Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1990 Sep;28(9):2114–2116. doi: 10.1128/jcm.28.9.2114-2116.1990

Bacillus species pseudobacteremia traced to contaminated gloves used in collection of blood from patients with acquired immunodeficiency syndrome.

M K York 1
PMCID: PMC268115  PMID: 2229393

Abstract

Ten nonpathogenic Bacillus isolates were obtained from blood cultures collected over a 2-year period. Eight of these isolates were from patient with acquired immunodeficiency syndrome, and seven were recovered from blood cultures obtained in outpatient clinics. Five cases occurred during a 5-month period. These five cases were clinically evaluated, and the Bacillus isolates were characterized. The same Bacillus species was isolated from nonsterile gloves from the same lot worn by phlebotomists for blood collection in the outpatient clinics during this period, implicating the gloves as the cause of this pseudoepidemic. Awareness of the nonsterile nature of gloves used by laboratory personnel should be considered in the evaluation of Bacillus spp. in blood cultures.

Full text

PDF
2114

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOYETT D. P., RIGHTS F. L. Heretofore undescribed aerobic spore forming bacillus in child with meningitis. J Am Med Assoc. 1952 Apr 5;148(14):1223–1224. [PubMed] [Google Scholar]
  2. Berger S. A. Pseudobacteremia due to contaminated alcohol swabs. J Clin Microbiol. 1983 Oct;18(4):974–975. doi: 10.1128/jcm.18.4.974-975.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bradley S. F., Wilson K. H., Rosloniec M. A., Kauffman C. A. Recurrent pseudobacteremias traced to a radiometric blood culture device. Infect Control. 1987 Jul;8(7):281–283. doi: 10.1017/s0195941700066248. [DOI] [PubMed] [Google Scholar]
  4. Cotton D. J., Gill V. J., Marshall D. J., Gress J., Thaler M., Pizzo P. A. Clinical features and therapeutic interventions in 17 cases of Bacillus bacteremia in an immunosuppressed patient population. J Clin Microbiol. 1987 Apr;25(4):672–674. doi: 10.1128/jcm.25.4.672-674.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Craven D. E., Lichtenberg D. A., Browne K. F., Coffey D. M., Treadwell T. L., McCabe W. R. Pseudobacteremia traced to cross-contamination by an automated blood culture analyzer. Infect Control. 1984 Feb;5(2):75–78. doi: 10.1017/s0195941700058987. [DOI] [PubMed] [Google Scholar]
  6. FARRAR W. E., Jr Serious infections due to "non-pathogenic" organisms of the genus Bacillus. Review of their status as pathogens. Am J Med. 1963 Jan;34:134–141. doi: 10.1016/0002-9343(63)90047-0. [DOI] [PubMed] [Google Scholar]
  7. Guiot H. F., de Planque M. M., Richel D. J., van't Wout J. W. Bacillus cereus: a snake in the grass for granulocytopenic patients. J Infect Dis. 1986 Jun;153(6):1186–1186. doi: 10.1093/infdis/153.6.1186. [DOI] [PubMed] [Google Scholar]
  8. Gurevich I., Tafuro P., Krystofiak S. P., Kalter R. D., Cunha B. A. Three clusters of Bacillus pseudobacteremia related to a radiometric blood culture analyzer. Infect Control. 1984 Feb;5(2):71–74. doi: 10.1017/s0195941700058975. [DOI] [PubMed] [Google Scholar]
  9. Hoffman P. C., Arnow P. M., Goldmann D. A., Parrott P. L., Stamm W. E., McGowan J. E., Jr False-positive blood cultures. Association with nonsterile blood collection tubes. JAMA. 1976 Nov 1;236(18):2073–2075. [PubMed] [Google Scholar]
  10. Ihde D. C., Armstrong D. Clinical spectrum of infection due to Bacillus species. Am J Med. 1973 Dec;55(6):839–845. doi: 10.1016/0002-9343(73)90266-0. [DOI] [PubMed] [Google Scholar]
  11. John J. F., Jr, Bannister E. R. Pseudobacteremia. Infect Control. 1984 Feb;5(2):69–70. doi: 10.1017/s0195941700058963. [DOI] [PubMed] [Google Scholar]
  12. Maki D. G. Through a glass darkly. Nosocomial pseudoepidemics and pseudobacteremias. Arch Intern Med. 1980 Jan;140(1):26–28. [PubMed] [Google Scholar]
  13. McNeil M. M., Davis B. J., Solomon S. L., Anderson R. L., Shulman S. T., Gardner S., Kabat K., Martone W. J. Ewingella americana: recurrent pseudobacteremia from a persistent environmental reservoir. J Clin Microbiol. 1987 Mar;25(3):498–500. doi: 10.1128/jcm.25.3.498-500.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Noble R. C., Reeves S. A. Bacillus species pseudosepsis caused by contaminated commercial blood culture media. JAMA. 1974 Nov 18;230(7):1002–1004. [PubMed] [Google Scholar]
  15. Tuazon C. U., Murray H. W., Levy C., Solny M. N., Curtin J. A., Sheagren J. N. Serious infections from Bacillus sp. JAMA. 1979 Mar 16;241(11):1137–1140. [PubMed] [Google Scholar]
  16. Weinstein R. A., Stamm W. E. Pseudoepidemics in hospital. Lancet. 1977 Oct 22;2(8043):862–864. doi: 10.1016/s0140-6736(77)90793-0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES