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. 1990 Oct;28(10):2351–2353. doi: 10.1128/jcm.28.10.2351-2353.1990

Differentiation of Genes Coding for Escherichia coli Verotoxin 2 and the Verotoxin Associated with Porcine Edema Disease (VTe) by the Polymerase Chain Reaction

W M Johnson 1,*, D R Pollard 2, H Lior 1, S D Tyler 2, K R Rozee 2
PMCID: PMC268176  PMID: 2229362

Abstract

Two sets of synthetic oligonucleotide primers were used in a polymerase chain reaction adaptation to distinguish the closely related genes for type 2 verotoxin (VT2 or Shiga-like toxin [SLT-II]) and the verotoxin associated with porcine edema disease (VTe or SLT-II variant [SLT-IIv]) in Escherichia coli.

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Selected References

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  1. Brown J. E., Sethabutr O., Jackson M. P., Lolekha S., Echeverria P. Hybridization of Escherichia coli producing Shiga-like toxin I, Shiga-like toxin II, and a variant of Shiga-like toxin II with synthetic oligonucleotide probes. Infect Immun. 1989 Sep;57(9):2811–2814. doi: 10.1128/iai.57.9.2811-2814.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dobrescu L. New biological effect of edema disease principle (Escherichia coli-neurotoxin) and its use as an in vitro for this toxin. Am J Vet Res. 1983 Jan;44(1):31–34. [PubMed] [Google Scholar]
  3. Gyles C. L., De Grandis S. A., MacKenzie C., Brunton J. L. Cloning and nucleotide sequence analysis of the genes determining verocytotoxin production in a porcine edema disease isolate of Escherichia coli. Microb Pathog. 1988 Dec;5(6):419–426. doi: 10.1016/0882-4010(88)90003-4. [DOI] [PubMed] [Google Scholar]
  4. Johnson W. M., Lior H. A new heat-labile cytolethal distending toxin (CLDT) produced by Escherichia coli isolates from clinical material. Microb Pathog. 1988 Feb;4(2):103–113. doi: 10.1016/0882-4010(88)90052-6. [DOI] [PubMed] [Google Scholar]
  5. Karch H., Meyer T. Evaluation of oligonucleotide probes for identification of shiga-like-toxin-producing Escherichia coli. J Clin Microbiol. 1989 Jun;27(6):1180–1186. doi: 10.1128/jcm.27.6.1180-1186.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Karmali M. A. Infection by verocytotoxin-producing Escherichia coli. Clin Microbiol Rev. 1989 Jan;2(1):15–38. doi: 10.1128/cmr.2.1.15. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Linggood M. A., Thompson J. M. Verotoxin production among porcine strains of Escherichia coli and its association with oedema disease. J Med Microbiol. 1987 Dec;24(4):359–362. doi: 10.1099/00222615-24-4-359. [DOI] [PubMed] [Google Scholar]
  8. Mainil J., Daube G., Deprez P., Kaeckenbeeck A., Pohl P. Detection and identification of pathotypes of verocytotoxigenic Escherichia coli isolated from weaned piglets using gene probes for seven E. coli toxins. FEMS Microbiol Lett. 1989 Jun;50(3):345–349. doi: 10.1016/0378-1097(89)90443-6. [DOI] [PubMed] [Google Scholar]
  9. Meyer T., Bitzan M., Sandkamp O., Karch H. Synthetic oligodeoxyribonucleotide probes to detect verocytotoxin-producing Escherichia coli in diseased pigs. FEMS Microbiol Lett. 1989 Jan 15;48(2):247–252. doi: 10.1111/j.1574-6968.1989.tb03308.x. [DOI] [PubMed] [Google Scholar]
  10. Newland J. W., Neill R. J. DNA probes for Shiga-like toxins I and II and for toxin-converting bacteriophages. J Clin Microbiol. 1988 Jul;26(7):1292–1297. doi: 10.1128/jcm.26.7.1292-1297.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Oku Y., Yutsudo T., Hirayama T., O'Brien A. D., Takeda Y. Purification and some properties of a Vero toxin from a human strain of Escherichia coli that is immunologically related to Shiga-like toxin II (VT2). Microb Pathog. 1989 Feb;6(2):113–122. doi: 10.1016/0882-4010(89)90014-4. [DOI] [PubMed] [Google Scholar]
  12. Pollard D. R., Johnson W. M., Lior H., Tyler S. D., Rozee K. R. Rapid and specific detection of verotoxin genes in Escherichia coli by the polymerase chain reaction. J Clin Microbiol. 1990 Mar;28(3):540–545. doi: 10.1128/jcm.28.3.540-545.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Weinstein D. L., Jackson M. P., Samuel J. E., Holmes R. K., O'Brien A. D. Cloning and sequencing of a Shiga-like toxin type II variant from Escherichia coli strain responsible for edema disease of swine. J Bacteriol. 1988 Sep;170(9):4223–4230. doi: 10.1128/jb.170.9.4223-4230.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]

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