm.17.6.1096-1098.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kaplan A. H., Gilligan P. H., Facklam R. R. Recovery of resistant enterococci during vancomycin prophylaxis. J Clin Microbiol. 1988 Jun;26(6):1216–1218. doi: 10.1128/jcm.26.6.1216-1218.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kaye D. Enterococci. Biologic and epidemiologic characteristics and in vitro susceptibility. Arch Intern Med. 1982 Oct 25;142(11):2006–2009. doi: 10.1001/archinte.142.11.2006. [DOI] [PubMed] [Google Scholar]
  8. Kim M. J., Weiser M., Gottschall S., Randall E. L. Identification of Streptococcus faecalis and Streptococcus faecium and susceptibility studies with newly developed antimicrobial agents. J Clin Microbiol. 1987 May;25(5):787–790. doi: 10.1128/jcm.25.5.787-790.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Murray B. E. The life and times of the Enterococcus. Clin Microbiol Rev. 1990 Jan;3(1):46–65. doi: 10.1128/cmr.3.1.46. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Parker M. T., Ball L. C. Streptococci and aerococci associated with systemic infection in man. J Med Microbiol. 1976 Aug;9(3):275–302. doi: 10.1099/00222615-9-3-275. [DOI] [PubMed] [Google Scholar]
  11. Patterson J. E., Colodny S. M., Zervos M. J. Serious infection due to beta-lactamase-producing Streptococcus faecalis with high-level resistance to gentamicin. J Infect Dis. 1988 Nov;158(5):1144–1145. doi: 10.1093/infdis/158.5.1144. [DOI] [PubMed] [Google Scholar]
  12. Sapico F. L., Canawati H. N., Ginunas V. J., Gilmore D. S., Montgomerie J. Z., Tuddenham W. J., Facklam R. R. Enterococci highly resistant to penicillin and ampicillin: an emerging clinical problem? J Clin Microbiol. 1989 Sep;27(9):2091–2095. doi: 10.1128/jcm.27.9.2091-2095.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Spiegel C. A. Laboratory detection of high-level aminoglycoside-aminocyclitol resistance in Enterococcus spp. J Clin Microbiol. 1988 Nov;26(11):2270–2274. doi: 10.1128/jcm.26.11.2270-2274.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Terpenning M. S., Zervos M. J., Schaberg D. R., Kauffman C. A. Enterococcal infections: an increasing problem in hospitalized patients. Infect Control Hosp Epidemiol. 1988 Oct;9(10):457–461. doi: 10.1086/645743. [DOI] [PubMed] [Google Scholar]
  15. Tofte R. W., Solliday J. A., Crossley K. B. Susceptibilities of enterococci to twelve antibiotics. Antimicrob Agents Chemother. 1984 Apr;25(4):532–533. doi: 10.1128/aac.25.4.532. [DOI] [PMC free article] [PubMed] [Google Scholar]

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