Abstract
We compared three methods for identifying clinical yeast isolates: Abbott Quantum II, API 20C, and a modified BBL Minitek system. The API 20C and modified Minitek systems agreed on the identification of 243 of 245 yeasts (99.2%). The Quantum II system correctly identified 197 (80.4%), incorrectly identified 19 (7.8%), and did not identify 29 (11.8%) of the yeasts. Most of the misidentifications with the Quantum II occurred because assimilation or biochemical results were false-positive. Sixteen different species of yeasts and 16 different Quantum II substrates contributed to the discrepancies. On retesting with the Quantum II, 31% of the discrepant strains were correctly identified, while the remaining 69% were incorrectly identified or were not identified. Erroneous biochemical and assimilation results were also noted with yeasts that were correctly identified by the Quantum II system.
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Selected References
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- Buesching W. J., Kurek K., Roberts G. D. Evaluation of the modified API 20C system for identification of clinically important yeasts. J Clin Microbiol. 1979 May;9(5):565–569. doi: 10.1128/jcm.9.5.565-569.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cooper B. H., Prowant S., Alexander B., Brunson D. H. Collaborative evaluation of the Abbott yeast identification system. J Clin Microbiol. 1984 Jun;19(6):853–856. doi: 10.1128/jcm.19.6.853-856.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kiehn T. E., Edwards F. F., Armstrong D. The prevalence of yeasts in clinical specimens from cancer patients. Am J Clin Pathol. 1980 Apr;73(4):518–521. doi: 10.1093/ajcp/73.4.518. [DOI] [PubMed] [Google Scholar]
- Land G. A., Harrison B. A., Hulme K. L., Cooper B. H., Byrd J. C. Evaluation of the new API 20C strip for yeast identification against a conventional method. J Clin Microbiol. 1979 Sep;10(3):357–364. doi: 10.1128/jcm.10.3.357-364.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Meunier-Carpentier F., Kiehn T. E., Armstrong D. Fungemia in the immunocompromised host. Changing patterns, antigenemia, high mortality. Am J Med. 1981 Sep;71(3):363–370. doi: 10.1016/0002-9343(81)90162-5. [DOI] [PubMed] [Google Scholar]
- Mickelsen P. A., McCarthy L. R., Propst M. A. Further modifications of the auxanographic method for identification of yeasts. J Clin Microbiol. 1977 Mar;5(3):297–301. doi: 10.1128/jcm.5.3.297-301.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]