Abstract
A colony immunoblot method was developed for serotyping the capsular polysaccharides expressed by Staphylococcus aureus isolates. The method was rapid and specific and was performed with either polyclonal or monoclonal antibodies specific for each of the capsule types. S. aureus isolates were obtained from patients with toxic shock syndrome (TSS) or other staphylococcal infections and from asymptomatic women with vaginal colonization. Among the vaginal isolates of S. aureus, expression of the type 8 capsule was significantly (P less than 0.001) more frequent among strains that produced TSS toxin 1 (TSST-1) than it was among TSST-1-negative strains. In contrast, the frequency of type 8 capsule expression was similar among both TSST-1-positive and -negative strains of S. aureus from patients with nonvaginal TSS. When all vaginal and nonvaginal isolates were compared, TSST-1-negative S. aureus strains were equally distributed among the type 5 and 8 and nontypeable capsule groups, whereas TSST-1-positive strains were predominantly capsule type 8.
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- Albus A., Fournier J. M., Wolz C., Boutonnier A., Ranke M., Høiby N., Hochkeppel H., Döring G. Staphylococcus aureus capsular types and antibody response to lung infection in patients with cystic fibrosis. J Clin Microbiol. 1988 Dec;26(12):2505–2509. doi: 10.1128/jcm.26.12.2505-2509.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Arbeit R. D., Karakawa W. W., Vann W. F., Robbins J. B. Predominance of two newly described capsular polysaccharide types among clinical isolates of Staphylococcus aureus. Diagn Microbiol Infect Dis. 1984 Apr;2(2):85–91. doi: 10.1016/0732-8893(84)90002-6. [DOI] [PubMed] [Google Scholar]
- Barbour A. G. Vaginal isolates of Staphylococcus aureus associated with toxic shock syndrome. Infect Immun. 1981 Aug;33(2):442–449. doi: 10.1128/iai.33.2.442-449.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cohen M. L., Graves L. M., Hayes P. S., Gibson R. J., Rasheed J. K., Feeley J. C. Toxic shock syndrome: modification and comparison of methods for detecting marker proteins in Staphylococcus aureus. J Clin Microbiol. 1983 Aug;18(2):372–375. doi: 10.1128/jcm.18.2.372-375.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Crass B. A., Bergdoll M. S. Toxin involvement in toxic shock syndrome. J Infect Dis. 1986 May;153(5):918–926. doi: 10.1093/infdis/153.5.918. [DOI] [PubMed] [Google Scholar]
- Fournier J. M., Bouvet A., Boutonnier A., Audurier A., Goldstein F., Pierre J., Bure A., Lebrun L., Hochkeppel H. K. Predominance of capsular polysaccharide type 5 among oxacillin-resistant Staphylococcus aureus. J Clin Microbiol. 1987 Oct;25(10):1932–1933. doi: 10.1128/jcm.25.10.1932-1933.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Garbe P. L., Arko R. J., Reingold A. L., Graves L. M., Hayes P. S., Hightower A. W., Chandler F. W., Broome C. V. Staphylococcus aureus isolates from patients with nonmenstrual toxic shock syndrome. Evidence for additional toxins. JAMA. 1985 May 3;253(17):2538–2542. [PubMed] [Google Scholar]
- HALLANDER H. O. PRODUCTION OF LARGE QUANTITIES OF ENTEROTOXIN B AND OTHER STAPHYLOCOCCAL TOXINS ON SOLID MEDIA. Acta Pathol Microbiol Scand. 1965;63:299–305. doi: 10.1111/apm.1965.63.2.299. [DOI] [PubMed] [Google Scholar]
- Hochkeppel H. K., Braun D. G., Vischer W., Imm A., Sutter S., Staeubli U., Guggenheim R., Kaplan E. L., Boutonnier A., Fournier J. M. Serotyping and electron microscopy studies of Staphylococcus aureus clinical isolates with monoclonal antibodies to capsular polysaccharide types 5 and 8. J Clin Microbiol. 1987 Mar;25(3):526–530. doi: 10.1128/jcm.25.3.526-530.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Karakawa W. W., Fournier J. M., Vann W. F., Arbeit R., Schneerson R. S., Robbins J. B. Method for the serological typing of the capsular polysaccharides of Staphylococcus aureus. J Clin Microbiol. 1985 Sep;22(3):445–447. doi: 10.1128/jcm.22.3.445-447.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Musser J. M., Schlievert P. M., Chow A. W., Ewan P., Kreiswirth B. N., Rosdahl V. T., Naidu A. S., Witte W., Selander R. K. A single clone of Staphylococcus aureus causes the majority of cases of toxic shock syndrome. Proc Natl Acad Sci U S A. 1990 Jan;87(1):225–229. doi: 10.1073/pnas.87.1.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nelles M. J., Niswander C. A., Karakawa W. W., Vann W. F., Arbeit R. D. Reactivity of type-specific monoclonal antibodies with Staphylococcus aureus clinical isolates and purified capsular polysaccharide. Infect Immun. 1985 Jul;49(1):14–18. doi: 10.1128/iai.49.1.14-18.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- OUCHTERLONY O. Diffusion-in-gel methods for immunological analysis. Prog Allergy. 1958;5:1–78. [PubMed] [Google Scholar]
- Parsonnet J., Gillis Z. A., Pier G. B. Induction of interleukin-1 by strains of Staphylococcus aureus from patients with nonmenstrual toxic shock syndrome. J Infect Dis. 1986 Jul;154(1):55–63. doi: 10.1093/infdis/154.1.55. [DOI] [PubMed] [Google Scholar]
- Parsonnet J., Gillis Z. A., Richter A. G., Pier G. B. A rabbit model of toxic shock syndrome that uses a constant, subcutaneous infusion of toxic shock syndrome toxin 1. Infect Immun. 1987 May;55(5):1070–1076. doi: 10.1128/iai.55.5.1070-1076.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Parsonnet J., Mills J. T., Gillis Z. A., Pier G. B. Competitive, enzyme-linked immunosorbent assay for toxic shock syndrome toxin 1. J Clin Microbiol. 1985 Jul;22(1):26–31. doi: 10.1128/jcm.22.1.26-31.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Poutrel B., Boutonnier A., Sutra L., Fournier J. M. Prevalence of capsular polysaccharide types 5 and 8 among Staphylococcus aureus isolates from cow, goat, and ewe milk. J Clin Microbiol. 1988 Jan;26(1):38–40. doi: 10.1128/jcm.26.1.38-40.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reeves M. W., Pine L., Feeley J. C., Wells D. E. Presence of toxic shock toxin in toxic shock and other clinical strains of Staphylococcus aureus. Infect Immun. 1984 Nov;46(2):590–597. doi: 10.1128/iai.46.2.590-597.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reingold A. L., Hargrett N. T., Shands K. N., Dan B. B., Schmid G. P., Strickland B. Y., Broome C. V. Toxic shock syndrome surveillance in the United States, 1980 to 1981. Ann Intern Med. 1982 Jun;96(6 Pt 2):875–880. doi: 10.7326/0003-4819-96-6-875. [DOI] [PubMed] [Google Scholar]
- Schlievert P. M., Shands K. N., Dan B. B., Schmid G. P., Nishimura R. D. Identification and characterization of an exotoxin from Staphylococcus aureus associated with toxic-shock syndrome. J Infect Dis. 1981 Apr;143(4):509–516. doi: 10.1093/infdis/143.4.509. [DOI] [PubMed] [Google Scholar]
- Schlievert P. M. Staphylococcal enterotoxin B and toxic-shock syndrome toxin-1 are significantly associated with non-menstrual TSS. Lancet. 1986 May 17;1(8490):1149–1150. doi: 10.1016/s0140-6736(86)91859-3. [DOI] [PubMed] [Google Scholar]
- Sompolinsky D., Samra Z., Karakawa W. W., Vann W. F., Schneerson R., Malik Z. Encapsulation and capsular types in isolates of Staphylococcus aureus from different sources and relationship to phage types. J Clin Microbiol. 1985 Nov;22(5):828–834. doi: 10.1128/jcm.22.5.828-834.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Staneck J. L., Bonventre P. F. Toxic shock syndrome: a microbiological perspective. Surv Synth Pathol Res. 1984;3(1):38–53. doi: 10.1159/000156914. [DOI] [PubMed] [Google Scholar]
- Todd J. K., Franco-Buff A., Lawellin D. W., Vasil M. L. Phenotypic distinctiveness of Staphylococcus aureus strains associated with toxic shock syndrome. Infect Immun. 1984 Aug;45(2):339–344. doi: 10.1128/iai.45.2.339-344.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Van Miert A. S., Van Duin C. T., Schotman A. J. Comparative observations of fever and associated clinical hematological and blood biochemical changes after intravenous administration of staphylococcal enterotoxins B and F (toxic shock syndrome toxin-1) in goats. Infect Immun. 1984 Nov;46(2):354–360. doi: 10.1128/iai.46.2.354-360.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]