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. 1990 Oct;28(10):2187–2190. doi: 10.1128/jcm.28.10.2187-2190.1990

Detection of Epstein-Barr virus by polymerase chain reaction.

A Telenti 1, W F Marshall 1, T F Smith 1
PMCID: PMC268144  PMID: 2172284

Abstract

The polymerase chain reaction (PCR) was used to study DNA extracted from the blood of 25 transplant patients, 5 patients with infectious mononucleosis, and 13 healthy subjects and autopsy or biopsy tissue from 29 patients with lymphoproliferative disorders. Primers were directed to conserved regions of the Epstein-Barr virus (EBV) genome encoding capsid protein gp220 and Epstein-Barr nuclear antigen 1. Specific EBV amplification was found in the blood of 11 of 25 transplant patients and all patients with infectious mononucleosis. All patients with lymphoproliferative disorders occurring in the presence of immunosuppression (eight organ transplant patients and two patients with acquired immunodeficiency syndrome) had biopsies positive for EBV by PCR. Only 1 of 19 samples from lymphomas or leukemias unrelated to immunosuppression contained EBV. PCR confirmed the very close association of EBV and lymphoproliferative disorders occurring in the presence of immunosuppression. The significance of detecting EBV sequences in the blood of transplant patients, particularly in relationship to lymphoproliferation, requires further study.

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Selected References

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  1. Bignon Y. J., Bernard D., Curé H., Fonck Y., Pauchard J., Travade P., Legros M., Dastugue B., Plagne R. Detection of Epstein-Barr viral genomes in lymph nodes of Hodgkin's disease patients. Mol Carcinog. 1990;3(1):9–11. doi: 10.1002/mc.2940030105. [DOI] [PubMed] [Google Scholar]
  2. Birx D. L., Redfield R. R., Tosato G. Defective regulation of Epstein-Barr virus infection in patients with acquired immunodeficiency syndrome (AIDS) or AIDS-related disorders. N Engl J Med. 1986 Apr 3;314(14):874–879. doi: 10.1056/NEJM198604033141403. [DOI] [PubMed] [Google Scholar]
  3. De Waele M., Thielemans C., Van Camp B. K. Characterization of immunoregulatory T cells in EBV-induced infectious mononucleosis by monoclonal antibodies. N Engl J Med. 1981 Feb 19;304(8):460–462. doi: 10.1056/NEJM198102193040804. [DOI] [PubMed] [Google Scholar]
  4. Fermand J. P., Gozlan J., Bendelac A., Delauche-Cavallier M. C., Brouet J. C., Morinet F. Detection of Epstein-Barr virus in epidermal skin lesions of an immunocompromised patient. Ann Intern Med. 1990 Apr 1;112(7):511–515. doi: 10.7326/0003-4819-112-7-511. [DOI] [PubMed] [Google Scholar]
  5. Fleisher G. R., Collins M., Fager S. Limitations of available tests for diagnosis of infectious mononucleosis. J Clin Microbiol. 1983 Apr;17(4):619–624. doi: 10.1128/jcm.17.4.619-624.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gustafson S., Proper J. A., Bowie E. J., Sommer S. S. Parameters affecting the yield of DNA from human blood. Anal Biochem. 1987 Sep;165(2):294–299. doi: 10.1016/0003-2697(87)90272-7. [DOI] [PubMed] [Google Scholar]
  7. Ho M., Miller G., Atchison R. W., Breinig M. K., Dummer J. S., Andiman W., Starzl T. E., Eastman R., Griffith B. P., Hardesty R. L. Epstein-Barr virus infections and DNA hybridization studies in posttransplantation lymphoma and lymphoproliferative lesions: the role of primary infection. J Infect Dis. 1985 Nov;152(5):876–886. doi: 10.1093/infdis/152.5.876. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Katz B. Z., Raab-Traub N., Miller G. Latent and replicating forms of Epstein-Barr virus DNA in lymphomas and lymphoproliferative diseases. J Infect Dis. 1989 Oct;160(4):589–598. doi: 10.1093/infdis/160.4.589. [DOI] [PubMed] [Google Scholar]
  9. Lipscomb H., Tatsumi E., Harada S., Yetz J., Davis J., Bechtold T., Volsky D. J., Kuszynski C., Purtilo D. T., Sonnabend J. Epstein-Barr virus and chronic lymphadenomegaly in male homosexuals with acquired immunodeficiency syndrome (AIDS). AIDS Res. 1983 1984;1(1):59–82. doi: 10.1089/aid.1.1983.1.59. [DOI] [PubMed] [Google Scholar]
  10. Martin P. J., Shulman H. M., Schubach W. H., Hansen J. A., Fefer A., Miller G., Thomas E. D. Fatal Epstein-Barr-virus-associated proliferation of donor B cells after treatment of acute graft-versus-host disease with a murine anti-T-cell antibody. Ann Intern Med. 1984 Sep;101(3):310–315. doi: 10.7326/0003-4819-101-3-310. [DOI] [PubMed] [Google Scholar]
  11. Nalesnik M. A., Jaffe R., Starzl T. E., Demetris A. J., Porter K., Burnham J. A., Makowka L., Ho M., Locker J. The pathology of posttransplant lymphoproliferative disorders occurring in the setting of cyclosporine A-prednisone immunosuppression. Am J Pathol. 1988 Oct;133(1):173–192. [PMC free article] [PubMed] [Google Scholar]
  12. Okano M., Thiele G. M., Davis J. R., Grierson H. L., Purtilo D. T. Epstein-Barr virus and human diseases: recent advances in diagnosis. Clin Microbiol Rev. 1988 Jul;1(3):300–312. doi: 10.1128/cmr.1.3.300. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Petersen J. M., Tubbs R. R., Savage R. A., Calabrese L. C., Proffitt M. R., Manolova Y., Manolov G., Shumaker A., Tatsumi E., McClain K. Small noncleaved B cell Burkitt-like lymphoma with chromosome t(8;14) translocation and Epstein-Barr virus nuclear-associated antigen in a homosexual man with acquired immune deficiency syndrome. Am J Med. 1985 Jan;78(1):141–148. doi: 10.1016/0002-9343(85)90475-9. [DOI] [PubMed] [Google Scholar]
  14. Rocchi G., Felici A., Ragona G., Heinz A. Quantitative evaluation of Epstein-Barr-virus-infected mononuclear peripheral blood leukocytes in infectious mononucleosis. N Engl J Med. 1977 Jan 20;296(3):132–134. doi: 10.1056/NEJM197701202960302. [DOI] [PubMed] [Google Scholar]
  15. Rogers B. B., Alpert L. C., Hine E. A., Buffone G. J. Analysis of DNA in fresh and fixed tissue by the polymerase chain reaction. Am J Pathol. 1990 Mar;136(3):541–548. [PMC free article] [PubMed] [Google Scholar]
  16. Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988 Jan 29;239(4839):487–491. doi: 10.1126/science.2448875. [DOI] [PubMed] [Google Scholar]
  17. Saito I., Servenius B., Compton T., Fox R. I. Detection of Epstein-Barr virus DNA by polymerase chain reaction in blood and tissue biopsies from patients with Sjogren's syndrome. J Exp Med. 1989 Jun 1;169(6):2191–2198. doi: 10.1084/jem.169.6.2191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Schochetman G., Ou C. Y., Jones W. K. Polymerase chain reaction. J Infect Dis. 1988 Dec;158(6):1154–1157. doi: 10.1093/infdis/158.6.1154. [DOI] [PubMed] [Google Scholar]
  19. Shibata D. K., Arnheim N., Martin W. J. Detection of human papilloma virus in paraffin-embedded tissue using the polymerase chain reaction. J Exp Med. 1988 Jan 1;167(1):225–230. doi: 10.1084/jem.167.1.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wilkinson A. H., Smith J. L., Hunsicker L. G., Tobacman J., Kapelanski D. P., Johnson M., Wright F. H., Behrendt D. M., Corry R. J. Increased frequency of posttransplant lymphomas in patients treated with cyclosporine, azathioprine, and prednisone. Transplantation. 1989 Feb;47(2):293–296. doi: 10.1097/00007890-198902000-00020. [DOI] [PubMed] [Google Scholar]

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