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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Neuropsychopharmacology. 2009 Feb 4;34(7):1829–1842. doi: 10.1038/npp.2009.5

Figure 6. PKA is not involved in the induction or expression of LTPGABA.

Figure 6

(a) Single experiment showing occlusion of LTPGABA with Sp-cAMPS (10μM)-induced potentiation. Sp-cAMPS, a cAMP analog, potentiated GABAA IPSCs and occluded the potentiation induced by HFS. Inset: Averaged GABAA IPSCs recorded before (black) and after 25 minutes in Sp-cAMPS (red) and 20 minutes after HFS (green). Calibration: 10ms, 100 pA.

(b) Averaged experiments showing that Sp-cAMPS mimics LTPGABA (152 ± 10% of pre-drug values, n=5).

(c) After the IPSCs in Sp-cAMPS reached a stable potentiated level, HFS was delivered. Only the portion of the experiment after Sp-cAMPS-induced potentiation is shown. Sp-cAMPS-induced potentiation occluded further potentiation of IPSCs by HFS (100 ± 0.5% of pre-HFS values, n=6).

(d) Bath application of Rp-cAMPS (20 μM), a PKA inhibitor, had no effect on the induction or expression of LTPGABA (control LTP, filled circles: 144 ± 14% of pre-HFS values, n=10; Rp-cAMPS cells, open circles: 128 ± 10% of pre-HFS values, n=6). Rp-cAMPS was present in the bath for at least 15 minutes prior to HFS.