Abstract
Five murine monoclonal antibodies (MAbs) against the NADL strain of bovine viral diarrhea (BVD) virus were developed, identified, and characterized. Four of the MAbs were directed against a 53-kilodalton (kDa) viral protein, and one was specific to a 47-kDa polypeptide. Competitive radioimmunoassay showed that two MAbs were specific to related epitopes of the 53-kDa protein, and the other three MAbs were each specific to a different epitope. The MAbs were used to study heterogeneity among BVD virus strains. Various degrees of reactivity of cytopathic and noncytopathic virus isolates were detected by virus neutralization and immunofluorescence assays. The virus isolates were divided into six groups based on the neutralization test. The results indicated that the 53-kDa glycoprotein of BVD virus is the major protein involved in virus neutralization and that only a few epitopes of the protein contribute to the neutralization. None of the MAbs neutralized all the BVD virus isolates tested in this study, suggesting antigenic variations among BVD virus isolates.
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Selected References
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- Baker J. C. Bovine viral diarrhea virus: a review. J Am Vet Med Assoc. 1987 Jun 1;190(11):1449–1458. [PubMed] [Google Scholar]
- Bolin S. R. Viral and viral protein specificity of antibodies induced in cows persistently infected with noncytopathic bovine viral diarrhea virus after vaccination with cytopathic bovine viral diarrhea virus. Am J Vet Res. 1988 Jul;49(7):1040–1044. [PubMed] [Google Scholar]
- Bolin S., Moennig V., Kelso Gourley N. E., Ridpath J. Monoclonal antibodies with neutralizing activity segregate isolates of bovine viral diarrhea virus into groups. Brief report. Arch Virol. 1988;99(1-2):117–123. doi: 10.1007/BF01311029. [DOI] [PubMed] [Google Scholar]
- Collett M. S., Anderson D. K., Retzel E. Comparisons of the pestivirus bovine viral diarrhoea virus with members of the flaviviridae. J Gen Virol. 1988 Oct;69(Pt 10):2637–2643. doi: 10.1099/0022-1317-69-10-2637. [DOI] [PubMed] [Google Scholar]
- Corapi W. V., Donis R. O., Dubovi E. J. Monoclonal antibody analyses of cytopathic and noncytopathic viruses from fatal bovine viral diarrhea virus infections. J Virol. 1988 Aug;62(8):2823–2827. doi: 10.1128/jvi.62.8.2823-2827.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Coria M. F., Schmerr M. J., McClurkin A. W. Characterization of the major structural proteins of purified bovine viral diarrhea virus. Arch Virol. 1983;76(4):335–339. doi: 10.1007/BF01311200. [DOI] [PubMed] [Google Scholar]
- Donis R. O., Corapi W., Dubovi E. J. Neutralizing monoclonal antibodies to bovine viral diarrhoea virus bind to the 56K to 58K glycoprotein. J Gen Virol. 1988 Jan;69(Pt 1):77–86. doi: 10.1099/0022-1317-69-1-77. [DOI] [PubMed] [Google Scholar]
- Donis R. O., Dubovi E. J. Characterization of bovine viral diarrhoea-mucosal disease virus-specific proteins in bovine cells. J Gen Virol. 1987 Jun;68(Pt 6):1597–1605. doi: 10.1099/0022-1317-68-6-1597. [DOI] [PubMed] [Google Scholar]
- Donis R. O., Dubovi E. J. Differences in virus-induced polypeptides in cells infected by cytopathic and noncytopathic biotypes of bovine virus diarrhea-mucosal disease virus. Virology. 1987 May;158(1):168–173. doi: 10.1016/0042-6822(87)90250-9. [DOI] [PubMed] [Google Scholar]
- Donis R. O., Dubovi E. J. Glycoproteins of bovine viral diarrhoea-mucosal disease virus in infected bovine cells. J Gen Virol. 1987 Jun;68(Pt 6):1607–1616. doi: 10.1099/0022-1317-68-6-1607. [DOI] [PubMed] [Google Scholar]
- Edwards S., Sands J. J., Harkness J. W. The application of monoclonal antibody panels to characterize pestivirus isolates from ruminants in Great Britain. Arch Virol. 1988;102(3-4):197–206. doi: 10.1007/BF01310825. [DOI] [PubMed] [Google Scholar]
- Hafez S. M., Liess B., Frey H. R. Studies on the natural occurrence of neutralizing antibodies against six strains of bovine viral diarrhea virus. Zentralbl Veterinarmed B. 1976 Sep;23(8):669–677. doi: 10.1111/j.1439-0450.1976.tb00705.x. [DOI] [PubMed] [Google Scholar]
- Howard C. J., Brownlie J., Clarke M. C. Comparison by the neutralisation assay of pairs of non-cytopathogenic and cytopathogenic strains of bovine virus diarrhoea virus isolated from cases of mucosal disease. Vet Microbiol. 1987 Apr;13(4):361–369. doi: 10.1016/0378-1135(87)90067-8. [DOI] [PubMed] [Google Scholar]
- Kimura-Kuroda J., Yasui K. Topographical analysis of antigenic determinants on envelope glycoprotein V3 (E) of Japanese encephalitis virus, using monoclonal antibodies. J Virol. 1983 Jan;45(1):124–132. doi: 10.1128/jvi.45.1.124-132.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Magar R., Minocha H. C., Lecomte J. Bovine viral diarrhea virus proteins: heterogeneity of cytopathogenic and non-cytopathogenic strains and evidence of a 53K glycoprotein neutralization epitope. Vet Microbiol. 1988 Apr;16(4):303–314. doi: 10.1016/0378-1135(88)90012-0. [DOI] [PubMed] [Google Scholar]
- Magar R., Minocha H. C., Montpetit C., Carman P. S., Lecomte J. Typing of cytopathic and noncytopathic bovine viral diarrhea virus reference and Canadian field strains using a neutralizing monoclonal antibody. Can J Vet Res. 1988 Jan;52(1):42–45. [PMC free article] [PubMed] [Google Scholar]
- Markwell M. A. A new solid-state reagent to iodinate proteins. I. Conditions for the efficient labeling of antiserum. Anal Biochem. 1982 Sep 15;125(2):427–432. doi: 10.1016/0003-2697(82)90025-2. [DOI] [PubMed] [Google Scholar]
- Matthaeus W. Detection of three polypeptides in preparations of bovine viral diarrhoea virus. Arch Virol. 1979;59(4):299–305. doi: 10.1007/BF01317470. [DOI] [PubMed] [Google Scholar]
- Nettleton P. F. Pathogenesis and epidemiology of border disease. Ann Rech Vet. 1987;18(2):147–155. [PubMed] [Google Scholar]
- Peters W., Greiser-Wilke I., Moennig V., Liess B. Preliminary serological characterization of bovine viral diarrhoea virus strains using monoclonal antibodies. Vet Microbiol. 1986 Sep;12(3):195–200. doi: 10.1016/0378-1135(86)90048-9. [DOI] [PubMed] [Google Scholar]
- Pocock D. H., Howard C. J., Clarke M. C., Brownlie J. Variation in the intracellular polypeptide profiles from different isolates of bovine virus diarrhoea virus. Arch Virol. 1987;94(1-2):43–53. doi: 10.1007/BF01313724. [DOI] [PubMed] [Google Scholar]
- Purchio A. F., Larson R., Collett M. S. Characterization of bovine viral diarrhea virus proteins. J Virol. 1984 May;50(2):666–669. doi: 10.1128/jvi.50.2.666-669.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Radostits O. M., Littlejohns I. R. New concepts in the pathogenesis, diagnosis and control of diseases caused by the bovine viral diarrhea virus. Can Vet J. 1988 Jun;29(6):513–528. [PMC free article] [PubMed] [Google Scholar]
- Renard A., Schmetz D., Guiot C., Brown-Shimmer S., Dagenais L., Pastoret P. P., Dina D., Martial J. A. Molecular cloning of the bovine viral diarrhea virus genomic RNA. Ann Rech Vet. 1987;18(2):121–125. [PubMed] [Google Scholar]
- Sprecher D. J., Leman A. D., Carlisle S. Effects of parasympathominetics on porcine stillbirth. Am J Vet Res. 1975 Sep;36(9):1331–1333. [PubMed] [Google Scholar]