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. 1985 Aug;22(2):145–148. doi: 10.1128/jcm.22.2.145-148.1985

Evaluation of Detect-A-Strep and the Culturette Ten-Minute Strep ID kits for detection of group A streptococcal antigen in oropharyngeal swabs from children.

J M Campos, C C Charilaou
PMCID: PMC268347  PMID: 3897261

Abstract

The Detect-A-Strep kit (DAS) (Antibodies Inc.) and the Culturette Brand 10-Minute Group A Strep ID kit (CBTMSI) (Marion Scientific) were compared with a sensitive culture method for detection of group A streptococci in oropharyngeal swabs from children. Specimens from 953 children ranging in age from 4 months to 18 years were inoculated to selective blood agar plates containing sulfamethoxazole-trimethoprim. Culture plates were incubated anaerobically for the first overnight period and aerobically in the presence of 10% CO2 for the second overnight period. The same specimens were tested within 24 h by DAS (phase 1) or CBTMSI (phase 2). One hundred thirty-five of 538 specimens (25.1%) tested by DAS and 150 of 415 specimens (36.1%) tested by CBTMSI were culture positive. DAS detected 87 of 135 (64.4%) and CBTMSI detected 93 of 150 (62.0%) culture-positive specimens. The specificities of DAS and CBTMSI were 96.5 and 99.6%, respectively. The predictive values of positive and negative results were 86.1 and 89.0% for DAS and 98.9 and 82.2% for CBTMSI. Because a reliable distinction between infected patients and carrier state patients cannot be made, we conclude that neither DAS nor CBTMSI is sufficiently sensitive to replace our culture method.

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Selected References

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  1. Dykstra M. A., McLaughlin J. C., Bartlett R. C. Comparison of media and techniques for detection of group A streptococci in throat swab specimens. J Clin Microbiol. 1979 Feb;9(2):236–238. doi: 10.1128/jcm.9.2.236-238.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Edwards E. A., Phillips I. A., Suiter W. C. Diagnosis of group A streptococcal infections directly from throat secretions. J Clin Microbiol. 1982 Mar;15(3):481–483. doi: 10.1128/jcm.15.3.481-483.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gerber M. A. Micronitrous acid extraction-coagglutination test for rapid diagnosis of streptococcal pharyngitis. J Clin Microbiol. 1983 Jan;17(1):170–171. doi: 10.1128/jcm.17.1.170-171.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gerber M. A., Spadaccini L. J., Wright L. L., Deutsch L. Latex agglutination tests for rapid identification of group A streptococci directly from throat swabs. J Pediatr. 1984 Nov;105(5):702–705. doi: 10.1016/s0022-3476(84)80286-3. [DOI] [PubMed] [Google Scholar]
  5. Gordis L. Effectiveness of comprehensive-care programs in preventing rheumatic fever. N Engl J Med. 1973 Aug 16;289(7):331–335. doi: 10.1056/NEJM197308162890701. [DOI] [PubMed] [Google Scholar]
  6. Gunn B. A., Ohashi D. K., Gaydos C. A., Holt E. S. Selective and enhanced recovery of group A and B streptococci from throat cultures with sheep blood agar containing sulfamethoxazole and trimethoprim. J Clin Microbiol. 1977 Jun;5(6):650–655. doi: 10.1128/jcm.5.6.650-655.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kaplan E. L., Top F. H., Jr, Dudding B. A., Wannamaker L. W. Diagnosis of streptococcal pharyngitis: differentiation of active infection from the carrier state in the symptomatic child. J Infect Dis. 1971 May;123(5):490–501. doi: 10.1093/infdis/123.5.490. [DOI] [PubMed] [Google Scholar]
  8. Kholy A. E., Facklam R., Sabri G., Rotta J. Serological identification of group A streptococci from throat scrapings before culture. J Clin Microbiol. 1978 Dec;8(6):725–728. doi: 10.1128/jcm.8.6.725-728.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kurzynski T. A., Meise C. K. Evaluation of sulfamethoxazole-trimethoprim blood agar plates for recovery of group A streptocci from throat cultures. J Clin Microbiol. 1979 Feb;9(2):189–193. doi: 10.1128/jcm.9.2.189-193.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kurzynski T. A., Van Holten C. M. Evaluation of techniques for isolation of group A streptococci from throat cultures. J Clin Microbiol. 1981 May;13(5):891–894. doi: 10.1128/jcm.13.5.891-894.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lauer B. A., Reller L. B., Mirrett S. Effect of atmosphere and duration of incubation on primary isolation of group A streptococci from throat cultures. J Clin Microbiol. 1983 Feb;17(2):338–340. doi: 10.1128/jcm.17.2.338-340.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. McCusker J. J., McCoy E. L., Young C. L., Alamares R., Hirsch L. S. Comparison of Directigen Group A Strep Test with a traditional culture technique for detection of group A beta-hemolytic streptococci. J Clin Microbiol. 1984 Oct;20(4):824–825. doi: 10.1128/jcm.20.4.824-825.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Miller J. M., Phillips H. L., Graves R. K., Facklam R. R. Evaluation of the Directigen Group A Strep test kit. J Clin Microbiol. 1984 Nov;20(5):846–848. doi: 10.1128/jcm.20.5.846-848.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Murray P. R., Wold A. D., Schreck C. A., Washington JA I. I. Effects of selective media and atmosphere of incubation on the isolation of group A streptococci. J Clin Microbiol. 1976 Jul;4(1):54–56. doi: 10.1128/jcm.4.1.54-56.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Otero J. R., Reyes S., Noriega A. R. Rapid diagnosis of group A streptococcal antigen extracted directly from swabs by an enzymatic procedure and used to detect pharyngitis. J Clin Microbiol. 1983 Aug;18(2):318–320. doi: 10.1128/jcm.18.2.318-320.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Petts D. N. Early detection of streptococci in swabs by latex agglutination before culture. J Clin Microbiol. 1984 Mar;19(3):432–433. doi: 10.1128/jcm.19.3.432-433.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Slifkin M., Gil G. M. Evaluation of the Culturette Brand Ten-Minute Group A Strep ID technique. J Clin Microbiol. 1984 Jul;20(1):12–14. doi: 10.1128/jcm.20.1.12-14.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Slifkin M., Gil G. M. Serogrouping of beta-hemolytic streptococci from throat swabs with nitrous acid extraction and the Phadebact streptococcus test. J Clin Microbiol. 1982 Jan;15(1):187–189. doi: 10.1128/jcm.15.1.187-189.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

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