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. 1985 Sep;22(3):409–415. doi: 10.1128/jcm.22.3.409-415.1985

Synergistic hemolysis exhibited by species of staphylococci.

G A Hébert, G A Hancock
PMCID: PMC268421  PMID: 4044799

Abstract

The synergistic hemolysis reactions of 61 reference strains and 189 clinical isolates representing 17 species of staphylococci were examined on plates of Trypticase soy blood agar (BBL Microbiology Systems, Cockeysville, Md.). Some or all of the strains of Staphylococcus aureus, S. epidermidis, S. capitis, S. cohnii, S. haemolyticus, S. hyicus, S. simulans, S. warneri, and S. xylosus produced a delta-hemolysin that gave synergistic, complete hemolysis of washed human, sheep, and ox blood cells in an area of beta-lysin activity from strains of S. aureus and S. intermedius. Strains of the same nine species were positive with a commercial beta-lysin paper disk designed for presumptive identification of group B streptococci; most of these strains also gave synergistic, complete hemolysis with exotoxin from a strain of Corynebacterium pseudotuberculosis. None of the strains of S. auricularis, S. carnosus, S. caseolyticus, S. hominis, S. intermedius, S. saprophyticus, S. sciuri, or S. lentus were positive by any of these tests for synergistic hemolysis. These results indicate that a synergistic hemolysis test could prove very useful for differentiating these species; they also suggest that one role of some of these organisms in human infections could be that of a synergist. Further studies of synergism may clarify the clinical significance of these results.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aldridge K. E. Coagulase-negative staphylococci. Infect Control. 1982 Mar-Apr;3(2):161–165. doi: 10.1017/s0195941700055958. [DOI] [PubMed] [Google Scholar]
  2. Brzin B. Camp-phenomenon in staphylococci. Zentralbl Bakteriol Orig A. 1976 Aug;235(4):373–375. [PubMed] [Google Scholar]
  3. Choudhury T. K. Synergistic lysis of erythrocytes by Propionibacterium acnes. J Clin Microbiol. 1978 Aug;8(2):238–241. doi: 10.1128/jcm.8.2.238-241.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Darling C. L. Standardization and evaluation of the CAMP reaction for the prompt, presumptive identification of Streptococcus agalactiae (Lancefield group B) in clinical material. J Clin Microbiol. 1975 Feb;1(2):171–174. doi: 10.1128/jcm.1.2.171-174.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. ELEK S. D., LEVY E. Distribution of haemolysins in pathogenic and non-pathogenic staphylococci. J Pathol Bacteriol. 1950 Oct;62(4):541–554. doi: 10.1002/path.1700620405. [DOI] [PubMed] [Google Scholar]
  6. FRASER G. THE EFFECT ON ANIMAL ERYTHROCYTES OF COMBINATIONS OF DIFFUSIBLE SUBSTANCES PRODUCED BY BACTERIA. J Pathol Bacteriol. 1964 Jul;88:43–53. doi: 10.1002/path.1700880105. [DOI] [PubMed] [Google Scholar]
  7. GLADSTONE G. P., VAN HEYNINGEN W. E. Staphylococcal leucocidins. Br J Exp Pathol. 1957 Apr;38(2):123–137. [PMC free article] [PubMed] [Google Scholar]
  8. Gubash S. M. Synergistic hemolysis phenomenon shown by an alpha-toxin-producing Clostridium perfingens and streptococcal CAMP factor in presumptive streptococcal grouping. J Clin Microbiol. 1978 Nov;8(5):480–488. doi: 10.1128/jcm.8.5.480-488.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hansen M. V., Elliott L. P. New presumptive identification test for Clostridium perfringens: reverse CAMP test. J Clin Microbiol. 1980 Oct;12(4):617–619. doi: 10.1128/jcm.12.4.617-619.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kleck J. L., Donahue J. A. Production of thermostable hemolysin by cultures of Staphylococcus epidermidis. J Infect Dis. 1968 Jun;118(3):317–323. doi: 10.1093/infdis/118.3.317. [DOI] [PubMed] [Google Scholar]
  11. Kloos W. E., Schleifer K. H. Simplified scheme for routine identification of human Staphylococcus species. J Clin Microbiol. 1975 Jan;1(1):82–88. doi: 10.1128/jcm.1.1.82-88.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kocur M., Precechtel F., Martinec T. Haemolysins in coagulase-negative staphylococci. J Pathol Bacteriol. 1966 Oct;92(2):331–336. doi: 10.1002/path.1700920210. [DOI] [PubMed] [Google Scholar]
  13. MARKS J. Recognition of pathogenic staphylococci: with notes on non-specific staphylococcal haemolysin. J Pathol Bacteriol. 1952 Jan;64(1):175–186. doi: 10.1002/path.1700640118. [DOI] [PubMed] [Google Scholar]
  14. MARKS J., VAUGHAN A. C. T. Staphylococcal delta-haemolysin. J Pathol Bacteriol. 1950 Oct;62(4):597–615. doi: 10.1002/path.1700620411. [DOI] [PubMed] [Google Scholar]
  15. MURPHY J. M., STUART O. M., REED F. I. An evaluation of the CAMP test for the identification of Streptococcus agalactiae in routine mastitis testing. Cornell Vet. 1952 Jan;42(1):133–147. [PubMed] [Google Scholar]
  16. Males B. M., Rogers W. A., Jr, Parisi J. T. Virulence factors of biotypes of Staphylococcus epidermidis from clinical sources. J Clin Microbiol. 1975 Mar;1(3):256–261. doi: 10.1128/jcm.1.3.256-261.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Skalka B., Smola J., Pillich J. A simple method of detecting staphylococcal hemolysins. Zentralbl Bakteriol Orig A. 1979;245(3):283–286. [PubMed] [Google Scholar]
  18. Wilkinson H. W. CAMP-disk test for presumptive identification of group B streptococci. J Clin Microbiol. 1977 Jul;6(1):42–45. doi: 10.1128/jcm.6.1.42-45.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. ZEMELMAN R., LONGERI L. CHARACTERIZATION OF STAPHYLOCOCCI ISOLATED FROM RAW MILK. Appl Microbiol. 1965 Mar;13:167–170. doi: 10.1128/am.13.2.167-170.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]

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