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. 1985 Aug;22(2):274–278. doi: 10.1128/jcm.22.2.274-278.1985

Detection of Norwalk virus antibodies and antigen with a biotin-avidin immunoassay.

G W Gary Jr, J E Kaplan, S E Stine, L J Anderson
PMCID: PMC268374  PMID: 2993353

Abstract

Biotin-avidin immunoassays (BAIs) were developed to detect Norwalk virus antigen and to measure Norwalk virus antibody. The BAI detected Norwalk virus infections by a fourfold titer rise in antibody in sera or by antigen in stool, with a sensitivity similar to or greater than that of the radioimmunoassay (RIA), and the BAI appeared to be more sensitive than the RIA for detecting antibody in single serum specimens. The BAI antigen test detected Norwalk antigen in all stools that were positive by the RIA, and in two stools negative by the RIA. Of 106 serum pairs tested by both the RIA and BAI, 94 demonstrated seroconversion, or lack of seroconversion, in both assays; 12 gave discordant results. Titers by the BAI generally exceeded those by the RIA by two- or fourfold. The BAI had advantages over the RIA in that it had added sensitivity in the detection of Norwalk antibody, was less time consuming, did not require frequent labeling of antibody, and did not have the problems associated with the use of isotopes.

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Selected References

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  1. Adler-Storthz K., Kendall C., Kennedy R. C., Henkel R. D., Dreesman G. R. Biotin-avidin-amplified enzyme immunoassay for detection of herpes simplex virus antigen in clinical specimens. J Clin Microbiol. 1983 Dec;18(6):1329–1334. doi: 10.1128/jcm.18.6.1329-1334.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Adler J. L., Zickl R. Winter vomiting disease. J Infect Dis. 1969 Jun;119(6):668–673. doi: 10.1093/infdis/119.6.668. [DOI] [PubMed] [Google Scholar]
  3. Dolin R., Blacklow N. R., DuPont H., Formal S., Buscho R. F., Kasel J. A., Chames R. P., Hornick R., Chanock R. M. Transmission of acute infectious nonbacterial gastroenteritis to volunteers by oral administration of stool filtrates. J Infect Dis. 1971 Mar;123(3):307–312. doi: 10.1093/infdis/123.3.307. [DOI] [PubMed] [Google Scholar]
  4. Dolin R., Reichman R. C., Roessner K. D., Tralka T. S., Schooley R. T., Gary W., Morens D. Detection by immune electron microscopy of the Snow Mountain agent of acute viral gastroenteritis. J Infect Dis. 1982 Aug;146(2):184–189. doi: 10.1093/infdis/146.2.184. [DOI] [PubMed] [Google Scholar]
  5. Greenberg H. B., Valdesuso J., Kapikian A. Z., Chanock R. M., Wyatt R. G., Szmuness W., Larrick J., Kaplan J., Gilman R. H., Sack D. A. Prevalence of antibody to the Norwalk virus in various countries. Infect Immun. 1979 Oct;26(1):270–273. doi: 10.1128/iai.26.1.270-273.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Greenberg H. B., Valdesuso J., Yolken R. H., Gangarosa E., Gary W., Wyatt R. G., Konno T., Suzuki H., Chanock R. M., Kapikian A. Z. Role of Norwalk virus in outbreaks of nonbacterial gastroenteritis. J Infect Dis. 1979 May;139(5):564–568. doi: 10.1093/infdis/139.5.564. [DOI] [PubMed] [Google Scholar]
  7. Greenberg H. B., Wyatt R. G., Valdesuso J., Kalica A. R., London W. T., Chanock R. M., Kapikian A. Z. Solid-phase microtiter radioimmunoassay for detection of the Norwalk strain of acute nonbacterial, epidemic gastroenteritis virus and its antibodies. J Med Virol. 1978;2(2):97–108. doi: 10.1002/jmv.1890020204. [DOI] [PubMed] [Google Scholar]
  8. Guesdon J. L., Ternynck T., Avrameas S. The use of avidin-biotin interaction in immunoenzymatic techniques. J Histochem Cytochem. 1979 Aug;27(8):1131–1139. doi: 10.1177/27.8.90074. [DOI] [PubMed] [Google Scholar]
  9. Kapikian A. Z., Greenberg H. B., Cline W. L., Kalica A. R., Wyatt R. G., James H. D., Jr, Lloyd N. L., Chanock R. M., Ryder R. W., Kim H. W. Prevalence of antibody to the Norwalk agent by a newly developed immune adherence hemagglutination assay. J Med Virol. 1978;2(4):281–294. doi: 10.1002/jmv.1890020402. [DOI] [PubMed] [Google Scholar]
  10. Kapikian A. Z., Wyatt R. G., Dolin R., Thornhill T. S., Kalica A. R., Chanock R. M. Visualization by immune electron microscopy of a 27-nm particle associated with acute infectious nonbacterial gastroenteritis. J Virol. 1972 Nov;10(5):1075–1081. doi: 10.1128/jvi.10.5.1075-1081.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kaplan J. E., Gary G. W., Baron R. C., Singh N., Schonberger L. B., Feldman R., Greenberg H. B. Epidemiology of Norwalk gastroenteritis and the role of Norwalk virus in outbreaks of acute nonbacterial gastroenteritis. Ann Intern Med. 1982 Jun;96(6 Pt 1):756–761. doi: 10.7326/0003-4819-96-6-756. [DOI] [PubMed] [Google Scholar]
  12. Kendall C., Ionescu-Matiu I., Dreesman G. R. Utilization of the biotin/avidin system to amplify the sensitivity of the enzyme-linked immunosorbent assay (ELISA). J Immunol Methods. 1983 Feb 11;56(3):329–339. doi: 10.1016/s0022-1759(83)80022-2. [DOI] [PubMed] [Google Scholar]
  13. Subba Rao P. V., McCartney-Francis N. L., Metcalfe D. D. An avidin--biotin microELISA for rapid measurement of total and allergen-specific human IgE. J Immunol Methods. 1983 Feb 25;57(1-3):71–85. doi: 10.1016/0022-1759(83)90066-2. [DOI] [PubMed] [Google Scholar]
  14. Yolken R. H., Leister F. J., Whitcomb L. S., Santosham M. Enzyme immunoassays for the detection of bacterial antigens utilizing biotin-labeled antibody and peroxidase biotin--avidin complex. J Immunol Methods. 1983 Feb 11;56(3):319–327. doi: 10.1016/s0022-1759(83)80021-0. [DOI] [PubMed] [Google Scholar]

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