Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1990 Oct;28(10):2248–2252. doi: 10.1128/jcm.28.10.2248-2252.1990

Application of monoclonal antibodies to the detection of black-pigmented Bacteroides spp. in subgingival plaques by immunoslot blot assay.

S Hanazawa 1, S Tanaka 1, M Kin 1, S Amano 1, K Nakada 1, T Masuda 1, S Kitano 1
PMCID: PMC268156  PMID: 2229348

Abstract

The aim of the present study was to assess the application of monoclonal antibodies to the detection of black-pigmented Bacteroides spp. in subgingival plaques by immunoslot blot assay. Subgingival plaque samples from adult periodontal patients were examined by immunoslot blot assay with monoclonal antibodies that specifically recognize Bacteroides gingivalis, Bacteroides intermedius serogroups I and II, and Bacteroides melaninogenicus. The assay can detect specifically these Bacteroides spp. in the subgingival plaques. Therefore, we investigated the distribution of these Bacteroides spp. in the subgingival plaques of patients classified by Russell's periodontal index. Reactivities of their plaques with monoclonal antibodies toward B. gingivalis and B. intermedius serogroup I were clearly related to the severity of the periodontal disease, but this was not the case with B. intermedius serogroup II and B. melaninogenicus. These results indicate that this immunoslot blot assay using monoclonal antibodies toward these Bacteroides spp. provides simple detection and monitoring of these organisms in periodontal patients.

Full text

PDF
2248

Images in this article

2250Image
on p.2250
2250Image
on p.2250

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  2. Ebersole J. L., Taubman M. A., Smith D. J., Genco R. J., Frey D. E. Human immune responses to oral micro-organisms. I. Association of localized juvenile periodontitis (LJP) with serum antibody responses to Actinobacillus actinomycetemcomitans. Clin Exp Immunol. 1982 Jan;47(1):43–52. [PMC free article] [PubMed] [Google Scholar]
  3. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
  4. French C. K., Savitt E. D., Simon S. L., Eklund S. M., Chen M. C., Klotz L. C., Vaccaro K. K. DNA probe detection of periodontal pathogens. Oral Microbiol Immunol. 1986 Nov;1(1):58–62. doi: 10.1111/j.1399-302x.1986.tb00320.x. [DOI] [PubMed] [Google Scholar]
  5. Gmür R. Applicability of monoclonal antibodies to quantitatively monitor subgingival plaque for specific bacteria. Oral Microbiol Immunol. 1988 Dec;3(4):187–191. doi: 10.1111/j.1399-302x.1988.tb00008.x. [DOI] [PubMed] [Google Scholar]
  6. Gmür R., Strub J. R., Guggenheim B. Prevalence of Bacteroides forsythus and Bacteroides gingivalis in subgingival plaque of prosthodontically treated patients on short recall. J Periodontal Res. 1989 Mar;24(2):113–120. doi: 10.1111/j.1600-0765.1989.tb00865.x. [DOI] [PubMed] [Google Scholar]
  7. Hanazawa S., Saitoh K., Ohmori Y., Nishihara H., Fujiwara S., Kitano S. Production of monoclonal antibodies that recognize specific and cross-reactive antigens of Bacteroides gingivalis. Infect Immun. 1984 Oct;46(1):285–287. doi: 10.1128/iai.46.1.285-287.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Listgarten M. A., Lai C. H., Evian C. I. Comparative antibody titers to Actinobacillus actinomycetemcomitans in juvenile periodontitis, chronic periodontitis and periodontally healthy subjects. J Clin Periodontol. 1981 Jun;8(3):155–164. doi: 10.1111/j.1600-051x.1981.tb02027.x. [DOI] [PubMed] [Google Scholar]
  9. Mashimo P. A., Ellison S. A. Diffusate media for cultivation of oral anaerobic bacteria. Koku Eisei Gakkai Zasshi. 1972 Mar;22(1):38–44. doi: 10.5834/jdh.22.38. [DOI] [PubMed] [Google Scholar]
  10. Miyoshi T., Hanazawa S., Hirose K., Saitoh K., Amano S., Ohmori Y., Kitano S. Humoral antibody response against Bacteroides gingivalis-specific antigen recognized by monoclonal antibody in adult periodontal patients. Infect Immun. 1986 Aug;53(2):366–371. doi: 10.1128/iai.53.2.366-371.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Moncla B. J., Strockbine L., Braham P., Karlinsey J., Roberts M. C. The use of whole-cell DNA probes for the identification of Bacteroides intermedius isolates in a dot blot assay. J Dent Res. 1988 Oct;67(10):1267–1270. doi: 10.1177/00220345880670100401. [DOI] [PubMed] [Google Scholar]
  12. Mouton C., Hammond P. G., Slots J., Genco R. J. Serum antibodies to oral Bacteroides asaccharolyticus (Bacteroides gingivalis): relationship to age and periondontal disease. Infect Immun. 1981 Jan;31(1):182–192. doi: 10.1128/iai.31.1.182-192.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ohmori Y., Honda K., Kikuchu H., Hanazawa S., Amano S., Hirose K., Takeshita A., Katoh T., Kitano S. Inducing effect of periodontopathic bacteria on interleukin-1 production by mouse peritoneal macrophages. Oral Microbiol Immunol. 1988 Dec;3(4):169–172. doi: 10.1111/j.1399-302x.1988.tb00004.x. [DOI] [PubMed] [Google Scholar]
  14. RUSSELL A. L. A system of classification and scoring for prevalence surveys of periodontal disease. J Dent Res. 1956 Jun;35(3):350–359. doi: 10.1177/00220345560350030401. [DOI] [PubMed] [Google Scholar]
  15. Savitt E. D., Strzempko M. N., Vaccaro K. K., Peros W. J., French C. K. Comparison of cultural methods and DNA probe analyses for the detection of Actinobacillus actinomycetemcomitans, Bacteroides gingivalis, and Bacteroides intermedius in subgingival plaque samples. J Periodontol. 1988 Jul;59(7):431–438. doi: 10.1902/jop.1988.59.7.431. [DOI] [PubMed] [Google Scholar]
  16. Slots J., Hafström C., Rosling B., Dahlén G. Detection of Actinobacillus actinomycetemcomitans and Bacteroides gingivalis in subgingival smears by the indirect fluorescent-antibody technique. J Periodontal Res. 1985 Nov;20(6):613–620. doi: 10.1111/j.1600-0765.1985.tb00845.x. [DOI] [PubMed] [Google Scholar]
  17. Slots J., Reynolds H. S., Genco R. J. Actinobacillus actinomycetemcomitans in human periodontal disease: a cross-sectional microbiological investigation. Infect Immun. 1980 Sep;29(3):1013–1020. doi: 10.1128/iai.29.3.1013-1020.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Slots J. Subgingival microflora and periodontal disease. J Clin Periodontol. 1979 Oct;6(5):351–382. doi: 10.1111/j.1600-051x.1979.tb01935.x. [DOI] [PubMed] [Google Scholar]
  19. Strzempko M. N., Simon S. L., French C. K., Lippke J. A., Raia F. F., Savitt E. D., Vaccaro K. K. A cross-reactivity study of whole genomic DNA probes for Haemophilus actinomycetemcomitans, Bacteroides intermedius, and Bacteroides gingivalis. J Dent Res. 1987 Oct;66(10):1543–1546. doi: 10.1177/00220345870660100601. [DOI] [PubMed] [Google Scholar]
  20. White D., Mayrand D. Association of oral Bacteroides with gingivitis and adult periodontitis. J Periodontal Res. 1981 May;16(3):259–265. doi: 10.1111/j.1600-0765.1981.tb00974.x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES