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. 1985 Oct;22(4):576–581. doi: 10.1128/jcm.22.4.576-581.1985

Enterotoxigenic Escherichia coli in a population of infants with diarrhea in Chile.

M E Agüero, L Reyes, V Prado, I Orskov, F Orskov, F C Cabello
PMCID: PMC268470  PMID: 3908470

Abstract

The incidence of enterotoxigenic Escherichia coli (ETEC) was investigated in 95 E. coli strains isolated from 48 infants with diarrhea in Santiago, Chile. By using standard biological assays and DNA-DNA hybridization procedures, ETEC was found in 31.2% of the cases: 14 strains produced heat-stable enterotoxin (ST) only, three strains produced heat-labile enterotoxin (LT) and ST, and two strains produced LT only. DNA probes detected all enterotoxin producers except one ST-producing strain. The ST strains hybridized with one or both of the human ST probes (ST Ib and ST A2). Two of the LT-ST strains hybridized with the ST Ia and ST Ib probes, and the third strain did not hybridize with any of the ST probes. Only the ST group expressed multiple resistance (85.7%) and colonization factor antigen I (CFA I) (92.8%); CFA II was found in two of three LT-ST strains. The O153:H45 serotype was found in 10 of 14 ST strains, and O6:K15:H16 was found in one LT strain and in two LT-ST strains. These findings suggest that ETEC, especially strains that produce ST, may be an important cause of diarrhea among Chilean infants.

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Selected References

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  1. Bauer A. W., Kirby W. M., Sherris J. C., Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966 Apr;45(4):493–496. [PubMed] [Google Scholar]
  2. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Black R. E., Brown K. H., Becker S., Alim A. R., Huq I. Longitudinal studies of infectious diseases and physical growth of children in rural Bangladesh. II. Incidence of diarrhea and association with known pathogens. Am J Epidemiol. 1982 Mar;115(3):315–324. doi: 10.1093/oxfordjournals.aje.a113308. [DOI] [PubMed] [Google Scholar]
  4. Black R. E., Merson M. H., Rahman A. S., Yunus M., Alim A. R., Huq I., Yolken R. H., Curlin G. T. A two-year study of bacterial, viral, and parasitic agents associated with diarrhea in rural Bangladesh. J Infect Dis. 1980 Nov;142(5):660–664. doi: 10.1093/infdis/142.5.660. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cabello F., Agüero M. E., Fernández M. E. Epidemia de fiebre tifoidea en Chile: aspectos ecológicos y microbiológicos. Rev Med Chil. 1984 Aug;112(8):826–828. [PubMed] [Google Scholar]
  6. Dallas W. S., Gill D. M., Falkow S. Cistrons encoding Escherichia coli heat-labile toxin. J Bacteriol. 1979 Sep;139(3):850–858. doi: 10.1128/jb.139.3.850-858.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dean A. G., Ching Y. C., Williams R. G., Harden L. B. Test for Escherichia coli enterotoxin using infant mice: application in a study of diarrhea in children in Honolulu. J Infect Dis. 1972 Apr;125(4):407–411. doi: 10.1093/infdis/125.4.407. [DOI] [PubMed] [Google Scholar]
  8. Deneke C. F., Thorne G. M., Gorbach S. L. Attachment pili from enterotoxigenic Escherichia coli pathogenic for humans. Infect Immun. 1979 Oct;26(1):362–368. doi: 10.1128/iai.26.1.362-368.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Donta S. T., Moon H. W., Whipp S. C. Detection of heat-labile Escherichia coli enterotoxin with the use of adrenal cells in tissue culture. Science. 1974 Jan 25;183(4122):334–336. doi: 10.1126/science.183.4122.334. [DOI] [PubMed] [Google Scholar]
  10. Donta S. T., Wallace R. B., Whipp S. C., Olarte J. Enterotoxigenic Escherichia coli and diarrheal disease in Mexican children. J Infect Dis. 1977 Mar;135(3):482–485. doi: 10.1093/infdis/135.3.482. [DOI] [PubMed] [Google Scholar]
  11. Echeverria P., Blacklow N. R., Sanford L. B., Cukor G. G. Travelers' diarrhea among American Peace Corps volunteers in rural Thailand. J Infect Dis. 1981 Jun;143(6):767–771. doi: 10.1093/infdis/143.6.767. [DOI] [PubMed] [Google Scholar]
  12. Echeverria P., Ho M. T., Blacklow N. R., Quinnan G., Portnoy B., Olson J. G., Conklin R., DuPont H. L., Cross J. H. Relative importance of viruses and bacteria in the etiology of pediatric diarrhea in Taiwan. J Infect Dis. 1977 Sep;136(3):383–390. doi: 10.1093/infdis/136.3.383. [DOI] [PubMed] [Google Scholar]
  13. Echeverria P., Verhaert L., Ulyangco C. V., Komalarini S., Ho M. T., Orskov F., Orskov I. Antimicrobial resistance and enterotoxin production among isolates of Escherichia coli in the Far East. Lancet. 1978 Sep 16;2(8090):589–592. doi: 10.1016/s0140-6736(78)92820-9. [DOI] [PubMed] [Google Scholar]
  14. Evans D. G., Evans D. J., Jr New surface-associated heat-labile colonization factor antigen (CFA/II) produced by enterotoxigenic Escherichia coli of serogroups O6 and O8. Infect Immun. 1978 Aug;21(2):638–647. doi: 10.1128/iai.21.2.638-647.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Evans D. G., Evans D. J., Jr, Tjoa W. Hemagglutination of human group A erythrocytes by enterotoxigenic Escherichia coli isolated from adults with diarrhea: correlation with colonization factor. Infect Immun. 1977 Nov;18(2):330–337. doi: 10.1128/iai.18.2.330-337.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Evans D. G., Olarte J., DuPont H. L., Evans D. J., Jr, Galindo E., Portnoy B. L., Conklin R. H. Enteropathogens associated with pediatric diarrhea in Mexico City. J Pediatr. 1977 Jul;91(1):65–68. doi: 10.1016/s0022-3476(77)80446-0. [DOI] [PubMed] [Google Scholar]
  17. Evans D. J., Jr, Evans D. G., DuPont H. L. Hemagglutination patterns of enterotoxigenic and enteropathogenic Escherichia coli determined with human, bovine, chicken, and guinea pig erythrocytes in the presence and absence of mannose. Infect Immun. 1979 Feb;23(2):336–346. doi: 10.1128/iai.23.2.336-346.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Figueroa Gronemeyer T. M. Estudio de prevalencia de serotipos enteropatógenos de Escherichia coli en deposiciones de niños que habitan una población suburbana de Santiago. Rev Chil Pediatr. 1980 May-Jun;51(3):225–227. [PubMed] [Google Scholar]
  19. Georges M. C., Wachsmuth I. K., Birkness K. A., Moseley S. L., Georges A. J. Genetic probes for enterotoxigenic Escherichia coli isolated from childhood diarrhea in the Central African Republic. J Clin Microbiol. 1983 Jul;18(1):199–202. doi: 10.1128/jcm.18.1.199-202.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Giannella R. A. Suckling mouse model for detection of heat-stable Escherichia coli enterotoxin: characteristics of the model. Infect Immun. 1976 Jul;14(1):95–99. doi: 10.1128/iai.14.1.95-99.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Gorbach S. L., Kean B. H., Evans D. G., Evans D. J., Jr, Bessudo D. Travelers' diarrhea and toxigenic Escherichia coli. N Engl J Med. 1975 May 1;292(18):933–936. doi: 10.1056/NEJM197505012921801. [DOI] [PubMed] [Google Scholar]
  22. Guerrant R. L., Kirchhoff L. V., Shields D. S., Nations M. K., Leslie J., de Sousa M. A., Araujo J. G., Correia L. L., Sauer K. T., McClelland K. E. Prospective study of diarrheal illnesses in northeastern Brazil: patterns of disease, nutritional impact, etiologies, and risk factors. J Infect Dis. 1983 Dec;148(6):986–997. doi: 10.1093/infdis/148.6.986. [DOI] [PubMed] [Google Scholar]
  23. Guerrant R. L., Moore R. A., Kirschenfeld P. M., Sande M. A. Role of toxigenic and invasive bacteria in acute diarrhea of childhood. N Engl J Med. 1975 Sep 18;293(12):567–572. doi: 10.1056/NEJM197509182931201. [DOI] [PubMed] [Google Scholar]
  24. Guerrant R. L., Rouse J. D., Hughes J. M., Rowe B. Turista among members of the Yale Glee Club in Latin America. Am J Trop Med Hyg. 1980 Sep;29(5):895–900. doi: 10.4269/ajtmh.1980.29.895. [DOI] [PubMed] [Google Scholar]
  25. Lathe R., Hirth P., DeWilde M., Harford N., Lecocq J. P. Cell-free synthesis of enterotoxin of E. coli from a cloned gene. Nature. 1980 Apr 3;284(5755):473–474. doi: 10.1038/284473a0. [DOI] [PubMed] [Google Scholar]
  26. Leksomboon U., Echeverria P., Suvongse C., Duangmani C. Viruses and bacteria in pediatric diarrhea in Thailand: a study of multiple antibiotic-resistant enteric pathogens. Am J Trop Med Hyg. 1981 Nov;30(6):1281–1290. doi: 10.4269/ajtmh.1981.30.1281. [DOI] [PubMed] [Google Scholar]
  27. Macrina F. L., Kopecko D. J., Jones K. R., Ayers D. J., McCowen S. M. A multiple plasmid-containing Escherichia coli strain: convenient source of size reference plasmid molecules. Plasmid. 1978 Jun;1(3):417–420. doi: 10.1016/0147-619x(78)90056-2. [DOI] [PubMed] [Google Scholar]
  28. Maniatis T., Jeffrey A., Kleid D. G. Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184–1188. doi: 10.1073/pnas.72.3.1184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Merson M. H., Orskov F., Orskov I., Sack R. B., Huq I., Koster F. T. Relationship between enterotoxin production and serotype in enterotoxigenic Escherichia coli. Infect Immun. 1979 Feb;23(2):325–329. doi: 10.1128/iai.23.2.325-329.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Moseley S. L., Echeverria P., Seriwatana J., Tirapat C., Chaicumpa W., Sakuldaipeara T., Falkow S. Identification of enterotoxigenic Escherichia coli by colony hybridization using three enterotoxin gene probes. J Infect Dis. 1982 Jun;145(6):863–869. doi: 10.1093/infdis/145.6.863. [DOI] [PubMed] [Google Scholar]
  31. Moseley S. L., Hardy J. W., Hug M. I., Echeverria P., Falkow S. Isolation and nucleotide sequence determination of a gene encoding a heat-stable enterotoxin of Escherichia coli. Infect Immun. 1983 Mar;39(3):1167–1174. doi: 10.1128/iai.39.3.1167-1174.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Moseley S. L., Huq I., Alim A. R., So M., Samadpour-Motalebi M., Falkow S. Detection of enterotoxigenic Escherichia coli by DNA colony hybridization. J Infect Dis. 1980 Dec;142(6):892–898. doi: 10.1093/infdis/142.6.892. [DOI] [PubMed] [Google Scholar]
  33. Orskov I., Orskov F. Special O:K:H serotypes among enterotoxigenic E. coli strains from diarrhea in adults and children. Occurrence of the CF (colonization factor) antigen and of hemagglutinating abilities. Med Microbiol Immunol. 1977 Jul 18;163(2):99–110. doi: 10.1007/BF02121825. [DOI] [PubMed] [Google Scholar]
  34. Pickering L. K., Evans D. J., Jr, Muñoz O., DuPont H. L., Coello-Ramírez P., Vollet J. J., Conklin R. H., Olarte J., Kohl S. Prospective study of enteropathogens in children with diarrhea in Houston and Mexico. J Pediatr. 1978 Sep;93(3):383–388. doi: 10.1016/s0022-3476(78)81142-1. [DOI] [PubMed] [Google Scholar]
  35. Prado V., Braun S., Bosch P., Bercovich M., Reyes L., Sawada M. Análisis de Escherichia coli enteropatógeno clásico (E.C.E.P.) como causa endémica de diarrea aguda en niños chilenos. Rev Chil Pediatr. 1984 May-Jun;55(3):171–175. [PubMed] [Google Scholar]
  36. Prado V., Siri M. T., Aranda M., Marambio E., Cohen J. Incidencia de E. coli enterotoxigénico en lactantes con diarrea aguda. Utilidad de células LLC para la detección de toxina termolabil (LT). Rev Med Chil. 1983 Dec;111(12):1221–1226. [PubMed] [Google Scholar]
  37. Reis M. H., Guth B. E., Gomes T. A., Murahovschi J., Trabulsi L. R. Frequency of Escherichia coli strains producing heat-labile toxin or heat-stable toxin or both in children with and without diarrhea in São Paulo. J Clin Microbiol. 1982 Jun;15(6):1062–1064. doi: 10.1128/jcm.15.6.1062-1064.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Reis M. H., Matos D. P., de Castro A. F., Toledo M. R., Trabulsi L. R. Relationship among enterotoxigenic phenotypes, serotypes, and sources of strains in enterotoxigenic Escherichia coli. Infect Immun. 1980 Apr;28(1):24–27. doi: 10.1128/iai.28.1.24-27.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Sack D. A., Merson M. H., Wells J. G., Sack R. B., Morris G. K. Diarrhoea associated with heat-stable enterotoxin-producing strains of Escherichia coli. Lancet. 1975 Aug 9;2(7928):239–241. doi: 10.1016/s0140-6736(75)90958-7. [DOI] [PubMed] [Google Scholar]
  40. Sack D. A., Sack R. B. Test for enterotoxigenic Escherichia coli using Y-1 adrenal cells in miniculture. Infect Immun. 1975 Feb;11(2):334–336. doi: 10.1128/iai.11.2.334-336.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Scotland S. M., Gross R. J., Cheasty T., Rowe B. The occurrence of plasmids carrying genes for both enterotoxin production and drug resistance in Escherichia coli of human origin. J Hyg (Lond) 1979 Dec;83(3):531–538. doi: 10.1017/s0022172400026383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. Seriwatana J., Echeverria P., Escamilla J., Glass R., Huq I., Rockhill R., Stoll B. J. Identification of enterotoxigenic Escherichia coli in patients with diarrhea in Asia with three enterotoxin gene probes. Infect Immun. 1983 Oct;42(1):152–155. doi: 10.1128/iai.42.1.152-155.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  44. Spencer E., Avendaño F., Araya M. Characteristics and analysis of electropherotypes of human rotavirus isolated in Chile. J Infect Dis. 1983 Jul;148(1):41–48. doi: 10.1093/infdis/148.1.41. [DOI] [PubMed] [Google Scholar]
  45. Thorne G. M., Deneke C. F., Gorbach S. L. Hemagglutination and adhesiveness of toxigenic Escherichia coli isolated from humans. Infect Immun. 1979 Mar;23(3):690–699. doi: 10.1128/iai.23.3.690-699.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  46. Wachsmuth K., DeBoy J., Birkness K., Sack D., Wells J. Genetic transfer of antimicrobial resistance and enterotoxigenicity among Escherichia coli strains. Antimicrob Agents Chemother. 1983 Feb;23(2):278–283. doi: 10.1128/aac.23.2.278. [DOI] [PMC free article] [PubMed] [Google Scholar]
  47. Wadström T., Aust-Kettis A., Habte D., Holmgren J., Meeuwisse G., Möllby R., Söderlind O. Enterotoxin-producing bacteria and parasites in stools of Ethiopian children with diarrhoeal disease. Arch Dis Child. 1976 Nov;51(11):865–870. doi: 10.1136/adc.51.11.865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  48. Zacarías J., Spencer E., Prado V., Herskovic P., Cohen J., Tagle S., Cienfuegos G., Araya M. Recuperación fecal de rotavirus y otros enteropatógenos en lactantes menores de un año con diarrea aguda. Rev Chil Pediatr. 1982 Mar-Apr;53(2):111–116. [PubMed] [Google Scholar]

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