Abstract
Q fever, caused by Coxiella burnetii, may be acute or chronic. Only a few strains of C. burnetii have been isolated due to the difficulty and hazard of isolation. We report here the isolation using a centrifugation shell vial technique of 16 new strains from patients suffering chronic Q fever. Twenty-four samples were inoculated onto human embryonic lung (HEL) fibroblast cell monolayers growing in shell vials. C. burnetii was detected 6 days later by using immunofluorescence. Samples from valves (n = 10), arterial prostheses (n = 2), bone (n = 3), skin biopsy (n = 1), bone marrow (n = 1), and blood (n = 5) from 16 patients were successfully cultured. Two cerebrospinal fluid samples from two patients were negative. The strains were subcultured in HEL cells and are now established. The technique is sensitive and less hazardous than animal inoculation. We recommend the shell vial technique for isolation of C. burnetii.
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- Ellis M. E., Smith C. C., Moffat M. A. Chronic or fatal Q-fever infection: a review of 16 patients seen in North-East Scotland (1967-80). Q J Med. 1983 Winter;52(205):54–66. [PubMed] [Google Scholar]
- Espejo-Arenas E., Raoult D. First isolates of Rickettsia conorii in Spain using a centrifugation-shell vial assay. J Infect Dis. 1989 Jun;159(6):1158–1159. doi: 10.1093/infdis/159.6.1158. [DOI] [PubMed] [Google Scholar]
- Hackstadt T., Peacock M. G., Hitchcock P. J., Cole R. L. Lipopolysaccharide variation in Coxiella burnetti: intrastrain heterogeneity in structure and antigenicity. Infect Immun. 1985 May;48(2):359–365. doi: 10.1128/iai.48.2.359-365.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Haldane E. V., Marrie T. J., Faulkner R. S., Lee S. H., Cooper J. H., MacPherson D. D., Montague T. J. Endocarditis due to Q fever in Nova Scotia: experience with five patients in 1981-1982. J Infect Dis. 1983 Dec;148(6):978–985. doi: 10.1093/infdis/148.6.978. [DOI] [PubMed] [Google Scholar]
- Marrero M., Raoult D. Centrifugation-shell vial technique for rapid detection of Mediterranean spotted fever rickettsia in blood culture. Am J Trop Med Hyg. 1989 Feb;40(2):197–199. doi: 10.4269/ajtmh.1989.40.197. [DOI] [PubMed] [Google Scholar]
- Raoult D., Bollini G., Gallais H. Osteoarticular infection due to Coxiella burnetii. J Infect Dis. 1989 Jun;159(6):1159–1160. doi: 10.1093/infdis/159.6.1159. [DOI] [PubMed] [Google Scholar]
- Raoult D., Etienne J., Massip P., Iaocono S., Prince M. A., Beaurain P., Benichou S., Auvergnat J. C., Mathieu P., Bachet P. Q fever endocarditis in the south of France. J Infect Dis. 1987 Mar;155(3):570–573. doi: 10.1093/infdis/155.3.570. [DOI] [PubMed] [Google Scholar]
- Raoult D., Piquet P., Gallais H., de Micco C., Drancourt M., Casanova P. Coxiella burnetii infection of a vascular prosthesis. N Engl J Med. 1986 Nov 20;315(21):1358–1359. [PubMed] [Google Scholar]
- Samuel J. E., Frazier M. E., Mallavia L. P. Correlation of plasmid type and disease caused by Coxiella burnetii. Infect Immun. 1985 Sep;49(3):775–779. doi: 10.1128/iai.49.3.775-779.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]