Abstract
The availability of highly purified lipoarabinomannan from Mycobacterium tuberculosis in its native acylated, highly antigenic state allowed its application to the serodiagnosis of tuberculosis in patients from the Republic of Mexico. Antilipoarabinomannan immunoglobulin G antibodies in sera from 66 patients with pulmonary, miliary, and pleural tuberculosis and tuberculosis lymphadenities were measured by using the enzyme-linked immunosorbent assay against sera from a control population of healthy individuals, people with histoplasmosis, and people with lung diseases not caused by mycobacteria. The results pointed to an unexpectedly high degree of specificity of 91% and a sensitivity of 72%, comparable to figures from previous studies with other purified antigens; most of the false-positive results were for patients with histoplasmosis. Thus, lipoarabinomannan of M. tuberculosis is a potentially useful antigen for the serodiagnosis of tuberculosis.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Balestrino E. A., Daniel T. M., de Latini M. D., Latini O. A., Ma Y., Scocozza J. B. Serodiagnosis of pulmonary tuberculosis in Argentina by enzyme-linked immunosorbent assay (ELISA) of IgG antibody to Mycobacterium tuberculosis antigen 5 and tuberculin purified protein derivative. Bull World Health Organ. 1984;62(5):755–761. [PMC free article] [PubMed] [Google Scholar]
- Berger H. W., Mejia E. Tuberculous pleurisy. Chest. 1973 Jan;63(1):88–92. doi: 10.1378/chest.63.1.88. [DOI] [PubMed] [Google Scholar]
- Daniel T. M., Debanne S. M. The serodiagnosis of tuberculosis and other mycobacterial diseases by enzyme-linked immunosorbent assay. Am Rev Respir Dis. 1987 May;135(5):1137–1151. doi: 10.1164/arrd.1987.135.5.1137. [DOI] [PubMed] [Google Scholar]
- Hunter S. W., Gaylord H., Brennan P. J. Structure and antigenicity of the phosphorylated lipopolysaccharide antigens from the leprosy and tubercle bacilli. J Biol Chem. 1986 Sep 15;261(26):12345–12351. [PubMed] [Google Scholar]
- Ivanyi J., Sinha S., Aston R., Cussell D., Keen M., Sengupta U. Definition of species specific and cross-reactive antigenic determinants of Mycobacterium leprae using monoclonal antibodies. Clin Exp Immunol. 1983 Jun;52(3):528–536. [PMC free article] [PubMed] [Google Scholar]
- Levis W. R., Meeker H. C., Schuller-Levis G., Sersen E., Brennan P. J., Fried P. Mycobacterial carbohydrate antigens for serological testing of patients with leprosy. J Infect Dis. 1987 Nov;156(5):763–769. doi: 10.1093/infdis/156.5.763. [DOI] [PubMed] [Google Scholar]
- Ma Y., Wang Y. M., Daniel T. M. Enzyme-linked immunosorbent assay using Mycobacterium tuberculosis antigen 5 for the diagnosis of pulmonary tuberculosis in China. Am Rev Respir Dis. 1986 Dec;134(6):1273–1275. doi: 10.1164/arrd.1986.134.5.1273. [DOI] [PubMed] [Google Scholar]
- Wheat J., French M. L., Kamel S., Tewari R. P. Evaluation of cross-reactions in Histoplasma capsulatum serologic tests. J Clin Microbiol. 1986 Mar;23(3):493–499. doi: 10.1128/jcm.23.3.493-499.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zeiss C. R., Kalish S. B., Erlich K. S., Levitz D., Metzger E., Radin R., Phair J. P. IgG antibody to purified protein derivative by enzyme-linked immunosorbent assay in the diagnosis of pulmonary tuberculosis. Am Rev Respir Dis. 1984 Nov;130(5):845–848. doi: 10.1164/arrd.1984.130.5.845. [DOI] [PubMed] [Google Scholar]