Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1985 Oct;22(4):641–644. doi: 10.1128/jcm.22.4.641-644.1985

Detection of herpes simplex virus type 2-specific antibody with glycoprotein G.

F K Lee, R M Coleman, L Pereira, P D Bailey, M Tatsuno, A J Nahmias
PMCID: PMC268483  PMID: 3001136

Abstract

A recently described herpes simplex virus (HSV) type 2 (HSV-2)-specific glycoprotein (gG-2) was purified on an immunoaffinity column prepared with monoclonal antibody. This purified antigen was used in an immunodot enzymatic assay on nitrocellulose paper for the detection of HSV-2 antibodies in human serum. The test was very sensitive in that HSV-2 antibodies were detected in the convalescent sera of 132 of 134 patients with recurrent genital infections in which HSV-2 had been isolated earlier. Antibodies to gG-2 were detected in 17% of sera obtained within 10 days after the onset of a primary HSV infection and in 95% of sera obtained more than 10 days after onset. The specificity of the immunodot assay was demonstrated by testing sera from 245 HSV-seronegative adults, 344 children, 29 nuns, and 13 patients with primary genital HSV-1 infections. None of these 631 sera was reactive with the gG-2 antigen. When compared with a microneutralization test, the immunodot assay was found to be more specific in detecting HSV-2 antibodies. Reproducibility of the gG-2 assay, obtained by retesting 391 sera, was 95%. Thus, this assay has the sensitivity, specificity, and reproducibility necessary for the measurement of HSV-2 antibodies in seroepidemiological studies.

Full text

PDF
641

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arvin A. M., Koropchak C. M., Yeager A. S., Pereira L. Detection of type-specific antibody to herpes simplex virus type 1 by radioimmunoassay with herpes simplex virus type 1 glycoprotein C purified with monoclonal antibody. Infect Immun. 1983 Apr;40(1):184–189. doi: 10.1128/iai.40.1.184-189.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Buchman T. G., Simpson T., Nosal C., Roizman B., Nahmias A. J. The structure of herpes simplex virus DNA and its application to molecular epidemiology. Ann N Y Acad Sci. 1980;354:279–290. doi: 10.1111/j.1749-6632.1980.tb27972.x. [DOI] [PubMed] [Google Scholar]
  3. Coleman R. M., Pereira L., Bailey P. D., Dondero D., Wickliffe C., Nahmias A. J. Determination of herpes simplex virus type-specific antibodies by enzyme-linked immunosorbent assay. J Clin Microbiol. 1983 Aug;18(2):287–291. doi: 10.1128/jcm.18.2.287-291.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Marsden H. S., Stow N. D., Preston V. G., Timbury M. C., Wilkie N. M. Physical mapping of herpes simplex virus-induced polypeptides. J Virol. 1978 Nov;28(2):624–642. doi: 10.1128/jvi.28.2.624-642.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Nahmias A. J., Dowdle W. R. Antigenic and biologic differences in herpesvirus hominis. Prog Med Virol. 1968;10:110–159. [PubMed] [Google Scholar]
  6. Nahmias A. J., Dowdle W. R., Kramer J. H., Luce C. F., Mansour S. C. Antibodies to Herpesvirus hominis types 1 and 2 in the rabbit. J Immunol. 1969 Apr;102(4):956–962. [PubMed] [Google Scholar]
  7. Nahmias A. J., Josey W. E., Naib Z. M., Luce C. F., Duffey A. Antibodies to Herpesvirus hominis types 1 and 2 in humans. I. Patients with genital herpetic infections. Am J Epidemiol. 1970 Jun;91(6):539–546. doi: 10.1093/oxfordjournals.aje.a121165. [DOI] [PubMed] [Google Scholar]
  8. Patterson W. R., Rawls W. E., Smith K. O. Differentiation of serum antibodies to herpesvirus types 1 and 2 by radioimmunoassay. Proc Soc Exp Biol Med. 1978 Feb;157(2):273–277. doi: 10.3181/00379727-157-40036. [DOI] [PubMed] [Google Scholar]
  9. Pereira L., Dondero D. V., Gallo D., Devlin V., Woodie J. D. Serological analysis of herpes simplex virus types 1 and 2 with monoclonal antibodies. Infect Immun. 1982 Jan;35(1):363–367. doi: 10.1128/iai.35.1.363-367.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Roizman B., Norrild B., Chan C., Pereira L. Identification and preliminary mapping with monoclonal antibodies of a herpes simplex virus 2 glycoprotein lacking a known type 1 counterpart. Virology. 1984 Feb;133(1):242–247. doi: 10.1016/0042-6822(84)90447-1. [DOI] [PubMed] [Google Scholar]
  11. SCHNEWEIS K. E. [Serological studies on the type differentiation of Herpesvirus hominis]. Z Immun exp ther. 1962 Sep;124:24–48. [PubMed] [Google Scholar]
  12. Schneweis K. E., Nahmias A. J. Antigens of Herpes simplex virus type 1 and 2-immunodiffusion and inhibition passive hemagglutination studies. Z Immunitatsforsch Exp Klin Immunol. 1971 Jun;141(5):471–487. [PubMed] [Google Scholar]
  13. Stavraky K. M., Rawls W. E., Chiavetta J., Donner A. P., Wanklin J. M. Sexual and socioeconomic factors affecting the risk of past infections with herpes simplex virus type 2. Am J Epidemiol. 1983 Jul;118(1):109–121. doi: 10.1093/oxfordjournals.aje.a113612. [DOI] [PubMed] [Google Scholar]
  14. Vonka V., Kanka J., Hirsch I., Závadová H., Krcmár M., Suchánková A., Rezácová D., Broucek J., Press M., Domorázková E. Prospective study on the relationship between cervical neoplasia and herpes simplex type-2 virus. II. Herpes simplex type-2 antibody presence in sera taken at enrollment. Int J Cancer. 1984 Jan 15;33(1):61–66. doi: 10.1002/ijc.2910330111. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES