A. Structure of the HSV-1 WT KOS genome from the UL53 to UL55 genes. B. Structure of the HSV-1 d106 genome from the UL53 to UL55 genes showing the CMV GFP expression cassette inserted between nts 113327–114517. C. Structure of the insert in the pPs27pd1 transfer plasmid with KOS DNA fragments from nts 112,700–113,326 and nts 114591–115,957. D. Structure of insert in transfer plasmid pd27B containing KOS DNA fragments from nts 11200–113324 and nts 114,540–115,860 flanking the CMV-multiple cloning site-poly(A) cassette.