Abstract
Levels of amikacin in serum were determined in 106 serum specimens by a latex agglutination inhibition card test and by radioimmunoassay (RIA). Linear regression analysis demonstrated a high degree of correlation between the two assays (latex = 0.95 (RIA) + 0.69; r = 0.97). Assay of three control sera containing 7.5, 15, and 30 micrograms of amikacin per ml on 7 separate days showed good reproducibility with a coefficient of variation of 0 to 11.7% for the latex assay compared with 7.01 to 22.2% for RIA. Recovery of amikacin in spiked sera varied between 93 and 108% for the latex assay compared with 90 and 100% for RIA. Because the procedure involves a titer, the latex agglutination inhibition card test produces results which are categorized rather than results which are continuous. However, it is a rapid and specific method for determining amikacin levels in clinical specimens and is particularly useful when processing small numbers of specimens.
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Selected References
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- Lewis J. E., Nelson J. C., Elder H. A. Amikacin: a rapid and sensitive radioimmunoassay. Antimicrob Agents Chemother. 1975 Jan;7(1):42–45. doi: 10.1128/aac.7.1.42. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maitra S. K., Yoshikawa T. T., Steyn C. M., Guze L. B., Schotz M. C. Amikacin assay in serum by high-performance liquid chromatography. Antimicrob Agents Chemother. 1978 Dec;14(6):880–885. doi: 10.1128/aac.14.6.880. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Marengo P. B., Wilkins J., Overturf G. D. Rapid, specific microbiological assay for amikacin (BB-K8). Antimicrob Agents Chemother. 1974 Oct;6(4):498–500. doi: 10.1128/aac.6.4.498. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith C. R., Baughman K. L., Edwards C. Q., Rogers J. F., Lietman P. S. Controlled comparison of amikacin and gentamicin. N Engl J Med. 1977 Feb 17;296(7):349–353. doi: 10.1056/NEJM197702172960701. [DOI] [PubMed] [Google Scholar]
- Standiford H. C., Bernstein D., Nipper H. C., Caplan E., Tatem B., Hall J. S., Reynolds J. Latex agglutination inhibition card test for gentamicin assay: clinical evaluation and comparison with radioimmunoassay and bioassay. Antimicrob Agents Chemother. 1981 Apr;19(4):620–624. doi: 10.1128/aac.19.4.620. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stevens P., Young L. S., Hewitt W. L. Improved acetylating radioenzymatic assay of amikacin, tobramycin, and sisomicin in serum. Antimicrob Agents Chemother. 1975 Mar;7(3):374–376. doi: 10.1128/aac.7.3.374. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tally F. P., Gorbach S. L. Review of 152 patients with bacteremias treated with amikacin. Am J Med. 1977 Jun;62(6):940–944. doi: 10.1016/0002-9343(77)90665-9. [DOI] [PubMed] [Google Scholar]
- Westgard J. O., Hunt M. R. Use and interpretation of common statistical tests in method-comparison studies. Clin Chem. 1973 Jan;19(1):49–57. [PubMed] [Google Scholar]