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. 1985 Oct;22(4):527–530. doi: 10.1128/jcm.22.4.527-530.1985

Long-lasting cell-mediated immunity induced by a live Francisella tularensis vaccine.

A Tärnvik, M L Löfgren, S Löfgren, G Sandström, H Wolf-Watz
PMCID: PMC268460  PMID: 3878364

Abstract

The lymphocyte stimulation test was used to estimate specific cell-mediated immunity after vaccination with the live vaccine strain of Francisella tularensis. Nonvaccinated individuals and individuals vaccinated 1, 5 to 6, 7 to 8, and 9 years previously were tested. Lymphocytes from most vaccinees responded to an antigen preparation of the vaccine strain, and those vaccinated 9 years before testing responded to a similar extent as did vaccines in the other groups. A new technique was developed to study the presence of T lymphocytes among the stimulated cells. Stimulated cells were allowed to incorporate [14C]thymidine and were then fractionated into T and non-T lymphocytes. Most of the incorporation appeared in the fraction containing T lymphocytes. The data indicate that cell-mediated immunity endures for at least 9 years after vaccination with the live F. tularensis vaccine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burke D. S. Immunization against tularemia: analysis of the effectiveness of live Francisella tularensis vaccine in prevention of laboratory-acquired tularemia. J Infect Dis. 1977 Jan;135(1):55–60. doi: 10.1093/infdis/135.1.55. [DOI] [PubMed] [Google Scholar]
  2. Carlsson H. E., Lindberg A. A., Lindberg G., Hederstedt B., Karlsson K. A., Agell B. O. Enzyme-linked immunosorbent assay for immunological diagnosis of human tularemia. J Clin Microbiol. 1979 Nov;10(5):615–621. doi: 10.1128/jcm.10.5.615-621.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Claflin J. L., Larson C. L. Infection-immunity in tularemia: specificity of cellular immunity. Infect Immun. 1972 Mar;5(3):311–318. doi: 10.1128/iai.5.3.311-318.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. EIGELSBACH H. T., DOWNS C. M. Prophylactic effectiveness of live and killed tularemia vaccines. I. Production of vaccine and evaluation in the white mouse and guinea pig. J Immunol. 1961 Oct;87:415–425. [PubMed] [Google Scholar]
  5. Hartzman R. J., Bach M. L., Bach F. H., Thurman G. B., Sell K. W. Precipitation of radioactively labeled samples: a semi-automatic multiple-sample processor. Cell Immunol. 1972 Jun;4(2):182–186. doi: 10.1016/0008-8749(72)90018-4. [DOI] [PubMed] [Google Scholar]
  6. Jensen B., Thorarinsdottir T., Møller S., Bentzon M. W. Antigen specific in vitro activity of lymphocyte subpopulations from Mantoux positive and negative subjects assessed by lymphocyte proliferation assay. J Immunol Methods. 1984 Jan 20;66(1):133–148. doi: 10.1016/0022-1759(84)90256-4. [DOI] [PubMed] [Google Scholar]
  7. Jondal M. Surface markers on human B and T lymphocytes. IV. Distribution of surface markers on resting and blast-transformed lymphocytes. Scand J Immunol. 1974;3(6):739–747. doi: 10.1111/j.1365-3083.1974.tb01309.x. [DOI] [PubMed] [Google Scholar]
  8. Koskela P., Herva E. Cell-mediated and humoral immunity induced by a live Francisella tularensis vaccine. Infect Immun. 1982 Jun;36(3):983–989. doi: 10.1128/iai.36.3.983-989.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kostiala A. A., McGregor D. D., Logie P. S. Tularaemia in the rat. I. The cellular basis on host resistance to infection. Immunology. 1975 May;28(5):855–869. [PMC free article] [PubMed] [Google Scholar]
  10. North R. J. Nature of "memory" in T-cell-mediated antibacterial immunity: anamnestic production of mediator T cells. Infect Immun. 1975 Oct;12(4):754–760. doi: 10.1128/iai.12.4.754-760.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nutter J. E., Myrvik Q. N. In vitro interactions between rabbit alveolar macrophages and Pasteurella tularensis. J Bacteriol. 1966 Sep;92(3):645–651. doi: 10.1128/jb.92.3.645-651.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. OVERHOLT E. L., TIGERTT W. D., KADULL P. J., WARD M. K., CHARKES N. D., RENE R. M., SALZMAN T. E., STEPHENS M. An analysis of forty-two cases of laboratory-acquired tularemia. Treatment with broad spectrum antibiotics. Am J Med. 1961 May;30:785–806. doi: 10.1016/0002-9343(61)90214-5. [DOI] [PubMed] [Google Scholar]
  13. Sandström G., Tärnvik A., Wolf-Watz H., Löfgren S. Antigen from Francisella tularensis: nonidentity between determinants participating in cell-mediated and humoral reactions. Infect Immun. 1984 Jul;45(1):101–106. doi: 10.1128/iai.45.1.101-106.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Tärnvik A., Holm S. E. Stimulation of subpopulations of human lymphocytes by a vaccine strain of Francisella tularensis. Infect Immun. 1978 Jun;20(3):698–704. doi: 10.1128/iai.20.3.698-704.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tärnvik A., Löfgren S. Stimulation of human lymphocytes by a vaccine strain of Francisella tularensis. Infect Immun. 1975 Nov;12(5):951–957. doi: 10.1128/iai.12.5.951-957.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tärnvik A., Sandström G., Löfgren S. Time of lymphocyte response after onset of tularemia and after tularemia vaccination. J Clin Microbiol. 1979 Dec;10(6):854–860. doi: 10.1128/jcm.10.6.854-860.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. VAN METRE T. E., Jr, KADULL P. J. Laboratory-acquired tularemia in vaccinated individuals: a report of 62 cases. Ann Intern Med. 1959 Mar;50(3):621–632. doi: 10.7326/0003-4819-50-3-621. [DOI] [PubMed] [Google Scholar]

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