Abstract
The identity of each of 239 yeasts, encompassing 9 genera and 30 species, was determined with the Quantum II and API 20C identification systems. With API 20C results accepted as being correct, Quantum II proved to be 92% accurate in identification of common isolates, e.g., Candida albicans and Torulopsis glabrata, but only 73% effective with less frequently encountered yeasts, e.g., Trichosporon beigelii and Rhodotorula glutinis. Overall, Quantum II was 86% as accurate as API 20C for the yeast isolates tested. In addition, physical problems were encountered in inoculation of Quantum II cartridges and in the automated reading of some biochemical tests. We conclude that Quantum II is not yet suitable for routine identification of clinical yeast isolates.
Full text
PDF


Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ahearn D. G., Lawrence J. B. Disseminated candidiasis caused by a sucrose-negative variant of Candida tropicalis. J Clin Microbiol. 1984 Aug;20(2):187–190. doi: 10.1128/jcm.20.2.187-190.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ahearn D. G., McGlohn M. S. In vitro susceptibilities of sucrose-negative Candida tropicalis, Candida lusitaniae, and Candida norvegensis to amphotericin B, 5-fluorocytosine, miconazole, and ketoconazole. J Clin Microbiol. 1984 Mar;19(3):412–416. doi: 10.1128/jcm.19.3.412-416.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Baker J. G., Salkin I. F., Pincus D. H., Bodensteiner D. C., D'Amato R. F. Pathogenicity of Candida paratropicalis. Arch Pathol Lab Med. 1983 Nov;107(11):577–579. [PubMed] [Google Scholar]
- Bodey G. P. Microbiologic aspects in patients with leukemia. Hum Pathol. 1974 Nov;5(6):687–698. doi: 10.1016/s0046-8177(74)80039-0. [DOI] [PubMed] [Google Scholar]
- Buesching W. J., Kurek K., Roberts G. D. Evaluation of the modified API 20C system for identification of clinically important yeasts. J Clin Microbiol. 1979 May;9(5):565–569. doi: 10.1128/jcm.9.5.565-569.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cho S. Y., Choi H. Y. Opportunistic fungal infection among cancer patients. A ten-year autopsy study. Am J Clin Pathol. 1979 Oct;72(4):617–621. doi: 10.1093/ajcp/72.4.617. [DOI] [PubMed] [Google Scholar]
- Cooper B. H., Johnson J. B., Thaxton E. S. Clinical evaluation of the Uni-Yeast-Tek system for rapid presumptive identification of medically important yeasts. J Clin Microbiol. 1978 Apr;7(4):349–355. doi: 10.1128/jcm.7.4.349-355.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cooper B. H., Prowant S., Alexander B., Brunson D. H. Collaborative evaluation of the Abbott yeast identification system. J Clin Microbiol. 1984 Jun;19(6):853–856. doi: 10.1128/jcm.19.6.853-856.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dick J. D., Rosengard B. R., Merz W. G., Stuart R. K., Hutchins G. M., Saral R. Fatal disseminated candidiasis due to amphotericin-B-resistant Candida guilliermondii. Ann Intern Med. 1985 Jan;102(1):67–68. doi: 10.7326/0003-4819-102-1-67. [DOI] [PubMed] [Google Scholar]
- Guinet R., Chanas J., Goullier A., Bonnefoy G., Ambroise-Thomas P. Fatal septicemia due to amphotericin B-resistant Candida lusitaniae. J Clin Microbiol. 1983 Aug;18(2):443–444. doi: 10.1128/jcm.18.2.443-444.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hasyn J. J., Buckley H. R. Evaluation of the AutoMicrobic system for identification of yeasts. J Clin Microbiol. 1982 Nov;16(5):901–904. doi: 10.1128/jcm.16.5.901-904.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Land G. A., Harrison B. A., Hulme K. L., Cooper B. H., Byrd J. C. Evaluation of the new API 20C strip for yeast identification against a conventional method. J Clin Microbiol. 1979 Sep;10(3):357–364. doi: 10.1128/jcm.10.3.357-364.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Land G., Stotler R., Land K., Staneck J. Update and evaluation of the AutoMicrobic yeast identification system. J Clin Microbiol. 1984 Oct;20(4):649–652. doi: 10.1128/jcm.20.4.649-652.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Libertin C. R., Wilson W. R., Roberts G. D. Candida lusitaniae--an opportunistic pathogen. Diagn Microbiol Infect Dis. 1985 Jan;3(1):69–71. doi: 10.1016/0732-8893(85)90069-0. [DOI] [PubMed] [Google Scholar]
- Merz W. G. Candida lusitaniae: frequency of recovery, colonization, infection, and amphotericin B resistance. J Clin Microbiol. 1984 Dec;20(6):1194–1195. doi: 10.1128/jcm.20.6.1194-1195.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Meunier-Carpentier F., Kiehn T. E., Armstrong D. Fungemia in the immunocompromised host. Changing patterns, antigenemia, high mortality. Am J Med. 1981 Sep;71(3):363–370. doi: 10.1016/0002-9343(81)90162-5. [DOI] [PubMed] [Google Scholar]
- Wingard J. R., Dick J. D., Merz W. G., Sandford G. R., Saral R., Burns W. H. Pathogenicity of Candida tropicalis and Candida albicans after gastrointestinal inoculation in mice. Infect Immun. 1980 Aug;29(2):808–813. doi: 10.1128/iai.29.2.808-813.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wingard J. R., Merz W. G., Saral R. Candida tropicalis: a major pathogen in immunocompromised patients. Ann Intern Med. 1979 Oct;91(4):539–543. doi: 10.7326/0003-4819-91-4-539. [DOI] [PubMed] [Google Scholar]
- Wright S. H., Czaja A. J., Katz R. S., Soloway R. D. Systemic mycosis complicating high dose corticosteroid treatment of chronic active liver disease. Am J Gastroenterol. 1980 Nov;74(5):428–432. [PubMed] [Google Scholar]