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. 1986 Jan;23(1):197–198. doi: 10.1128/jcm.23.1.197-198.1986

Method for the typing of Clostridium difficile based on polyacrylamide gel electrophoresis of [35S]methionine-labeled proteins.

S Tabaqchali, S O'Farrell, D Holland, R Silman
PMCID: PMC268603  PMID: 3700603

Abstract

A typing method for Clostridium difficile based on the incorporation of [35S]methionine into cellular proteins, their separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and their visualization by autoradiography is described. On analysis of the radiolabeled-protein profiles, nine distinct groups were observed (A to E and W to Z). The method, which is simple, reproducible, and readily expandable, has been applied in epidemiological studies to demonstrate cross-infection and hospital acquisition of C. difficile.

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Selected References

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