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. 1990 Sep;28(9):2082–2086. doi: 10.1128/jcm.28.9.2082-2086.1990

DNA probes for the identification of Nocardia asteroides.

G H Brownell 1, K E Belcher 1
PMCID: PMC268107  PMID: 2229389

Abstract

DNA probes for the rapid identification of Nocardia asteroides were obtained by constructing a genomic library of strain GUH-2 in the lambda cloning vector EMBL3. Of 50 recombinant clones tested, 2 were identified that hybridized with 31% of the N. asteroides strains in a reference collection without cross-hybridization with related members of the Actinomycetales. Additional libraries were then generated from selected strains of N. asteroides that had failed to hybridize with any of the GUH-2 clones. Four additional clones were obtained from these strains which, when pooled, provided DNA probes specific for all of the N. asteroides strains tested.

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Selected References

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