Abstract
The Mycotrim-GU (Hana Biologics, Berkeley, Calif.) biphasic culture system and a conventional system were compared for their ability to detect Ureaplasma urealyticum and Mycoplasma species in 100 clinical specimens. Both systems detected 18 Mycoplasma spp. isolates. The average colony detection time was 1.9 days with the Mycotrim-GU and 2.3 days with the conventional system. The Mycotrim-GU agar detected all 33 U. urealyticum isolates recovered in the study, and the conventional agar detected 31. In addition to the U. urealyticum isolates recovered from the agar, there were several specimens that, although they did not grow colonies on the agar, gave an alkaline broth change. Of these specimens, two were found with the conventional system and seven were found with the Mycotrim-GU. The average detection time of U. urealyticum colonies was 2.0 days for the conventional agar and 1.7 days for the Mycotrim-GU. The Mycotrim-GU offers several advantages over the conventional system: it is commercially available, consists of a one-flask system which is ready to use, has a significantly longer shelf life, and is cost competitive. This study showed the Mycotrim-GU to be an effective system for detecting the genital mycoplasmas.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Fiacco V., Miller M. J., Carney E., Martin W. J. Comparison of media for isolation of Ureaplasma urealyticum and genital Mycoplasma species. J Clin Microbiol. 1984 Nov;20(5):862–865. doi: 10.1128/jcm.20.5.862-865.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kundsin R. B., Parreno A., Poulin S. Significance of appropriate techniques and media for isolation and identification of Ureaplasma urealyticum from clinical specimens. J Clin Microbiol. 1978 Oct;8(4):445–453. doi: 10.1128/jcm.8.4.445-453.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Leland D. S., Lapworth M. A., Jones R. B., French M. L. Comparative evaluation of media for isolation of ureaplasma urealyticum and genital mycoplasma species. J Clin Microbiol. 1982 Oct;16(4):709–714. doi: 10.1128/jcm.16.4.709-714.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Razin S., Masover G. K., Palant M., Hayflick L. Morphology of Ureaplasma urealyticum (T-mycoplasma) organisms and colonies. J Bacteriol. 1977 Apr;130(1):464–471. doi: 10.1128/jb.130.1.464-471.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robertson J. A. Bromothymol blue broth: improved medium for detection of Ureaplasma urealyticum (T-strain mycoplasma). J Clin Microbiol. 1978 Feb;7(2):127–132. doi: 10.1128/jcm.7.2.127-132.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robertson J. A., Chen M. H. Effects of manganese on the growth and morphology of Ureaplasma urealyticum. J Clin Microbiol. 1984 Jun;19(6):857–864. doi: 10.1128/jcm.19.6.857-864.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shepard M. C., Lunceford C. D. Differential agar medium (A7) for identification of Ureaplasma urealyticum (human T mycoplasmas) in primary cultures of clinical material. J Clin Microbiol. 1976 Jun;3(6):613–625. doi: 10.1128/jcm.3.6.613-625.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shepard M. C., Lunceford C. D. Urease color test medium U-9 for the detection and identification of "T" mycoplasms in clinical material. Appl Microbiol. 1970 Oct;20(4):539–543. doi: 10.1128/am.20.4.539-543.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Taylor-Robinson D., McCormack W. M. The genital mycoplasmas (first of two parts). N Engl J Med. 1980 May 1;302(18):1003–1010. doi: 10.1056/NEJM198005013021805. [DOI] [PubMed] [Google Scholar]
- Taylor-Robinson D., McCormack W. M. The genital mycoplasmas (second of two parts). N Engl J Med. 1980 May 8;302(19):1063–1067. doi: 10.1056/NEJM198005083021905. [DOI] [PubMed] [Google Scholar]
- Yajko D. M., Balston E., Wood D., Sweet R. L., Hadley W. K. Evaluation of PPLO, A7B, E, and NYC agar media for the isolation of Ureaplasma urealyticum and Mycoplasma species from the genital tract. J Clin Microbiol. 1984 Jan;19(1):73–76. doi: 10.1128/jcm.19.1.73-76.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]