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. 1985 Jul;22(1):1–4. doi: 10.1128/jcm.22.1.1-4.1985

Second serogroup of Legionella feeleii strains isolated from humans.

W L Thacker, H W Wilkinson, B B Plikaytis, A G Steigerwalt, W R Mayberry, C W Moss, D J Brenner
PMCID: PMC268308  PMID: 3894411

Abstract

Three strains of Legionella feeleii from patients with pneumonia (425-MI-H, 691-WI-H, and 693-WI-H) and one environmental strain (713-MI-E) received at the Centers for Disease Control for reference diagnostic testing were compared with the type strain WO-44C-C3 (ATCC 35072) by DNA hybridization, chemical analysis of cellular fatty acids and ubiquinones, biochemical tests, and serological characteristics. All four isolates were assigned to the L. feeleii species on the basis of DNA hybridization results. However, strains 691-WI-H and 693-WI-H were serologically distinct from strain WO-44C-C3, as shown by their minimal reactivity (1 to 2+) with a direct immunofluorescence conjugate prepared against L. feeleii serogroup 1 (strain WO-44C-C3). Therefore, strains 691-WI-H and 693-WI-H were placed in a new L. feeleii serogroup (serogroup 2). The reference strain of L. feeleii serogroup 2 is 691-WI-H (ATCC 35849).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brenner D. J., McWhorter A. C., Knutson J. K., Steigerwalt A. G. Escherichia vulneris: a new species of Enterobacteriaceae associated with human wounds. J Clin Microbiol. 1982 Jun;15(6):1133–1140. doi: 10.1128/jcm.15.6.1133-1140.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brown S. L., Bibb W. F., McKinney R. M. Retrospective examination of lung tissue specimens for the presence of Legionella organisms: comparison of an indirect fluorescent-antibody system with direct fluorescent-antibody testing. J Clin Microbiol. 1984 Apr;19(4):468–472. doi: 10.1128/jcm.19.4.468-472.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Feeley J. C., Gibson R. J., Gorman G. W., Langford N. C., Rasheed J. K., Mackel D. C., Baine W. B. Charcoal-yeast extract agar: primary isolation medium for Legionella pneumophila. J Clin Microbiol. 1979 Oct;10(4):437–441. doi: 10.1128/jcm.10.4.437-441.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Herwaldt L. A., Gorman G. W., McGrath T., Toma S., Brake B., Hightower A. W., Jones J., Reingold A. L., Boxer P. A., Tang P. W. A new Legionella species, Legionella feeleii species nova, causes Pontiac fever in an automobile plant. Ann Intern Med. 1984 Mar;100(3):333–338. doi: 10.7326/0003-4819-100-3-333. [DOI] [PubMed] [Google Scholar]
  5. Kodaka H., Armfield A. Y., Lombard G. L., Dowell V. R., Jr Practical procedure for demonstrating bacterial flagella. J Clin Microbiol. 1982 Nov;16(5):948–952. doi: 10.1128/jcm.16.5.948-952.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Moss C. W., Bibb W. F., Karr D. E., Guerrant G. O., Lambert M. A. Cellular fatty acid composition and ubiquinone content of Legionella feeleii sp. nov. J Clin Microbiol. 1983 Oct;18(4):917–919. doi: 10.1128/jcm.18.4.917-919.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Moss C. W. Gas-liquid chromatography as an analytical tool in microbiology. J Chromatogr. 1981 Jan 9;203:337–347. doi: 10.1016/s0021-9673(00)80305-2. [DOI] [PubMed] [Google Scholar]
  8. Moss C. W., Guerrant G. O. Separation of bacterial ubiquinones by reverse-phase high-pressure liquid chromatography. J Clin Microbiol. 1983 Jul;18(1):15–17. doi: 10.1128/jcm.18.1.15-17.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Orrison L. H., Cherry W. B., Tyndall R. L., Fliermans C. B., Gough S. B., Lambert M. A., McDougal L. K., Bibb W. F., Brenner D. J. Legionella oakridgensis: unusual new species isolated from cooling tower water. Appl Environ Microbiol. 1983 Feb;45(2):536–545. doi: 10.1128/aem.45.2.536-545.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Pasculle A. W., Feeley J. C., Gibson R. J., Cordes L. G., Myerowitz R. L., Patton C. M., Gorman G. W., Carmack C. L., Ezzell J. W., Dowling J. N. Pittsburgh pneumonia agent: direct isolation from human lung tissue. J Infect Dis. 1980 Jun;141(6):727–732. doi: 10.1093/infdis/141.6.727. [DOI] [PubMed] [Google Scholar]
  11. Thacker W. L., Plikaytis B. B., Wilkinson H. W. Identification of 22 Legionella species and 33 serogroups with the slide agglutination test. J Clin Microbiol. 1985 May;21(5):779–782. doi: 10.1128/jcm.21.5.779-782.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Thacker W. L., Wilkinson H. W., Benson R. F. Comparison of slide agglutination test and direct immunofluorescence assay for identification of Legionella isolates. J Clin Microbiol. 1983 Nov;18(5):1113–1118. doi: 10.1128/jcm.18.5.1113-1118.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wilkinson H. W., Fikes B. J., Cruce D. D. Indirect immunofluorescence test for serodiagnosis of Legionnaires disease: evidence for serogroup diversity of Legionnaires disease bacterial antigens and for multiple specificity of human antibodies. J Clin Microbiol. 1979 Mar;9(3):379–383. doi: 10.1128/jcm.9.3.379-383.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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