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. 1986 Feb;23(2):212–216. doi: 10.1128/jcm.23.2.212-216.1986

Quantitative estimation by a standardized enzyme-linked immunosorbent assay of human T-cell lymphotropic virus type I antibodies in adult T-cell leukemia and acquired immune deficiency syndrome.

S P Halbert, B Poiesz, A E Friedman-Kien, R Montagna, W A Blattner, M Anken
PMCID: PMC268614  PMID: 3009530

Abstract

Sera from patients with adult T-cell leukemia and asymptomatic carriers of human T-cell lymphotropic virus type I (HTLV-I) from widely separated areas of the world reacted strongly in a standardized quantitative enzyme-linked immunosorbent assay procedure with HTLV-I viral antigen prepared from a strain isolated in the United States. There was a sharp differentiation of the values seen in the patients as compared with a normal population. Of the 35 acquired immune deficiency syndrome patients with Kaposi's sarcoma, only 2 were positive for HTLV-I antibodies in this test, and the distribution of the negative assay values in the other acquired immune deficiency syndrome patient sera was similar to that seen in the normal sera. Sera which contained extremely high levels of antibodies to other unrelated viruses (rubella virus, cytomegalovirus, and herpes simplex virus) all showed negative anti-HTLV-I results, in a pattern similar to the normal sera. Sera from patients with several autoimmune disease (systemic lupus erythematosus, rheumatoid arthritis, thyroiditis) as well as those with infectious mononucleosis or myeloma all also showed the normal distribution of negative results, in spite of the presence of very high levels of the autoantibodies, etc., associated with their illnesses.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arya S. K., Gallo R. C., Hahn B. H., Shaw G. M., Popovic M., Salahuddin S. Z., Wong-Staal F. Homology of genome of AIDS-associated virus with genomes of human T-cell leukemia viruses. Science. 1984 Aug 31;225(4665):927–930. doi: 10.1126/science.6089333. [DOI] [PubMed] [Google Scholar]
  2. Barré-Sinoussi F., Chermann J. C., Rey F., Nugeyre M. T., Chamaret S., Gruest J., Dauguet C., Axler-Blin C., Vézinet-Brun F., Rouzioux C. Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS). Science. 1983 May 20;220(4599):868–871. doi: 10.1126/science.6189183. [DOI] [PubMed] [Google Scholar]
  3. Blattner W. A., Kalyanaraman V. S., Robert-Guroff M., Lister T. A., Galton D. A., Sarin P. S., Crawford M. H., Catovsky D., Greaves M., Gallo R. C. The human type-C retrovirus, HTLV, in Blacks from the Caribbean region, and relationship to adult T-cell leukemia/lymphoma. Int J Cancer. 1982 Sep 15;30(3):257–264. doi: 10.1002/ijc.2910300302. [DOI] [PubMed] [Google Scholar]
  4. Gallo R. C., Salahuddin S. Z., Popovic M., Shearer G. M., Kaplan M., Haynes B. F., Palker T. J., Redfield R., Oleske J., Safai B. Frequent detection and isolation of cytopathic retroviruses (HTLV-III) from patients with AIDS and at risk for AIDS. Science. 1984 May 4;224(4648):500–503. doi: 10.1126/science.6200936. [DOI] [PubMed] [Google Scholar]
  5. Halbert S. P., Anken M., Henle W., Golubjatnikov R. Detection of infectious mononucleosis heterophil antibody by a rapid, standardized enzyme-linked immunosorbent assay procedure. J Clin Microbiol. 1982 Apr;15(4):610–616. doi: 10.1128/jcm.15.4.610-616.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Halbert S. P., Bastomsky C. H., Anken M. A rapid standardized enzyme immunoassay for autoantibodies to thyroglobulin. Clin Chim Acta. 1983 Jan 7;127(1):69–76. doi: 10.1016/0009-8981(83)90076-1. [DOI] [PubMed] [Google Scholar]
  7. Halbert S. P., Karsh J., Anken M. A quantitative enzyme immunoassay for IgM rheumatoid factor using human immunoglobulin G as substrate. Am J Clin Pathol. 1980 Dec;74(6):776–784. doi: 10.1093/ajcp/74.6.776. [DOI] [PubMed] [Google Scholar]
  8. Halbert S. P., Karsh J., Anken M. Studies on autoantibodies to deoxyribonucleic acid and deoxyribonucleoprotein with enzyme-immunoassay (ELISA). J Lab Clin Med. 1981 Jan;97(1):97–111. [PubMed] [Google Scholar]
  9. Hinuma Y., Komoda H., Chosa T., Kondo T., Kohakura M., Takenaka T., Kikuchi M., Ichimaru M., Yunoki K., Sato I. Antibodies to adult T-cell leukemia-virus-associated antigen (ATLA) in sera from patients with ATL and controls in Japan: a nation-wide sero-epidemiologic study. Int J Cancer. 1982 Jun 15;29(6):631–635. doi: 10.1002/ijc.2910290606. [DOI] [PubMed] [Google Scholar]
  10. Ikeda M., Fujino R., Matsui T., Yoshida T., Komoda H., Imai J. A new agglutination test for serum antibodies to adult T-cell leukemia virus. Gan. 1984 Oct;75(10):845–848. [PubMed] [Google Scholar]
  11. Karsh J., Halbert S. P., Anken M., Klima E., Steinberg A. D. Anti-DNA, anti-deoxyribonucleoprotein and rheumatoid factor measured by ELISA in patients with systemic lupus erythematosus, Sjögren's syndrome and rheumatoid arthritis. Int Arch Allergy Appl Immunol. 1982;68(1):60–69. doi: 10.1159/000233068. [DOI] [PubMed] [Google Scholar]
  12. Kiefer D. J., Phelps D. A., Halbert S. P. Normalized enzyme-linked immunosorbent assay for determining immunoglobulin G antibodies to cytomegalovirus. J Clin Microbiol. 1983 Jul;18(1):33–39. doi: 10.1128/jcm.18.1.33-39.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kleeman K. T., Kiefer D. J., Halbert S. P. Rubella antibodies detected by several commercial immunoassays in hemagglutination inhibition-negative sera. J Clin Microbiol. 1983 Nov;18(5):1131–1137. doi: 10.1128/jcm.18.5.1131-1137.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Levy J. A., Hoffman A. D., Kramer S. M., Landis J. A., Shimabukuro J. M., Oshiro L. S. Isolation of lymphocytopathic retroviruses from San Francisco patients with AIDS. Science. 1984 Aug 24;225(4664):840–842. doi: 10.1126/science.6206563. [DOI] [PubMed] [Google Scholar]
  15. Poiesz B. J., Ruscetti F. W., Gazdar A. F., Bunn P. A., Minna J. D., Gallo R. C. Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7415–7419. doi: 10.1073/pnas.77.12.7415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Saxinger C., Gallo R. C. Methods in laboratory investigation. Application of the indirect enzyme-linked immunosorbent assay microtest to the detection and surveillance of human t cell leukemia-lymphoma virus. Lab Invest. 1983 Sep;49(3):371–377. [PubMed] [Google Scholar]
  17. Schüpbach J., Sarngadharan M. G., Gallo R. C. Antigens on HTLV-infected cells recognized by leukemia and AIDS sera are related to HTLV viral glycoprotein. Science. 1984 May 11;224(4649):607–610. doi: 10.1126/science.6324349. [DOI] [PubMed] [Google Scholar]
  18. Taguchi H., Sawada T., Fujishita M., Morimoto T., Niiya K., Miyoshi I. Enzyme-linked immunosorbent assay of antibodies to adult T-cell leukemia-associated antigens. Gan. 1983 Apr;74(2):185–187. [PubMed] [Google Scholar]
  19. Takatsuki K. Adult T-cell leukemia/lymphoma (ATL). Jpn J Med. 1984 Feb;23(1):81–83. doi: 10.2169/internalmedicine1962.23.81. [DOI] [PubMed] [Google Scholar]
  20. Weiss S. H., Goedert J. J., Sarngadharan M. G., Bodner A. J., Gallo R. C., Blattner W. A. Screening test for HTLV-III (AIDS agent) antibodies. Specificity, sensitivity, and applications. JAMA. 1985 Jan 11;253(2):221–225. [PubMed] [Google Scholar]
  21. Yoshida M., Miyoshi I., Hinuma Y. Isolation and characterization of retrovirus from cell lines of human adult T-cell leukemia and its implication in the disease. Proc Natl Acad Sci U S A. 1982 Mar;79(6):2031–2035. doi: 10.1073/pnas.79.6.2031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Yoshida M., Seiki M., Yamaguchi K., Takatsuki K. Monoclonal integration of human T-cell leukemia provirus in all primary tumors of adult T-cell leukemia suggests causative role of human T-cell leukemia virus in the disease. Proc Natl Acad Sci U S A. 1984 Apr;81(8):2534–2537. doi: 10.1073/pnas.81.8.2534. [DOI] [PMC free article] [PubMed] [Google Scholar]

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