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. 1985 Jul;22(1):119–124. doi: 10.1128/jcm.22.1.119-124.1985

Detection of hepatitis A virus and antibody by solid-phase radioimmunoassay and enzyme-linked immunosorbent assay with monoclonal antibodies.

A G Coulepis, M F Veale, A MacGregor, M Kornitschuk, I D Gust
PMCID: PMC268334  PMID: 2991329

Abstract

Monoclonal antibodies (K3-2F2 and K3-4C8) raised against hepatitis A virus were used to develop a solid-phase radioimmunoassay and enzyme-linked immunosorbent assay for the detection of hepatitis A virus and antibody. Assays with this pair of monoclonal antibodies were compared in parallel with similarly constructed solid-phase radioimmunoassays and enzyme-linked immunosorbent assays in which human polyclonal serum was used. The monoclonal antibody assay proved to be more sensitive for the detection of hepatitis A virus from fecal specimens as well as for anti-hepatitis A virus immunoglobulin G (IgG) and IgM in sera.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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