Abstract
The comparative susceptibilities for rhinovirus isolation were determined for strain MRC-5 human embryonic lung fibroblast and human fetal tonsil cells. Samples from nasopharyngeal and throat swab clinical specimens were inoculated onto duplicate monolayers of each cell type. Of 105 rhinovirus-positive specimens, 78 (74%) were positive in MRC-5 cells, and 102 (97%) were positive in fetal tonsil cells (P less than 0.001). For 75 specimens positive in both, the mean time to cytopathic effect development with standard deviation was 1 +/- 2 days shorter in fetal tonsil cells (P less than 0.01). The use of a second virus culture increased rhinovirus isolations by 10% compared with a single culture.
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Selected References
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- Cooney M. K., Kenny G. E. Demonstration of dual rhinovirus infection in humans by isolation of different serotypes in human heteroploid (HeLa) and human diploid fibroblast cell cultures. J Clin Microbiol. 1977 Feb;5(2):202–207. doi: 10.1128/jcm.5.2.202-207.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fox J. P., Cooney M. K., Hall C. E. The Seattle virus watch. V. Epidemiologic observations of rhinovirus infections, 1965-1969, in families with young children. Am J Epidemiol. 1975 Feb;101(2):122–143. doi: 10.1093/oxfordjournals.aje.a112078. [DOI] [PubMed] [Google Scholar]
- Gregory W. W., Menegus M. A. Practical protocol for cytomegalovirus isolation: use of MRC-5 cell monolayers incubated for 2 weeks. J Clin Microbiol. 1983 Apr;17(4):605–609. doi: 10.1128/jcm.17.4.605-609.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hayden F. G., Gwaltney J. M., Jr Anti-interferon antibody increases rhinovirus isolation rates from nasal wash specimens containing interferon-alpha 2. Antiviral Res. 1983 Mar;3(1):67–71. doi: 10.1016/0166-3542(83)90016-5. [DOI] [PubMed] [Google Scholar]