Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1990 Sep;28(9):2111–2113. doi: 10.1128/jcm.28.9.2111-2113.1990

Characterization of a new antigenic type, Kuroki, of Rickettsia tsutsugamushi isolated from a patient in Japan.

N Ohashi 1, A Tamura 1, H Sakurai 1, S Yamamoto 1
PMCID: PMC268114  PMID: 2121793

Abstract

The Kuroki strain of Rickettsia tsutsugamushi, isolated from a patient in Kyushu, Japan, has a major, type-specific antigenic polypeptide which is distinct from the prototype strains in size (58 kilodaltons), in antigenicity, and in its cleavage pattern with N-chlorosuccinimide. These results indicate that Kuroki is another antigenic type of R. tsutsugamushi.

Full text

PDF
2111

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amano K., Tamura A., Ohashi N., Urakami H., Kaya S., Fukushi K. Deficiency of peptidoglycan and lipopolysaccharide components in Rickettsia tsutsugamushi. Infect Immun. 1987 Sep;55(9):2290–2292. doi: 10.1128/iai.55.9.2290-2292.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Elisberg B. L., Campbell J. M., Bozeman F. M. Antigenic diversity of rickettsia tsutsugamushi: epidemiologic and ecologic significance. J Hyg Epidemiol Microbiol Immunol. 1968;12(1):18–25. [PubMed] [Google Scholar]
  3. Lischwe M. A., Ochs D. A new method for partial peptide mapping using N-chlorosuccinimide/urea and peptide silver staining in sodium dodecyl sulfate-polyacrylamide gels. Anal Biochem. 1982 Dec;127(2):453–457. doi: 10.1016/0003-2697(82)90203-2. [DOI] [PubMed] [Google Scholar]
  4. Merril C. R., Goldman D., Sedman S. A., Ebert M. H. Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins. Science. 1981 Mar 27;211(4489):1437–1438. doi: 10.1126/science.6162199. [DOI] [PubMed] [Google Scholar]
  5. Murata M., Yoshida Y., Osono M., Ohashi N., Oyanagi M., Urakami H., Tamura A., Nogami S., Tanaka H., Kawamura A., Jr Production and characterization of monoclonal strain-specific antibodies against prototype strains of Rickettsia tsutsugamushi. Microbiol Immunol. 1986;30(7):599–610. doi: 10.1111/j.1348-0421.1986.tb02987.x. [DOI] [PubMed] [Google Scholar]
  6. Ohashi N., Tamura A., Ohta M., Hayashi K. Purification and partial characterization of a type-specific antigen of Rickettsia tsutsugamushi. Infect Immun. 1989 May;57(5):1427–1431. doi: 10.1128/iai.57.5.1427-1431.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ohashi N., Tamura A., Suto T. Immunoblotting analysis of anti-rickettsial antibodies produced in patients of Tsutsugamushi disease. Microbiol Immunol. 1988;32(11):1085–1092. doi: 10.1111/j.1348-0421.1988.tb01473.x. [DOI] [PubMed] [Google Scholar]
  8. Tamura A., Ohashi N., Urakami H., Takahashi K., Oyanagi M. Analysis of polypeptide composition and antigenic components of Rickettsia tsutsugamushi by polyacrylamide gel electrophoresis and immunoblotting. Infect Immun. 1985 Jun;48(3):671–675. doi: 10.1128/iai.48.3.671-675.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Tamura A., Takahashi K., Tsuruhara T., Urakami H., Miyamura S., Sekikawa H., Kenmotsu M., Shibata M., Abe S., Nezu H. Isolation of Rickettsia tsutsugamushi antigenically different from Kato, Karp, and Gilliam strains from patients. Microbiol Immunol. 1984;28(8):873–882. doi: 10.1111/j.1348-0421.1984.tb00743.x. [DOI] [PubMed] [Google Scholar]
  10. Yamamoto S., Kawabata N., Ooura K., Murata M., Minamishima Y. [Antigenic types of Rickettsia tsutsugamushi isolated from patients with tsutsugamushi fever and their distribution in Miyazaki Prefecture]. Kansenshogaku Zasshi. 1989 Feb;63(2):109–117. doi: 10.11150/kansenshogakuzasshi1970.63.109. [DOI] [PubMed] [Google Scholar]
  11. Yamamoto S., Kawabata N., Tamura A., Urakami H., Ohashi N., Murata M., Yoshida Y., Kawamura A., Jr Immunological properties of Rickettsia tsutsugamushi, Kawasaki strain, isolated from a patient in Kyushu. Microbiol Immunol. 1986;30(7):611–620. doi: 10.1111/j.1348-0421.1986.tb02988.x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES