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. 1990 Jul;28(7):1669–1670. doi: 10.1128/jcm.28.7.1669-1670.1990

Enhancing effect of centrifugation on isolation of influenza virus from clinical specimens.

M Seno 1, Y Kanamoto 1, S Takao 1, N Takei 1, S Fukuda 1, H Umisa 1
PMCID: PMC268014  PMID: 2380390

Abstract

The use of centrifugation (700 x g, 60 min) in a plaque assay markedly increased (mean, 2.9-fold) the infectivity of all 42 influenza virus strains tested, compared with no centrifugation. Of 13 influenza virus strains isolated from 390 clinical specimens, 9 (69%) were efficiently isolated by the centrifugation assay compared with conventional culture methods. The centrifugation assay may be useful for isolating the influenza virus from clinical specimens.

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Selected References

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  1. Chomel J. J., Thouvenot D., Onno M., Kaiser C., Gourreau J. M., Aymard M. Rapid diagnosis of influenza infection of NP antigen using an immunocapture ELISA test. J Virol Methods. 1989 Jul;25(1):81–91. doi: 10.1016/0166-0934(89)90102-x. [DOI] [PubMed] [Google Scholar]
  2. Espy M. J., Smith T. F., Harmon M. W., Kendal A. P. Rapid detection of influenza virus by shell vial assay with monoclonal antibodies. J Clin Microbiol. 1986 Oct;24(4):677–679. doi: 10.1128/jcm.24.4.677-679.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gleaves C. A., Smith T. F., Shuster E. A., Pearson G. R. Rapid detection of cytomegalovirus in MRC-5 cells inoculated with urine specimens by using low-speed centrifugation and monoclonal antibody to an early antigen. J Clin Microbiol. 1984 Jun;19(6):917–919. doi: 10.1128/jcm.19.6.917-919.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gleaves C. A., Wilson D. J., Wold A. D., Smith T. F. Detection and serotyping of herpes simplex virus in MRC-5 cells by use of centrifugation and monoclonal antibodies 16 h postinoculation. J Clin Microbiol. 1985 Jan;21(1):29–32. doi: 10.1128/jcm.21.1.29-32.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Nikkari S., Halonen P., Kharitonenkov I., Kivivirta M., Khristova M., Waris M., Kendal A. One-incubation time-resolved fluoroimmunoassay based on monoclonal antibodies in detection of influenza A and B viruses directly in clinical specimens. J Virol Methods. 1989 Jan;23(1):29–40. doi: 10.1016/0166-0934(89)90086-4. [DOI] [PubMed] [Google Scholar]
  6. Pachucki C. T., Creticos C. Early detection of influenza virus by using a fluorometric assay of infected tissue culture. J Clin Microbiol. 1988 Dec;26(12):2664–2666. doi: 10.1128/jcm.26.12.2664-2666.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Salomón H. E., Grandien M., Avila M. M., Pettersson C. A., Weissenbacher M. C. Comparison of three techniques for detection of respiratory viruses in nasopharyngeal aspirates from children with lower acute respiratory infections. J Med Virol. 1989 Jul;28(3):159–162. doi: 10.1002/jmv.1890280310. [DOI] [PubMed] [Google Scholar]
  8. Stokes C. E., Bernstein J. M., Kyger S. A., Hayden F. G. Rapid diagnosis of influenza A and B by 24-h fluorescent focus assays. J Clin Microbiol. 1988 Jul;26(7):1263–1266. doi: 10.1128/jcm.26.7.1263-1266.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Tenser R. B., Dunstan M. E. Mechanisms of herpes simplex virus infectivity enhanced by ultracentrifugal inoculation. Infect Immun. 1980 Oct;30(1):193–197. doi: 10.1128/iai.30.1.193-197.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Tenser R. B. Ultracentrifugal inoculation of herpes simplex virus. Infect Immun. 1978 Jul;21(1):281–285. doi: 10.1128/iai.21.1.281-285.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Tobita K. Permanent canine kidney (MDCK) cells for isolation and plaque assay of influenza B viruses. Med Microbiol Immunol. 1975 Dec 30;162(1):23–27. doi: 10.1007/BF02123574. [DOI] [PubMed] [Google Scholar]
  12. Tobita K., Sugiura A., Enomote C., Furuyama M. Plaque assay and primary isolation of influenza A viruses in an established line of canine kidney cells (MDCK) in the presence of trypsin. Med Microbiol Immunol. 1975 Dec 30;162(1):9–14. doi: 10.1007/BF02123572. [DOI] [PubMed] [Google Scholar]
  13. Walls H. H., Harmon M. W., Slagle J. J., Stocksdale C., Kendal A. P. Characterization and evaluation of monoclonal antibodies developed for typing influenza A and influenza B viruses. J Clin Microbiol. 1986 Feb;23(2):240–245. doi: 10.1128/jcm.23.2.240-245.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Woods G. L., Johnson A. M. Rapid 24-well plate centrifugation assay for detection of influenza A virus in clinical specimens. J Virol Methods. 1989 Apr-May;24(1-2):35–42. doi: 10.1016/0166-0934(89)90005-0. [DOI] [PubMed] [Google Scholar]

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