Abstract
A rapid high-performance liquid chromatography method was developed to determine hippurate hydrolysis by Legionella spp. Benzoic acid, an end product of enzymatic activity, was directly detected by high-performance liquid chromatography after 1 and 24 h of incubation in 1% sodium hippurate. Because of its sensitivity, this procedure offers more precise identification of some Legionella spp.
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Selected References
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- Brenner D. J., Steigerwalt A. G., Epple P., Bibb W. F., McKinney R. M., Starnes R. W., Colville J. M., Selander R. K., Edelstein P. H., Moss C. W. Legionella pneumophila serogroup Lansing 3 isolated from a patient with fatal pneumonia, and descriptions of L. pneumophila subsp. pneumophila subsp. nov., L. pneumophila subsp. fraseri subsp. nov., and L. pneumophila subsp. pascullei subsp. nov. J Clin Microbiol. 1988 Sep;26(9):1695–1703. doi: 10.1128/jcm.26.9.1695-1703.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Edberg S. C., Samuels S. Rapid, colorimetric test for the determination of hippurate hydrolysis by group B Streptococcus. J Clin Microbiol. 1976 Jan;3(1):49–50. doi: 10.1128/jcm.3.1.49-50.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hwang M. N., Ederer G. M. Rapid hippurate hydrolysis method for presumptive identification of group B streptococci. J Clin Microbiol. 1975 Jan;1(1):114–115. doi: 10.1128/jcm.1.1.114-115.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hébert G. A. Hippurate hydrolysis by Legionella pneumophila. J Clin Microbiol. 1981 Jan;13(1):240–242. doi: 10.1128/jcm.13.1.240-242.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kubota K., Horai Y., Kushida K., Ishizaki T. Determination of benzoic acid and hippuric acid in human plasma and urine by high-performance liquid chromatography. J Chromatogr. 1988 Mar 4;425(1):67–75. doi: 10.1016/0378-4347(88)80007-0. [DOI] [PubMed] [Google Scholar]
- Wallace P. L., Patton C. M., Moss C. W. Determination of hippurate hydrolysis by gas-liquid chromatography. J Clin Microbiol. 1987 Sep;25(9):1766–1768. doi: 10.1128/jcm.25.9.1766-1768.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]