Abstract
Five DNA probes directed against different regions of the gene that encodes the dermonecrotic toxin of Pasteurella multocida subsp. multocida were examined for their ability to identify toxigenic P. multocida subsp. multocida strains. The specificities of the probes were studied with 96 strains of P. multocida subsp. multocida and 22 strains of 11 other bacterial species. Results of colony hybridization assays using these probes indicated that two of the five probes have potential diagnostic value.
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- Buys W. E., Smith H. E., Kamps A. M., Kamp E. M., Smits M. A. Sequence of the dermonecrotic toxin of Pasteurella multocida ssp. multocida. Nucleic Acids Res. 1990 May 11;18(9):2815–2816. doi: 10.1093/nar/18.9.2815. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Foged N. T., Nielsen J. P., Pedersen K. B. Differentiation of toxigenic from nontoxigenic isolates of Pasteurella multocida by enzyme-linked immunosorbent assay. J Clin Microbiol. 1988 Jul;26(7):1419–1420. doi: 10.1128/jcm.26.7.1419-1420.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hanahan D., Meselson M. Plasmid screening at high colony density. Methods Enzymol. 1983;100:333–342. doi: 10.1016/0076-6879(83)00066-x. [DOI] [PubMed] [Google Scholar]
- Kamp E. M., ter Laak E. A., de Jong M. F. Atypical Pasteurella strains producing a toxin similar to the dermonecrotic toxin of Pasteurella multocida subspecies multocida. Vet Rec. 1990 Apr 28;126(17):434–437. [PubMed] [Google Scholar]
- Kamps A. M., Kamp E. M., Smits M. A. Cloning and expression of the dermonecrotic toxin gene of Pasteurella multocida ssp. multocida in Escherichia coli. FEMS Microbiol Lett. 1990 Jan 15;55(1-2):187–190. doi: 10.1016/0378-1097(90)90192-s. [DOI] [PubMed] [Google Scholar]
- Konings R. N., Verhoeven E. J., Peeters B. P. pKUN, vectors for the separate production of both DNA strands of recombinant plasmids. Methods Enzymol. 1987;153:12–34. doi: 10.1016/0076-6879(87)53045-2. [DOI] [PubMed] [Google Scholar]
- Lax A. J., Chanter N. Cloning of the toxin gene from Pasteurella multocida and its role in atrophic rhinitis. J Gen Microbiol. 1990 Jan;136(1):81–87. doi: 10.1099/00221287-136-1-81. [DOI] [PubMed] [Google Scholar]
- Pedersen K. B., Barfod K. The aetiological significance of Bordetella bronchiseptica and Pasteurella multocida in atrophic rhinitis of swine. Nord Vet Med. 1981 Dec;33(12):513–522. [PubMed] [Google Scholar]
- Petersen S. K., Foged N. T. Cloning and expression of the Pasteurella multocida toxin gene, toxA, in Escherichia coli. Infect Immun. 1989 Dec;57(12):3907–3913. doi: 10.1128/iai.57.12.3907-3913.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rutter J. M., Luther P. D. Cell culture assay for toxigenic Pasteurella multocida from atrophic rhinitis of pigs. Vet Rec. 1984 Apr 21;114(16):393–396. doi: 10.1136/vr.114.16.393. [DOI] [PubMed] [Google Scholar]
- Saiki R. K., Scharf S., Faloona F., Mullis K. B., Horn G. T., Erlich H. A., Arnheim N. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science. 1985 Dec 20;230(4732):1350–1354. doi: 10.1126/science.2999980. [DOI] [PubMed] [Google Scholar]