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. 1990 Jul;28(7):1671–1672. doi: 10.1128/jcm.28.7.1671-1672.1990

Buffered charcoal-yeast extract medium for the isolation of brucellae.

I Raad 1, K Rand 1, D Gaskins 1
PMCID: PMC268015  PMID: 2116452

Abstract

A patient with chronic osteomyelitis caused by Brucella abortus had negative agglutination titers. Because of a superimposed staphylococcal infection that resulted in the overgrowth of this organism on nonselective media, brucellae were isolated only on a selective buffered charcoal-yeast extract (BCYE) agar. Sixteen strains of various Brucella species were inoculated on BCYE agar; selective BCYE agar with polymyxin, anisomycin, and cefamandole; and brucella blood agar. The growth and recovery rates on the three media tested were comparable for 14 strains. BCYE agar with polymyxin, anisomycin, and cefamandole may be useful as a selective medium for the isolation of brucellae.

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Selected References

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