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. 1985 Aug;22(2):212–215. doi: 10.1128/jcm.22.2.212-215.1985

Antimicrobial susceptibility testing of Francisella tularensis with a modified Mueller-Hinton broth.

C N Baker, D G Hollis, C Thornsberry
PMCID: PMC268361  PMID: 4031036

Abstract

A modified Mueller-Hinton broth was developed to perform antimicrobial susceptibility tests on Francisella tularensis. Adequate growth of the organism was obtained within 24 h of inoculation, and MICs could be read at that time. We tested 15 selected strains of F. tularensis and five reference quality control strains in this medium with 36 antimicrobial agents. The MICs of the aminoglycosides and tetracycline increased 1 to 3 dilutions in this medium compared with those in the usual medium, but the other antimicrobial agents were not consistently affected by the medium. Even though the medium caused an increase in MICs, the aminoglycosides and tetracyclines remained very active in vitro against F. tularensis. Other antimicrobial agents effective in vitro were chloramphenicol, erythromycin, ceftazidime, moxalactam, cefotaxime, ceftriaxone, and Sch 29482 (a cephalosporin).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BIEGELEISEN J. Z., Jr, MOODY M. D. Sensitivity in vitro of eighteen strains of Pasteurelia tularensis to erythromycin. J Bacteriol. 1960 Jan;79:155–156. doi: 10.1128/jb.79.1.155-156.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berdal B. P., Soderlund E. Cultivation and isolation of Francisella tularensis on selective chocolate agar, as used routinely for the isolation of gonoccocci. Acta Pathol Microbiol Scand B. 1977 Feb;85B(1):108–109. doi: 10.1111/j.1699-0463.1977.tb01681.x. [DOI] [PubMed] [Google Scholar]
  3. CORWIN W. C., STUBBS S. P. Further studies on tularemia in the Ozarks; review of forty-four cases during a three-year period. J Am Med Assoc. 1952 May 24;149(4):343–345. doi: 10.1001/jama.1952.02930210027007. [DOI] [PubMed] [Google Scholar]
  4. EIGELSBACH H. T., BRAUN W., HERRING R. D. Studies on the variation of Bacterium tularense. J Bacteriol. 1951 May;61(5):557–569. doi: 10.1128/jb.61.5.557-569.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Feeley J. C., Gibson R. J., Gorman G. W., Langford N. C., Rasheed J. K., Mackel D. C., Baine W. B. Charcoal-yeast extract agar: primary isolation medium for Legionella pneumophila. J Clin Microbiol. 1979 Oct;10(4):437–441. doi: 10.1128/jcm.10.4.437-441.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Francis E. Fermentation of Sugars by Bacterium Tularense. J Bacteriol. 1942 Mar;43(3):343–346. doi: 10.1128/jb.43.3.343-346.1942. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hesselbrock W., Foshay L. The Morphology of Bacterium tularense. J Bacteriol. 1945 Mar;49(3):209–231. doi: 10.1128/jb.49.3.209-231.1945. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kudelina R. I., Olsufiev N. G. Sensitivity to macrolide antibiotics and lincomycin in Francisella tularensis holarctica. J Hyg Epidemiol Microbiol Immunol. 1980;24(1):84–91. [PubMed] [Google Scholar]
  9. Martone W. J., Marshall L. W., Kaufmann A. F., Hobbs J. H., Levy M. E. Tularemia pneumonia in Washington, DC. A report of three cases with possible common-source exposures. JAMA. 1979 Nov 23;242(21):2315–2317. doi: 10.1001/jama.242.21.2315. [DOI] [PubMed] [Google Scholar]
  10. Mason W. L., Eigelsbach H. T., Little S. F., Bates J. H. Treatment of tularemia, including pulmonary tularemia, with gentamicin. Am Rev Respir Dis. 1980 Jan;121(1):39–45. doi: 10.1164/arrd.1980.121.1.39. [DOI] [PubMed] [Google Scholar]
  11. Miller L. G. Further studies on tularemia in Alaska: virulence and biochemical characteristics of indigenous strains. Can J Microbiol. 1974 Nov;20(11):1585–1590. doi: 10.1139/m74-245. [DOI] [PubMed] [Google Scholar]
  12. Münnich D., Lakatos M. Clinical, epidemiological and therapeutical experience with human tularaemia: the role of hamster hunters. Infection. 1979;7(2):61–63. doi: 10.1007/BF01641614. [DOI] [PubMed] [Google Scholar]
  13. OVERHOLT E. L., TIGERTT W. D., KADULL P. J., WARD M. K., CHARKES N. D., RENE R. M., SALZMAN T. E., STEPHENS M. An analysis of forty-two cases of laboratory-acquired tularemia. Treatment with broad spectrum antibiotics. Am J Med. 1961 May;30:785–806. doi: 10.1016/0002-9343(61)90214-5. [DOI] [PubMed] [Google Scholar]
  14. Olsufjev N. G., Meshcheryakova I. S. Infraspecific taxonomy of tularemia agent Francisella tularensis McCoy et Chapin. J Hyg Epidemiol Microbiol Immunol. 1982;26(3):291–299. [PubMed] [Google Scholar]
  15. Pittman B., Shaw E. B., Jr, Cherry W. B. Isolation of Francisella tularensis from infected frozen human blood. J Clin Microbiol. 1977 Jun;5(6):621–624. doi: 10.1128/jcm.5.6.621-624.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ringertz O., Dahlstrand S. Culture of P. tularensis in the 1966-67 outbreaks of tularemia in Sweden. Laboratory methods and precautions against laboratory infections. Acta Pathol Microbiol Scand. 1968;72(3):464–464. [PubMed] [Google Scholar]
  17. Sawyer W. D., Dangerfield H. G., Hogge A. L., Crozier D. Antibiotic prophylaxis and therapy of airborne tularemia. Bacteriol Rev. 1966 Sep;30(3):542–550. doi: 10.1128/br.30.3.542-550.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Scharer J. M., Klein F., Lincoln R. E. Growth and metabolism of live vaccine strain of Pasteurella tularensis. Appl Microbiol. 1968 Jun;16(6):855–861. doi: 10.1128/am.16.6.855-861.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Young L. S., Bickness D. S., Archer B. G., Clinton J. M., Leavens L. J., Feeley J. C., Brachman P. S. Tularemia epidemia: Vermont, 1968. Forty-seven cases linked to contact with muskrats. N Engl J Med. 1969 Jun 5;280(23):1253–1260. doi: 10.1056/NEJM196906052802301. [DOI] [PubMed] [Google Scholar]

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