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. 1990 Aug;28(8):1739–1743. doi: 10.1128/jcm.28.8.1739-1743.1990

Detection of specific immunoglobulin E in patients with toxoplasmosis.

J M Pinon 1, D Toubas 1, C Marx 1, G Mougeot 1, A Bonnin 1, A Bonhomme 1, M Villaume 1, F Foudrinier 1, H Lepan 1
PMCID: PMC268039  PMID: 2203811

Abstract

An immunocapture assay was developed to detect Toxoplasma gondii-specific immunoglobulin E (IgE) in sera from adults with acute acquired infection or reactivation and from babies with congenital toxoplasmosis. The components of this assay were monoclonal antibody to human IgE, samples from patients, and T. gondii tachyzoites treated with Formalin. When T. gondii-specific IgE antibodies were present, visually detectable agglutination occurred. Sera, umbilical cord blood, fetal blood, cerebrospinal fluid, and amniotic fluid were tested by this method. Specific IgE antibodies were detected in sera from 25 (86%) of 29 adults who developed specific IgG antibody during pregnancy or had specific IgA and IgM antibodies. Specific IgE was present early during infection, at the time that IgM antibodies were present, and slightly preceding the presence of specific IgA antibodies. In 23 patients tested serially, IgE antibodies never persisted for longer than 4 months. No nonspecific anti-T. gondii IgE was detected in sera from uninfected individuals. Maternal IgE antibodies did not cross the placenta. In sera of patients with congenital toxoplasmosis, specific IgE antibodies were found at birth, during the first year of life, and during immunologic recrudescence following discontinuation of pyrimethamine-sulfonamide therapy. The IgE immunocapture assay is simple to perform. It is especially useful for determining when T. gondii was acquired by recently infected pregnant women.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Derouin F., Sulcebe G., Ballet J. J. Sequential determination of IgG subclasses and IgA specific antibodies in primary and reactivating toxoplasmosis. Biomed Pharmacother. 1987;41(8):429–433. [PubMed] [Google Scholar]
  2. Desmonts G., Naot Y., Remington J. S. Immunoglobulin M-immunosorbent agglutination assay for diagnosis of infectious diseases: diagnosis of acute congenital and acquired Toxoplasma infections. J Clin Microbiol. 1981 Nov;14(5):486–491. doi: 10.1128/jcm.14.5.486-491.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Desmonts G., Remington J. S. Direct agglutination test for diagnosis of Toxoplasma infection: method for increasing sensitivity and specificity. J Clin Microbiol. 1980 Jun;11(6):562–568. doi: 10.1128/jcm.11.6.562-568.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dutton G. N., Hay J. Toxoplasmic retinochoroiditis--current concepts in pathogenesis. Trans Ophthalmol Soc U K. 1983;103(Pt 5):503–507. [PubMed] [Google Scholar]
  5. Dutton G. N. The causes of tissue damage in toxoplasmic retinochoroiditis. Trans Ophthalmol Soc U K. 1986;105(Pt 4):404–412. [PubMed] [Google Scholar]
  6. Frenkel J. K., Escajadillo A. Cyst rupture as a pathogenic mechanism of toxoplasmic encephalitis. Am J Trop Med Hyg. 1987 May;36(3):517–522. doi: 10.4269/ajtmh.1987.36.517. [DOI] [PubMed] [Google Scholar]
  7. Geller M., Geller M., Flaherty D. K., Capanema de Sourza A. P. Serum IgE levels in toxoplasmosis. Ann Allergy. 1980 Oct;45(4):251–252. [PubMed] [Google Scholar]
  8. Israelski D. M., Remington J. S. Toxoplasmic encephalitis in patients with AIDS. Infect Dis Clin North Am. 1988 Jun;2(2):429–445. [PubMed] [Google Scholar]
  9. Naot Y., Barnett E. V., Remington J. S. Method for avoiding false-positive results occurring in immunoglobulin M enzyme-linked immunosorbent assays due to presence of both rheumatoid factor and antinuclear antibodies. J Clin Microbiol. 1981 Jul;14(1):73–78. doi: 10.1128/jcm.14.1.73-78.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Newman P. E., Ghosheh R., Tabbara K. F., O'Connor G. R., Stern W. The role of hypersensitivity reactions to toxoplasma antigens in experimental ocular toxoplasmosis in nonhuman primates. Am J Ophthalmol. 1982 Aug;94(2):159–164. doi: 10.1016/0002-9394(82)90070-8. [DOI] [PubMed] [Google Scholar]
  11. Pinon J. M., Poirriez J., Leroux B., Dupouy D., Quereux C., Garin J. P. Diagnostic précoce et surveillance de la toxoplasmose congénitale. Méthode des profils immunologiques comparés. Presse Med. 1987 Mar 21;16(10):471–474. [PubMed] [Google Scholar]
  12. Pinon J. M., Thoannes H., Gruson N. An enzyme-linked immuno-filtration assay used to compare infant and maternal antibody profiles in toxoplasmosis. J Immunol Methods. 1985 Feb 28;77(1):15–23. doi: 10.1016/0022-1759(85)90179-6. [DOI] [PubMed] [Google Scholar]
  13. Pinon J. M., Thoannes H., Pouletty P. H., Poirriez J., Damiens J., Pelletier P. Detection of IgA specific for toxoplasmosis in serum and cerebrospinal fluid using a non-enzymatic IgA-capture assay. Diagn Immunol. 1986;4(5):223–227. [PubMed] [Google Scholar]
  14. Poirriez J., Toubas D., Marx-Chemla C., Leroux B., Dupouy D., Talmud M., Pinon J. M. Isotypic characterization of anti-Toxoplasma gondii antibodies in 18 cases of congenital toxoplasmic chorioretinitis. Acta Ophthalmol (Copenh) 1989 Apr;67(2):164–168. doi: 10.1111/j.1755-3768.1989.tb00747.x. [DOI] [PubMed] [Google Scholar]
  15. Pouletty P., Kadouche J., Garcia-Gonzalez M., Mihaesco E., Desmonts G., Thulliez P., Thoannes H., Pinon J. M. An anti-human mu chain monoclonal antibody: use for detection of IgM antibodies to Toxoplasma gondii by reverse immunosorbent assay. J Immunol Methods. 1985 Feb 11;76(2):289–298. doi: 10.1016/0022-1759(85)90306-0. [DOI] [PubMed] [Google Scholar]
  16. Remington J. S., Miller M. J., Brownlee I. IgM antibodies in acute toxoplasmosis. II. Prevalence and significance in acquired cases. J Lab Clin Med. 1968 May;71(5):855–866. [PubMed] [Google Scholar]
  17. Ridel P. R., Auriault C., Darcy F., Pierce R. J., Leite P., Santoro F., Neyrinck J. L., Kusnierz J. P., Capron A. Protective role of IgE in immunocompromised rat toxoplasmosis. J Immunol. 1988 Aug 1;141(3):978–983. [PubMed] [Google Scholar]
  18. Wakefield D., Penny R. Immunology of ocular toxoplasmosis. Aust J Ophthalmol. 1982 Nov;10(4):277–281. doi: 10.1111/j.1442-9071.1982.tb01630.x. [DOI] [PubMed] [Google Scholar]
  19. Wyler D. J., Blackman H. J., Lunde M. N. Cellular hypersensitivity to toxoplasmal and retinal antigens in patients with toxoplasmal retinochoroiditis. Am J Trop Med Hyg. 1980 Nov;29(6):1181–1186. doi: 10.4269/ajtmh.1980.29.1181. [DOI] [PubMed] [Google Scholar]
  20. van Loon A. M., van der Logt J. T., Heessen F. W., van der Veen J. Quantitation of immunoglobulin E antibody to cytomegalovirus by antibody capture enzyme-linked immunosorbent assay. J Clin Microbiol. 1985 Apr;21(4):558–561. doi: 10.1128/jcm.21.4.558-561.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]

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