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. 1985 Oct;22(4):677–679. doi: 10.1128/jcm.22.4.677-679.1985

Enzyme release of antigen from Streptococcus faecalis and Listeria monocytogenes cross-reactive with Lancefield group G typing reagents.

R L Hopfer, R Pinzon, M Wenglar, K V Rolston
PMCID: PMC268495  PMID: 3935666

Abstract

Enzyme extraction methods are commonly used to release antigen for Lancefield serogrouping of streptococci. Recently, up to 47% of enterococci have been found to possess antigens cross-reactive with both the group D and the group G grouping reagents. We investigated whether the organisms had recently emerged or whether changes in extraction methods (enzyme extraction) have resulted in our awareness of their existence. We found that approximately 20% of our enterococcal isolates released material cross-reactive with the group G reagents. These organisms have been present for at least 6 years, and probably for many more. In addition, we found that most strains of Listeria monocytogenes also released antigen cross-reactive with the group G reagent. The clinical significance of these findings is also discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Birch B. R., Keaney M. G., Ganguli L. A. Streptococcus faecalis: group D or group G? Lancet. 1984 Apr 14;1(8381):856–856. doi: 10.1016/s0140-6736(84)92306-7. [DOI] [PubMed] [Google Scholar]
  2. Chang G. T., Ellner P. D. Evaluation of slide agglutination methods for identifying group D streptococci. J Clin Microbiol. 1983 May;17(5):804–806. doi: 10.1128/jcm.17.5.804-806.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Edwards E. A., Phillips I. A., Suiter W. C. Diagnosis of group A streptococcal infections directly from throat secretions. J Clin Microbiol. 1982 Mar;15(3):481–483. doi: 10.1128/jcm.15.3.481-483.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Facklam R. R., Cooksey R. C., Wortham E. C. Evaluation of commercial latex agglutination reagents for grouping streptococci. J Clin Microbiol. 1979 Nov;10(5):641–646. doi: 10.1128/jcm.10.5.641-646.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fujii H., Kamisango K., Nagaoka M., Uchikawa K., Sekikawa I., Yamamoto K., Azuma I. Structural study on teichoic acids of Listeria monocytogenes types 4a and 4d. J Biochem. 1985 Mar;97(3):883–891. doi: 10.1093/oxfordjournals.jbchem.a135130. [DOI] [PubMed] [Google Scholar]
  6. Jones D. E., Kanarek K. S., Angel J. L., Lim D. V. Elimination of multiple reactions of the Phadebact Streptococcus coagglutination test. J Clin Microbiol. 1983 Sep;18(3):526–528. doi: 10.1128/jcm.18.3.526-528.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jones D. E., Kanarek K. S., Lim D. V. Group B streptococcal colonization patterns in mothers and their infants. J Clin Microbiol. 1984 Sep;20(3):438–440. doi: 10.1128/jcm.20.3.438-440.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Leland D. S., Lachapelle R. C., Wlodarski F. M. Method for rapid detection of group B streptococci by coagglutination. J Clin Microbiol. 1978 Apr;7(4):323–326. doi: 10.1128/jcm.7.4.323-326.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McCusker J. J., McCoy E. L., Young C. L., Alamares R., Hirsch L. S. Comparison of Directigen Group A Strep Test with a traditional culture technique for detection of group A beta-hemolytic streptococci. J Clin Microbiol. 1984 Oct;20(4):824–825. doi: 10.1128/jcm.20.4.824-825.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nishimoto G. S., Ellner P. D. Improved recovery and identification of group B streptococci by selective-enrichment and latex agglutination. Med Lab Sci. 1983 Jan;40(1):39–42. [PubMed] [Google Scholar]
  11. Petts D. N. Early detection of streptococci in swabs by latex agglutination before culture. J Clin Microbiol. 1984 Mar;19(3):432–433. doi: 10.1128/jcm.19.3.432-433.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. SEELIGER H. Serologische Kreuzreaktionen zwischen Listeria monocytogenes und Enterokokken. Z Hyg Infektionskr. 1955;141(1):15–24. [PubMed] [Google Scholar]
  13. Shlaes D. M., Toossi Z., Patel A. Comparison of latex agglutination and immunofluorescence for direct Lancefield grouping of streptococci from blood cultures. J Clin Microbiol. 1984 Aug;20(2):195–198. doi: 10.1128/jcm.20.2.195-198.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Szilagyi G., Mayer E., Eidelman A. I. Rapid isolation and identification of group B streptococci from selective broth medium by slide co-agglutination test. J Clin Microbiol. 1978 Oct;8(4):410–412. doi: 10.1128/jcm.8.4.410-412.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Vanzo S. J., Washington J. A., 2nd Evaluation of a rapid latex agglutination test for identification of group D streptococci. J Clin Microbiol. 1984 Sep;20(3):575–576. doi: 10.1128/jcm.20.3.575-576.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wellstood S. Evaluation of Phadebact and Streptex Kits for rapid grouping of streptococci directly from blood cultures. J Clin Microbiol. 1982 Feb;15(2):226–230. doi: 10.1128/jcm.15.2.226-230.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wetkowski M. A., Peterson E. M., de la Maza L. M. Direct testing of blood cultures for detection of streptococcal antigens. J Clin Microbiol. 1982 Jul;16(1):86–91. doi: 10.1128/jcm.16.1.86-91.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

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