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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1986 Jan;23(1):1–5. doi: 10.1128/jcm.23.1.1-5.1986

Comparison of the Quantum II Bacterial Identification System and the AutoMicrobic System for the identification of gram-negative bacilli.

M A Pfaller, M J Bale, K R Schulte, F P Koontz
PMCID: PMC268562  PMID: 3517034

Abstract

The Quantum II Bacterial Identification System (BID; Abbott Laboratories) is a microprocessor-based spectrophotometric system for identification within 4 to 5 h of both enteric and nonenteric gram-negative bacilli. We compared the BID with the AutoMicrobic System (AMS; Vitek Systems, Inc.), using the most recent gram-negative identification card and software (AMS-GNI), for the identification of 501 clinical isolates of gram-negative bacilli, including 382 belonging to the Enterobacteriaceae and 119 nonenteric organisms. The API 20E (Analytab Products) was used as the reference system. The BID correctly identified 375 (98.2%) of the Enterobacteriaceae isolates and 111 (93.2%) of the nonenteric isolates; the AMS-GNI correctly identified 374 (97.9%) and 115 (96.6%) isolates, respectively. The BID identified all isolates within 5 h, whereas the AMS-GNI identified only 35% within this time period. The BID performed comparably to the AMS-GNI for the identification of most gram-negative bacilli. Simplicity, speed, and relatively low reagent cost make the BID a competitive system for many clinical laboratories.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aldridge K. E., Hodges R. L. Correlation studies of entero-set 20, API 20E and conventional media systems for Enterobacteriaceae identification. J Clin Microbiol. 1981 Jan;13(1):120–125. doi: 10.1128/jcm.13.1.120-125.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Appelbaum P. C., Stavitz J., Bentz M. S., von Kuster L. C. Four methods for identification of gram-negative nonfermenting rods: organisms more commonly encountered in clinical specimens. J Clin Microbiol. 1980 Aug;12(2):271–278. doi: 10.1128/jcm.12.2.271-278.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bale M. J., Matsen J. M. Time-motion and cost comparison study of micro-ID, API 20E, and conventional biochemical testing in identification of Enterobacteriaceae. J Clin Microbiol. 1981 Dec;14(6):665–670. doi: 10.1128/jcm.14.6.665-670.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Barry A. L., Gavan T. L., Badal R. E., Telenson M. J. Sensitivity, specificity, and reproducibility of the automicrobic system (with the Enterobacteriaceae-plus biochemical card) for identifying clinical isolates of Gram- negative bacilli. J Clin Microbiol. 1982 Apr;15(4):582–588. doi: 10.1128/jcm.15.4.582-588.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Costigan W. J., Hollick G. E. Use of the Autobac IDX system for rapid identification of Enterobacteriaceae and nonfermentative gram-negative bacilli. J Clin Microbiol. 1984 Feb;19(2):301–302. doi: 10.1128/jcm.19.2.301-302.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. D'Amato R. F., Holmes B., Bottone E. J. The systems approach to diagnostic microbiology. Crit Rev Microbiol. 1981;9(1):1–44. doi: 10.3109/10408418109104485. [DOI] [PubMed] [Google Scholar]
  7. DiPersio J. R., Dyke J. W., Vannest R. D. Evaluation of the updated MS-2 Bacterial Identification system in comparison with the API 20E system. J Clin Microbiol. 1983 Jul;18(1):128–135. doi: 10.1128/jcm.18.1.128-135.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Edberg S. C., Atkinson B., Chambers C., Moore M. H., Palumbo L., Zorzon C. F., Singer J. M. Clinical evaluation of the MICRO-ID, API 20E, and conventional media systems for identification of Enterobacteriacea. J Clin Microbiol. 1979 Aug;10(2):161–167. doi: 10.1128/jcm.10.2.161-167.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jones M. F., Smith T. F., Houglum A. J., Herrmann J. E. Detection of Chlamydia trachomatis in genital specimens by the Chlamydiazyme test. J Clin Microbiol. 1984 Sep;20(3):465–467. doi: 10.1128/jcm.20.3.465-467.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jorgensen J. H., Johnson J. E., Alexander G. A., Paxson R., Alderson G. L. Comparison of automated and rapid manual methods for the same-day identification of Enterobacteriaceae. Am J Clin Pathol. 1983 Jun;79(6):683–687. doi: 10.1093/ajcp/79.6.683. [DOI] [PubMed] [Google Scholar]
  11. Kelly M. T., Latimer J. M. Comparison of the automicrobic system with API, enterotube, micro-ID, micro-media systems, and conventional methods for identification of Enterobacteriaceae. J Clin Microbiol. 1980 Nov;12(5):659–662. doi: 10.1128/jcm.12.5.659-662.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Malloy P. J., Miceika B. G., Ducate M. J. Automated methods in microbiology: II. Identification and susceptibility testing. Am J Med Technol. 1983 May;49(5):313–321. [PubMed] [Google Scholar]
  13. Murray P. R., Gauthier A., Niles A. Evaluation of the Quantum II and Rapid E identification systems. J Clin Microbiol. 1984 Sep;20(3):509–514. doi: 10.1128/jcm.20.3.509-514.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Oberhofer T. R. Comparison of the API 20E and Oxi/Ferm systems in identification of nonfermentative and oxidase-positive fermentative bacteria. J Clin Microbiol. 1979 Feb;9(2):220–226. doi: 10.1128/jcm.9.2.220-226.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Otto L. A., Blachman U. Nonfermentative bacilli: evaluation of three systems for identification. J Clin Microbiol. 1979 Aug;10(2):147–154. doi: 10.1128/jcm.10.2.147-154.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Papasian C. J., Bartholomew W. R., Amsterdam D. Modified enzyme immunoassay for detecting Neisseria gonorrhoeae antigens. J Clin Microbiol. 1984 Oct;20(4):641–643. doi: 10.1128/jcm.20.4.641-643.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Pezzlo M. T. Automated methods for detection of bacteriuria. Am J Med. 1983 Jul 28;75(1B):71–78. doi: 10.1016/0002-9343(83)90075-x. [DOI] [PubMed] [Google Scholar]
  18. Sylvester M. K., Washington J. A., 2nd Evaluation of the Quantum II Microbiology System for bacterial identification. J Clin Microbiol. 1984 Dec;20(6):1196–1197. doi: 10.1128/jcm.20.6.1196-1197.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

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