Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1990 Nov;28(11):2496–2499. doi: 10.1128/jcm.28.11.2496-2499.1990

Rapid screening for B19 parvovirus DNA in clinical specimens with a digoxigenin-labeled DNA hybridization probe.

M Zerbini 1, M Musiani 1, S Venturoli 1, G Gallinella 1, D Gibellini 1, G Gentilomi 1, M La Placa 1
PMCID: PMC268213  PMID: 2174900

Abstract

A rapid dot blot hybridization assay for the detection of B19 parvovirus DNA in human sera was developed. Small portions of four serum samples were mixed, filtered onto a nylon membrane, and hybridized with a digoxigenin-labeled DNA probe; for each membrane, 380 serum samples could be tested. When a dot was positive by the hybridization assay, the four serum samples dotted together were separately tested to identify the sample positive for B19 DNA. A total of 10,150 serum samples submitted for viral serological and laboratory investigation with no specific requests for B19 testing were analyzed. Nine serum samples were positive for B19 DNA by dot blot hybridization assay, and the results were confirmed by electron microscopy. This method has proven to be reliable, economical in terms of time and costs, and useful for large-scale screening of clinical specimens, both for diagnostic work and for a source of antigen.

Full text

PDF
2496

Images in this article

2497Image
on p.2497
2498Image
on p.2498

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anand A., Gray E. S., Brown T., Clewley J. P., Cohen B. J. Human parvovirus infection in pregnancy and hydrops fetalis. N Engl J Med. 1987 Jan 22;316(4):183–186. doi: 10.1056/NEJM198701223160403. [DOI] [PubMed] [Google Scholar]
  2. Anderson M. J., Davis L. R., Jones S. E., Pattison J. R., Serjeant G. R. The development and use of an antibody capture radioimmunoassay for specific IgM to a human parvovirus-like agent. J Hyg (Lond) 1982 Apr;88(2):309–324. doi: 10.1017/s0022172400070169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Anderson M. J., Jones S. E., Minson A. C. Diagnosis of human parvovirus infection by dot-blot hybridization using cloned viral DNA. J Med Virol. 1985 Feb;15(2):163–172. doi: 10.1002/jmv.1890150209. [DOI] [PubMed] [Google Scholar]
  4. Anderson M. J., Khousam M. N., Maxwell D. J., Gould S. J., Happerfield L. C., Smith W. J. Human parvovirus B19 and hydrops fetalis. Lancet. 1988 Mar 5;1(8584):535–535. doi: 10.1016/s0140-6736(88)91331-1. [DOI] [PubMed] [Google Scholar]
  5. Anderson M. J., Pattison J. R. The human parvovirus. Brief review. Arch Virol. 1984;82(3-4):137–148. doi: 10.1007/BF01311158. [DOI] [PubMed] [Google Scholar]
  6. Azzi A., Zakrzewska K., Gentilomi G., Musiani M., Zerbini M. Detection of B19 parvovirus infections by a dot-blot hybridization assay using a digoxigenin-labelled probe. J Virol Methods. 1990 Feb;27(2):125–133. doi: 10.1016/0166-0934(90)90129-4. [DOI] [PubMed] [Google Scholar]
  7. Brown T., Anand A., Ritchie L. D., Clewley J. P., Reid T. M. Intrauterine parvovirus infection associated with hydrops fetalis. Lancet. 1984 Nov 3;2(8410):1033–1034. doi: 10.1016/s0140-6736(84)91126-7. [DOI] [PubMed] [Google Scholar]
  8. Cohen B. J., Mortimer P. P., Pereira M. S. Diagnostic assays with monoclonal antibodies for the human serum parvovirus-like virus (SPLV). J Hyg (Lond) 1983 Aug;91(1):113–130. doi: 10.1017/s0022172400060095. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cossart Y. E., Field A. M., Cant B., Widdows D. Parvovirus-like particles in human sera. Lancet. 1975 Jan 11;1(7898):72–73. doi: 10.1016/s0140-6736(75)91074-0. [DOI] [PubMed] [Google Scholar]
  10. Cunningham D. A., Pattison J. R., Craig R. K. Detection of parvovirus DNA in human serum using biotinylated RNA hybridisation probes. J Virol Methods. 1988 Mar-Apr;19(3-4):279–288. doi: 10.1016/0166-0934(88)90022-5. [DOI] [PubMed] [Google Scholar]
  11. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  12. Gentilomi G., Musiani M., Zerbini M., Gallinella G., Gibellini D., La Placa M. A hybrido-immunocytochemical assay for the in situ detection of cytomegalovirus DNA using digoxigenin-labeled probes. J Immunol Methods. 1989 Dec 20;125(1-2):177–183. doi: 10.1016/0022-1759(89)90091-4. [DOI] [PubMed] [Google Scholar]
  13. Kurtzman G. J., Cohen B., Meyers P., Amunullah A., Young N. S. Persistent B19 parvovirus infection as a cause of severe chronic anaemia in children with acute lymphocytic leukaemia. Lancet. 1988 Nov 19;2(8621):1159–1162. doi: 10.1016/s0140-6736(88)90233-4. [DOI] [PubMed] [Google Scholar]
  14. Kurtzman G. J., Ozawa K., Cohen B., Hanson G., Oseas R., Young N. S. Chronic bone marrow failure due to persistent B19 parvovirus infection. N Engl J Med. 1987 Jul 30;317(5):287–294. doi: 10.1056/NEJM198707303170506. [DOI] [PubMed] [Google Scholar]
  15. Kurtzman G., Frickhofen N., Kimball J., Jenkins D. W., Nienhuis A. W., Young N. S. Pure red-cell aplasia of 10 years' duration due to persistent parvovirus B19 infection and its cure with immunoglobulin therapy. N Engl J Med. 1989 Aug 24;321(8):519–523. doi: 10.1056/NEJM198908243210807. [DOI] [PubMed] [Google Scholar]
  16. Mason W. S., Aldrich C., Summers J., Taylor J. M. Asymmetric replication of duck hepatitis B virus DNA in liver cells: Free minus-strand DNA. Proc Natl Acad Sci U S A. 1982 Jul;79(13):3997–4001. doi: 10.1073/pnas.79.13.3997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mori J., Field A. M., Clewley J. P., Cohen B. J. Dot blot hybridization assay of B19 virus DNA in clinical specimens. J Clin Microbiol. 1989 Mar;27(3):459–464. doi: 10.1128/jcm.27.3.459-464.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Ozawa K., Kurtzman G., Young N. Replication of the B19 parvovirus in human bone marrow cell cultures. Science. 1986 Aug 22;233(4766):883–886. doi: 10.1126/science.3738514. [DOI] [PubMed] [Google Scholar]
  19. Shneerson J. M., Mortimer P. P., Vandervelde E. M. Febrile illness due to a parvovirus. Br Med J. 1980 Jun 28;280(6231):1580–1580. doi: 10.1136/bmj.280.6231.1580. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES