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. 1985 Nov;22(5):808–814. doi: 10.1128/jcm.22.5.808-814.1985

Determination of antibodies to pneumococcal C polysaccharide in patients with community-acquired pneumonia.

H Holmberg, A Krook, A M Sjögren
PMCID: PMC268532  PMID: 4056007

Abstract

The pneumococcal C polysaccharide (PnC) is species specific and believed to be a cell wall component of all capsular types. Antibodies against PnC in human sera have been demonstrated previously, but the question of whether a rise in these antibodies occurs during pneumococcal infections has not been investigated. We used an indirect enzyme-linked immunosorbent assay (ELISA) for the estimation of PnC antibodies in 124 hospital-treated patients with pneumonia. In 3 of 6 patients with pneumococcal bacteremia and in 17 of 44 patients with S. pneumoniae isolated in the blood, sputum, or nasopharynx, a significant rise in antibody levels was recorded, accounting for a sensitivity of 38.6%. Of 35 patients with pneumonia of other known or suspected etiology, 1 gave a positive result, corresponding to a specificity of 97.1%. In addition, 3 of 8 patients with PnC antigen in the sputum as the only etiological finding and 5 of 37 patients with unknown etiology gave positive results. The PnC antibodies did not seem to have any protective capacity against pneumonia caused by pneumococci. The ELISA, in which only one antigen preparation was used, was more simple than other tests in which traditional capsular antigen preparations are used. It might therefore be used as a supplemental method in the diagnosis of pneumococcal pneumonia. The problems involved in expressing serum titers obtained with the ELISA are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ammann A. J., Pelger R. J. Determination of antibody to pneumococcal polysaccharides with chromic chloride-treated human red blood cells and indirect hemagglutination. Appl Microbiol. 1972 Nov;24(5):679–683. doi: 10.1128/am.24.5.679-683.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Austrian R. Pneumococcal infection. Prev Med. 1974 Dec;3(4):443–445. doi: 10.1016/0091-7435(74)90004-8. [DOI] [PubMed] [Google Scholar]
  3. Berntsson E., Broholm K. A., Kaijser B. Serological diagnosis of pneumococcal disease with enzyme-linked immunosorbent assay (ELISA). Scand J Infect Dis. 1978;10(3):177–181. doi: 10.3109/inf.1978.10.issue-3.04. [DOI] [PubMed] [Google Scholar]
  4. Bornstein D. L., Schiffman G., Bernheimer H. P., Austrian R. Capsulation of pneumococcus with soluble C-like (Cs) polysaccharide. I. Biological and genetic properties of Cs pneumococcal strains. J Exp Med. 1968 Dec 1;128(6):1385–1400. doi: 10.1084/jem.128.6.1385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Briles D. E., Nahm M., Schroer K., Davie J., Baker P., Kearney J., Barletta R. Antiphosphocholine antibodies found in normal mouse serum are protective against intravenous infection with type 3 streptococcus pneumoniae. J Exp Med. 1981 Mar 1;153(3):694–705. doi: 10.1084/jem.153.3.694. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Brundish D. E., Baddiley J. Pneumococcal C-substance, a ribitol teichoic acid containing choline phosphate. Biochem J. 1968 Dec;110(3):573–582. doi: 10.1042/bj1100573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Callahan L. T., 3rd, Woodhour A. F., Meeker J. B., Hilleman M. R. Enzyme-linked immunosorbent assay for measurement of antibodies against pneumococcal polysaccharide antigens: comparison with radioimmunoassay. J Clin Microbiol. 1979 Oct;10(4):459–463. doi: 10.1128/jcm.10.4.459-463.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Coonrod J. D., Drennan D. P. Pneumococcal pneumonia: capsular polysaccharide antigenemia and antibody responses. Ann Intern Med. 1976 Mar;84(3):254–260. doi: 10.7326/0003-4819-84-3-254. [DOI] [PubMed] [Google Scholar]
  9. Dee T. H., Schiffman G., Sottile M. I., Rytel M. W. Immunologic studies in pneumococcal disease. J Lab Clin Med. 1977 Jun;89(6):1198–1207. [PubMed] [Google Scholar]
  10. Gearhart P. J., Johnson N. D., Douglas R., Hood L. IgG antibodies to phosphorylcholine exhibit more diversity than their IgM counterparts. Nature. 1981 May 7;291(5810):29–34. doi: 10.1038/291029a0. [DOI] [PubMed] [Google Scholar]
  11. Gray B. M., Dillon H. C., Jr, Briles D. E. Epidemiological studies of Streptococcus pneumoniae in infants: development of antibody to phosphocholine. J Clin Microbiol. 1983 Nov;18(5):1102–1107. doi: 10.1128/jcm.18.5.1102-1107.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Halliday W. J. Immunological paralysis of mice with pneumococcal polysaccharide antigens. Bacteriol Rev. 1971 Sep;35(3):267–289. doi: 10.1128/br.35.3.267-289.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Heidelberger M., Anderson D. G. THE IMMUNE RESPONSE OF HUMAN BEINGS TO BRIEF INFECTIONS WITH PNEUMOCOCCUS. J Clin Invest. 1944 Sep;23(5):607–612. doi: 10.1172/JCI101531. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Holmberg H., Holme T., Krook A., Olsson T., Sjöberg L., Sjögren A. M. Detection of C polysaccharide in Streptococcus pneumoniae in the sputa of pneumonia patients by an enzyme-linked immunosorbent assay. J Clin Microbiol. 1985 Jul;22(1):111–115. doi: 10.1128/jcm.22.1.111-115.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Jennings H. J., Lugowski C., Young N. M. Structure of the complex polysaccharide C-substance from Streptococcus pneumoniae type 1. Biochemistry. 1980 Sep 30;19(20):4712–4719. doi: 10.1021/bi00561a026. [DOI] [PubMed] [Google Scholar]
  16. Kalin M., Lindberg A. A. Antibody response against the type specific capsular polysaccharide in pneumococcal pneumonia measured by enzyme linked immunosorbent assay. Scand J Infect Dis. 1985;17(1):25–32. doi: 10.3109/00365548509070416. [DOI] [PubMed] [Google Scholar]
  17. Kalin M., Lindberg A. A. Diagnosis of pneumococcal pneumonia: a comparison between microscopic examination of expectorate, antigen detection and cultural procedures. Scand J Infect Dis. 1983;15(3):247–255. doi: 10.3109/inf.1983.15.issue-3.04. [DOI] [PubMed] [Google Scholar]
  18. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  19. Macfarlane J. T., Finch R. G., Ward M. J., Macrae A. D. Hospital study of adult community-acquired pneumonia. Lancet. 1982 Jul 31;2(8292):255–258. doi: 10.1016/s0140-6736(82)90334-8. [DOI] [PubMed] [Google Scholar]
  20. Pedersen F. K., Henrichsen J., Sørensen U. S., Nielsen J. L. Anti-C-carbohydrate antibodies after pneumococcal vaccination. Acta Pathol Microbiol Immunol Scand C. 1982 Dec;90(6):353–355. doi: 10.1111/j.1699-0463.1982.tb01462.x. [DOI] [PubMed] [Google Scholar]
  21. Riley I. D., Douglas R. M. An epidemiologic approach to pneumococcal disease. Rev Infect Dis. 1981 Mar-Apr;3(2):233–245. doi: 10.1093/clinids/3.2.233. [DOI] [PubMed] [Google Scholar]
  22. Schiffman G., Bornstein D. L., Austrian R. Capsulation of pneumococcus with soluble cell wall-like polysaccharide. II. Nonidentity of cell wall and soluble cell wall-like polysaccharides derived from the same and from different pneumococcal strains. J Exp Med. 1971 Sep 1;134(3 Pt 1):600–617. doi: 10.1084/jem.134.3.600. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Schiffman G., Douglas R. M., Bonner M. J., Robbins M., Austrian R. A radioimmunoassay for immunologic phenomena in pneumococcal disease and for the antibody response to pneumococcal vaccines. I. Method for the radioimmunoassay of anticapsular antibodies and comparison with other techniques. J Immunol Methods. 1980;33(2):133–144. doi: 10.1016/s0022-1759(80)80004-4. [DOI] [PubMed] [Google Scholar]
  24. Schreiner A., Digranes A., Myking O., Solberg C. O. Wert der transtrachealen Aspiration bei bronchopulmonalen Infektionen im Erwachsenenalter. Infection. 1973;1(3):137–143. doi: 10.1007/BF01641323. [DOI] [PubMed] [Google Scholar]
  25. Szu S. C., Clarke S., Robbins J. B. Protection against pneumococcal infection in mice conferred by phosphocholine-binding antibodies: specificity of the phosphocholine binding and relation to several types. Infect Immun. 1983 Feb;39(2):993–999. doi: 10.1128/iai.39.2.993-999.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Volanakis J. E., Kaplan M. H. Specificity of C-reactive protein for choline phosphate residues of pneumococcal C-polysaccharide. Proc Soc Exp Biol Med. 1971 Feb;136(2):612–614. doi: 10.3181/00379727-136-35323. [DOI] [PubMed] [Google Scholar]
  27. White R. J., Blainey A. D., Harrison K. J., Clarke S. K. Causes of pneumonia presenting to a district general hospital. Thorax. 1981 Aug;36(8):566–570. doi: 10.1136/thx.36.8.566. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Yother J., Forman C., Gray B. M., Briles D. E. Protection of mice from infection with Streptococcus pneumoniae by anti-phosphocholine antibody. Infect Immun. 1982 Apr;36(1):184–188. doi: 10.1128/iai.36.1.184-188.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

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