Abstract
A pool of monoclonal antibodies was compared with polyclonal antiserum for the rapid detection of influenza A virus in 28 clinical specimens by immunofluorescence. Monoclonal antibodies showed higher sensitivity (69 versus 46%) and accuracy (86 versus 75%) and easier slide interpretation than did polyclonal antiserum. The procedure proved useful for rapid detection of a community outbreak of influenza A virus infection.
Full text
PDF


Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Daisy J. A., Lief F. S., Friedman H. M. Rapid diagnosis of influenza A infection by direct immunofluorescence of nasopharyngeal aspirates in adults. J Clin Microbiol. 1979 Jun;9(6):688–692. doi: 10.1128/jcm.9.6.688-692.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LIU C. Rapid diagnosis of human influenza infection from nasal smears by means of fluorescein-labeled antibody. Proc Soc Exp Biol Med. 1956 Aug-Sep;92(4):883–887. doi: 10.3181/00379727-92-22642. [DOI] [PubMed] [Google Scholar]
- Minnich L., Ray C. G. Comparison of direct immunofluorescent staining of clinical specimens for respiratory virus antigens with conventional isolation techniques. J Clin Microbiol. 1980 Sep;12(3):391–394. doi: 10.1128/jcm.12.3.391-394.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schmidt N. J., Ota M., Gallo D., Fox V. L. Monoclonal antibodies for rapid, strain-specific identification of influenza virus isolates. J Clin Microbiol. 1982 Oct;16(4):763–765. doi: 10.1128/jcm.16.4.763-765.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]