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. 1990 Dec;28(12):2837–2841. doi: 10.1128/jcm.28.12.2837-2841.1990

Antigenic and genetic analyses of human rotavirus with dual subgroup specificity.

T Urasawa 1, K Taniguchi 1, N Kobayashi 1, F Wakasugi 1, I Oishi 1, Y Minekawa 1, M Oseto 1, M U Ahmed 1, S Urasawa 1
PMCID: PMC268290  PMID: 2177756

Abstract

In our previous study (S. Urasawa, T. Urasawa, K. Taniguchi, F. Wakasugi, N. Kobayashi, S. Chiba, N. Sakurada, S. Morita, O. Morita, M. Tokieda, T. Kawamoto, K. Minekawa, and M. Oseto, J. Infect. Dis. 160:44-51, 1989) of antigenic characterization of about 300 human rotavirus (HRV) isolates collected at different localities in Japan, we found 4 HRV isolates having unique antigenic and genetic constructions. The four strains possessed both subgroup I and subgroup II antigens, serotype 3 antigen, and a long RNA electropherotype. The reactivity pattern of these four HRV isolates with three monoclonal antibodies (MAbs) directed to an outer capsid protein, VP4, and with one MAb directed to an inner capsid protein, VP2, was clearly different from those of usual subgroup II HRVs having serotype 1, serotype 3, or serotype 4 specificity and a long RNA pattern, whereas their reactivity pattern was similar to that of strain K8 (subgroup II, serotype 1), which possessed unique VP4 and VP2 proteins. RNA-RNA cross-hybridization analysis indicated that while the four isolates were genetically distinct from the two genetic groups of HRV reported previously, i.e., the Wa family (strains KU, S3, and YO) and the DS-1 family (strain S2), they were closely related to strain K8, a strain having unique antigenic and genetic properties (K. Taniguchi, K. Nishikawa, T. Urasawa, S. Urasawa, K. Midthun, A. Z. Kapikian, and M. Gorziglia, J. Virol. 63:4101-4106, 1989).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aboudy Y., Shif I., Zilberstein I., Gotlieb-Stematsky T. Use of polyclonal and monoclonal antibodies and analysis of viral RNA in the detection of unusual group A human rotaviruses. J Med Virol. 1988 Jul;25(3):351–359. doi: 10.1002/jmv.1890250312. [DOI] [PubMed] [Google Scholar]
  2. Clark H. F., Hoshino Y., Bell L. M., Groff J., Hess G., Bachman P., Offit P. A. Rotavirus isolate WI61 representing a presumptive new human serotype. J Clin Microbiol. 1987 Sep;25(9):1757–1762. doi: 10.1128/jcm.25.9.1757-1762.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Espejo R. T., López S., Arias C. Structural polypeptides of simian rotavirus SA11 and the effect of trypsin. J Virol. 1981 Jan;37(1):156–160. doi: 10.1128/jvi.37.1.156-160.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Estes M. K., Graham D. Y., Mason B. B. Proteolytic enhancement of rotavirus infectivity: molecular mechanisms. J Virol. 1981 Sep;39(3):879–888. doi: 10.1128/jvi.39.3.879-888.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Flores J., Perez I., White L., Perez M., Kalica A. R., Marquina R., Wyatt R. G., Kapikian A. Z., Chanock R. M. Genetic relatedness among human rotaviruses as determined by RNA hybridization. Infect Immun. 1982 Aug;37(2):648–655. doi: 10.1128/iai.37.2.648-655.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ghosh S. K., Naik T. N. Detection of a large number of subgroup 1 human rotaviruses with a "long" RNA electropherotype. Arch Virol. 1989;105(1-2):119–127. doi: 10.1007/BF01311122. [DOI] [PubMed] [Google Scholar]
  7. Gorziglia M., Green K., Nishikawa K., Taniguchi K., Jones R., Kapikian A. Z., Chanock R. M. Sequence of the fourth gene of human rotaviruses recovered from asymptomatic or symptomatic infections. J Virol. 1988 Aug;62(8):2978–2984. doi: 10.1128/jvi.62.8.2978-2984.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gorziglia M., Hoshino Y., Buckler-White A., Blumentals I., Glass R., Flores J., Kapikian A. Z., Chanock R. M. Conservation of amino acid sequence of VP8 and cleavage region of 84-kDa outer capsid protein among rotaviruses recovered from asymptomatic neonatal infection. Proc Natl Acad Sci U S A. 1986 Sep;83(18):7039–7043. doi: 10.1073/pnas.83.18.7039. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Greenberg H. B., Flores J., Kalica A. R., Wyatt R. G., Jones R. Gene coding assignments for growth restriction, neutralization and subgroup specificities of the W and DS-1 strains of human rotavirus. J Gen Virol. 1983 Feb;64(Pt 2):313–320. doi: 10.1099/0022-1317-64-2-313. [DOI] [PubMed] [Google Scholar]
  10. Herring A. J., Inglis N. F., Ojeh C. K., Snodgrass D. R., Menzies J. D. Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels. J Clin Microbiol. 1982 Sep;16(3):473–477. doi: 10.1128/jcm.16.3.473-477.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hoshino Y., Gorziglia M., Valdesuso J., Askaa J., Glass R. I., Kapikian A. Z. An equine rotavirus (FI-14 strain) which bears both subgroup I and subgroup II specificities on its VP6. Virology. 1987 Apr;157(2):488–496. doi: 10.1016/0042-6822(87)90291-1. [DOI] [PubMed] [Google Scholar]
  12. Kalica A. R., Flores J., Greenberg H. B. Identification of the rotaviral gene that codes for hemagglutination and protease-enhanced plaque formation. Virology. 1983 Feb;125(1):194–205. doi: 10.1016/0042-6822(83)90073-9. [DOI] [PubMed] [Google Scholar]
  13. Kalica A. R., Greenberg H. B., Espejo R. T., Flores J., Wyatt R. G., Kapikian A. Z., Chanock R. M. Distinctive ribonucleic acid patterns of human rotavirus subgroups 1 and 2. Infect Immun. 1981 Sep;33(3):958–961. doi: 10.1128/iai.33.3.958-961.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kalica A. R., Greenberg H. B., Wyatt R. G., Flores J., Sereno M. M., Kapikian A. Z., Chanock R. M. Genes of human (strain Wa) and bovine (strain UK) rotaviruses that code for neutralization and subgroup antigens. Virology. 1981 Jul 30;112(2):385–390. doi: 10.1016/0042-6822(81)90285-3. [DOI] [PubMed] [Google Scholar]
  15. Kantharidis P., Dyall-Smith M. L., Holmes I. H. Marked sequence variation between segment 4 genes of human RV-5 and simian SA 11 rotaviruses. Arch Virol. 1987;93(1-2):111–121. doi: 10.1007/BF01313897. [DOI] [PubMed] [Google Scholar]
  16. Kobayashi N., Lintag I. C., Urasawa T., Taniguchi K., Saniel M. C., Urasawa S. Unusual human rotavirus strains having subgroup I specificity and "long" RNA electropherotype. Arch Virol. 1989;109(1-2):11–23. doi: 10.1007/BF01310514. [DOI] [PubMed] [Google Scholar]
  17. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  18. Matsuno S., Hasegawa A., Mukoyama A., Inouye S. A candidate for a new serotype of human rotavirus. J Virol. 1985 May;54(2):623–624. doi: 10.1128/jvi.54.2.623-624.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. McCrae M. A., McCorquodale J. G. The molecular biology of rotaviruses. II. Identification of the protein-coding assignments of calf rotavirus genome RNA species. Virology. 1982 Mar;117(2):435–443. doi: 10.1016/0042-6822(82)90482-2. [DOI] [PubMed] [Google Scholar]
  20. Midthun K., Valdesuso J., Hoshino Y., Flores J., Kapikian A. Z., Chanock R. M. Analysis by RNA-RNA hybridization assay of intertypic rotaviruses suggests that gene reassortment occurs in vivo. J Clin Microbiol. 1987 Feb;25(2):295–300. doi: 10.1128/jcm.25.2.295-300.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Nakagomi O., Nakagomi T., Hoshino Y., Flores J., Kapikian A. Z. Genetic analysis of a human rotavirus that belongs to subgroup I but has an RNA pattern typical of subgroup II human rotaviruses. J Clin Microbiol. 1987 Jul;25(7):1159–1164. doi: 10.1128/jcm.25.7.1159-1164.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Nakagomi T., Nakagomi O. RNA-RNA hybridization identifies a human rotavirus that is genetically related to feline rotavirus. J Virol. 1989 Mar;63(3):1431–1434. doi: 10.1128/jvi.63.3.1431-1434.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Pedley S., Bridger J. C., Brown J. F., McCrae M. A. Molecular characterization of rotaviruses with distinct group antigens. J Gen Virol. 1983 Oct;64(Pt 10):2093–2101. doi: 10.1099/0022-1317-64-10-2093. [DOI] [PubMed] [Google Scholar]
  24. Taniguchi K., Maloy W. L., Nishikawa K., Green K. Y., Hoshino Y., Urasawa S., Kapikian A. Z., Chanock R. M., Gorziglia M. Identification of cross-reactive and serotype 2-specific neutralization epitopes on VP3 of human rotavirus. J Virol. 1988 Jul;62(7):2421–2426. doi: 10.1128/jvi.62.7.2421-2426.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Taniguchi K., Morita Y., Urasawa T., Urasawa S. Cross-reactive neutralization epitopes on VP3 of human rotavirus: analysis with monoclonal antibodies and antigenic variants. J Virol. 1987 May;61(5):1726–1730. doi: 10.1128/jvi.61.5.1726-1730.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Taniguchi K., Nishikawa K., Urasawa T., Urasawa S., Midthun K., Kapikian A. Z., Gorziglia M. Complete nucleotide sequence of the gene encoding VP4 of a human rotavirus (strain K8) which has unique VP4 neutralization epitopes. J Virol. 1989 Sep;63(9):4101–4106. doi: 10.1128/jvi.63.9.4101-4106.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Taniguchi K., Urasawa S., Urasawa T. Electrophoretic analysis of RNA segments of human rotaviruses cultivated in cell culture. J Gen Virol. 1982 May;60(Pt 1):171–175. doi: 10.1099/0022-1317-60-1-171. [DOI] [PubMed] [Google Scholar]
  28. Taniguchi K., Urasawa S., Urasawa T. Preparation and characterization of neutralizing monoclonal antibodies with different reactivity patterns to human rotaviruses. J Gen Virol. 1985 May;66(Pt 5):1045–1053. doi: 10.1099/0022-1317-66-5-1045. [DOI] [PubMed] [Google Scholar]
  29. Taniguchi K., Urasawa T., Morita Y., Greenberg H. B., Urasawa S. Direct serotyping of human rotavirus in stools by an enzyme-linked immunosorbent assay using serotype 1-, 2-, 3-, and 4-specific monoclonal antibodies to VP7. J Infect Dis. 1987 Jun;155(6):1159–1166. doi: 10.1093/infdis/155.6.1159. [DOI] [PubMed] [Google Scholar]
  30. Taniguchi K., Urasawa T., Urasawa S. Reactivity patterns to human rotavirus strains of a monoclonal antibody against VP2, a component of the inner capsid of rotavirus. Brief report. Arch Virol. 1986;87(1-2):135–141. doi: 10.1007/BF01310550. [DOI] [PubMed] [Google Scholar]
  31. Taniguchi K., Urasawa T., Urasawa S., Yasuhara T. Production of subgroup-specific monoclonal antibodies against human rotaviruses and their application to an enzyme-linked immunosorbent assay for subgroup determination. J Med Virol. 1984;14(2):115–125. doi: 10.1002/jmv.1890140205. [DOI] [PubMed] [Google Scholar]
  32. Urasawa S., Urasawa T., Taniguchi K., Morita Y., Sakurada N., Saeki Y., Morita O., Hasegawa S. Validity of an enzyme-linked immunosorbent assay with serotype-specific monoclonal antibodies for serotyping human rotavirus in stool specimens. Microbiol Immunol. 1988;32(7):699–708. doi: 10.1111/j.1348-0421.1988.tb01431.x. [DOI] [PubMed] [Google Scholar]
  33. Urasawa S., Urasawa T., Taniguchi K., Wakasugi F., Kobayashi N., Chiba S., Sakurada N., Morita M., Morita O., Tokieda M. Survey of human rotavirus serotypes in different locales in Japan by enzyme-linked immunosorbent assay with monoclonal antibodies. J Infect Dis. 1989 Jul;160(1):44–51. doi: 10.1093/infdis/160.1.44. [DOI] [PubMed] [Google Scholar]
  34. Urasawa T., Urasawa S., Taniguchi K. Sequential passages of human rotavirus in MA-104 cells. Microbiol Immunol. 1981;25(10):1025–1035. doi: 10.1111/j.1348-0421.1981.tb00109.x. [DOI] [PubMed] [Google Scholar]
  35. Wyatt R. G., James H. D., Jr, Pittman A. L., Hoshino Y., Greenberg H. B., Kalica A. R., Flores J., Kapikian A. Z. Direct isolation in cell culture of human rotaviruses and their characterization into four serotypes. J Clin Microbiol. 1983 Aug;18(2):310–317. doi: 10.1128/jcm.18.2.310-317.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]

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