Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1990 Sep;28(9):2107–2110. doi: 10.1128/jcm.28.9.2107-2110.1990

Immunoprecipitation of human immunodeficiency virus type 2 glycoproteins by sera positive for human immunodeficiency virus type 1.

R T Espejo 1, P Uribe 1
PMCID: PMC268113  PMID: 2229392

Abstract

Analysis by radioimmunoprecipitation of serum samples from 27 different human immunodeficiency virus type 1 (HIV-1)-infected individuals residing in Chile showed that the sera of 26% of these individuals also react with glycoprotein gp125 of HIV type 2 (HIV-2). This cross-reaction seems to reflect a qualitative difference among infected individuals, because the titer of antibodies against gp120 of HIV-1 in the cross-reacting samples did not differ significantly from that in the non-cross-reacting samples. Most of the HIV-1-seropositive sera, including many that did not react with gp125 of HIV-2, reacted with gp140, the precursor of HIV-2 glycoproteins. The observed cross-reactions allowed us to distinguish three groups of HIV-1-infected individuals: (i) those whose sera react with both gp140 and gp125, (ii) those whose sera react with gp140, and (iii) those whose sera react with neither of these glycoproteins. The possible cause and significance of these differences is under study.

Full text

PDF
2107

Images in this article

2108Image
on p.2108
2108Image
on p.2108
2109Image
on p.2109
2109Image
on p.2109

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Buttò S., Verani P., Titti F., Rezza G., Sernicola L., Rossi G. B. Simultaneous seropositivity to HIV-1 and HIV-2 in Italian drug abusers. AIDS. 1988 Apr;2(2):139–140. [PubMed] [Google Scholar]
  2. Clavel F., Guétard D., Brun-Vézinet F., Chamaret S., Rey M. A., Santos-Ferreira M. O., Laurent A. G., Dauguet C., Katlama C., Rouzioux C. Isolation of a new human retrovirus from West African patients with AIDS. Science. 1986 Jul 18;233(4761):343–346. doi: 10.1126/science.2425430. [DOI] [PubMed] [Google Scholar]
  3. Clavel F., Mansinho K., Chamaret S., Guetard D., Favier V., Nina J., Santos-Ferreira M. O., Champalimaud J. L., Montagnier L. Human immunodeficiency virus type 2 infection associated with AIDS in West Africa. N Engl J Med. 1987 May 7;316(19):1180–1185. doi: 10.1056/NEJM198705073161903. [DOI] [PubMed] [Google Scholar]
  4. Cortes E., Detels R., Aboulafia D., Li X. L., Moudgil T., Alam M., Bonecker C., Gonzaga A., Oyafuso L., Tondo M. HIV-1, HIV-2, and HTLV-I infection in high-risk groups in Brazil. N Engl J Med. 1989 Apr 13;320(15):953–958. doi: 10.1056/NEJM198904133201501. [DOI] [PubMed] [Google Scholar]
  5. Foucault C., Lopez O., Jourdan G., Fournel J. J., Perret P., Gluckman J. C. Double HIV-1 and HIV-2 seropositivity among blood donors. Lancet. 1987 Jul 18;2(8551):165–166. doi: 10.1016/s0140-6736(87)92372-5. [DOI] [PubMed] [Google Scholar]
  6. HIV infection in Brazil. N Engl J Med. 1989 Sep 21;321(12):830–832. doi: 10.1056/NEJM198909213211213. [DOI] [PubMed] [Google Scholar]
  7. Jackson J. B., Balfour H. H., Jr Practical diagnostic testing for human immunodeficiency virus. Clin Microbiol Rev. 1988 Jan;1(1):124–138. doi: 10.1128/cmr.1.1.124. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  9. Poss M. L., Mullins J. I., Hoover E. A. Posttranslational modifications distinguish the envelope glycoprotein of the immunodeficiency disease-inducing feline leukemia virus retrovirus. J Virol. 1989 Jan;63(1):189–195. doi: 10.1128/jvi.63.1.189-195.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rey M. A., Krust B., Laurent A. G., Montagnier L., Hovanessian A. G. Characterization of human immunodeficiency virus type 2 envelope glycoproteins: dimerization of the glycoprotein precursor during processing. J Virol. 1989 Feb;63(2):647–658. doi: 10.1128/jvi.63.2.647-658.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Schulz T. F., Oberhuber W., Hofbauer J. M., Hengster P., Larcher C., Gürtler L. C., Tedder R., Wachter H., Dierich M. P. Recombinant peptides derived from the env-gene of HIV-2 in the serodiagnosis of HIV-2 infections. AIDS. 1989 Mar;3(3):165–172. doi: 10.1097/00002030-198903000-00007. [DOI] [PubMed] [Google Scholar]
  12. Tedder R. S., O'Connor T., Hughes A., N'jie H., Corrah T., Whittle H. Envelope cross-reactivity in Western blot for HIV-1 and HIV-2 may not indicate dual infection. Lancet. 1988 Oct 22;2(8617):927–930. doi: 10.1016/s0140-6736(88)92598-6. [DOI] [PubMed] [Google Scholar]
  13. Veronese F. D., DeVico A. L., Copeland T. D., Oroszlan S., Gallo R. C., Sarngadharan M. G. Characterization of gp41 as the transmembrane protein coded by the HTLV-III/LAV envelope gene. Science. 1985 Sep 27;229(4720):1402–1405. doi: 10.1126/science.2994223. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES